General

@ref: 3171

BacDive-ID: 6896

DSM-Number: 7466

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic, Gram-negative

description: Methanothermobacter defluvii ADZ is an anaerobe, thermophilic, Gram-negative archaeon that was isolated from sludge, metacrilic wastes digester.

NCBI tax id

• NCBI tax id: 49339
• Matching level: species

strain history

• @ref: 3171
• history: <- S. V. Kotelnikova and A. Y. Obraztsova; ADZ

doi: 10.13145/bacdive6896.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanobacteria
• order: Methanobacteriales
• family: Methanobacteriaceae
• genus: Methanothermobacter
• species: Methanothermobacter defluvii
• full scientific name: Methanothermobacter defluvii (Kotelnikova et al. 1994) Boone 2002

• synonyms

  • @ref: 20215
  • synonym: Methanobacterium defluvii

@ref: 3171

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter defluvii

full scientific name: Methanothermobacter defluvii (Kotelnikova et al. 1994) Boone 2002

strain designation: ADZ

type strain: yes

Morphology

cell morphology

• @ref: 69480
• gram stain: negative
• confidence: 99.972

Culture and growth conditions

culture medium

• @ref: 3171
• name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141
• composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 3171
• growth: positive
• type: growth
• temperature: 60
• range: thermophilic

Physiology and metabolism

oxygen tolerance

spore formation

Isolation, sampling and environmental information

isolation

• @ref: 3171
• sample type: sludge, metacrilic wastes digester
• country: Russia
• origin.country: RUS

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_188780.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_69115;97_89795;98_122922;99_188780&stattab=map
• Last taxonomy: Methanothermobacter
• 16S sequence: X99046
• Sequence Identity:
• Total samples: 63
• soil counts: 3
• aquatic counts: 47
• animal counts: 8
• plant counts: 5

Safety information

risk assessment

• @ref: 3171
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 3171
• description: M.defluvii 16S rRNA gene
• accession: X99046
• length: 1445
• database: ena
• NCBI tax ID: 49339

Genome sequences

GC content

• @ref: 3171
• GC-content: 62.2

Genome-based predictions

predictions

External links

@ref: 3171

culture collection no.: DSM 7466, ATCC 51443, VKM B-1962

straininfo link

• @ref: 76360
• straininfo: 42986

Reference