Strain identifier

BacDive ID: 6895

Type strain: Yes

Species: Methanothermobacter thermophilus

Strain Designation: M

Strain history: <- VKM <- A.Ya. Obraztsova, M

NCBI tax ID(s): 49341 (species)

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General

@ref: 2716

BacDive-ID: 6895

DSM-Number: 6529

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothermobacter thermophilus M is an anaerobe, thermophilic archaeon that was isolated from thermophilic methane tank.

NCBI tax id

  • NCBI tax id: 49341
  • Matching level: species

strain history

  • @ref: 2716
  • history: <- VKM <- A.Ya. Obraztsova, M

doi: 10.13145/bacdive6895.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanothermobacter
  • species: Methanothermobacter thermophilus
  • full scientific name: Methanothermobacter thermophilus (Laurinavichus et al. 1990) Boone 2002

  • synonyms

    @refsynonym
    20215Methanothermobacter thermophilus
    20215Methanobacterium thermophilum

@ref: 2716

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter thermophilus

full scientific name: Methanothermobacter thermophilus (Laurinavichus et al. 1990) Boone 2002

strain designation: M

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2716
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/119
  • composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

  • @ref: 2716
  • growth: positive
  • type: growth
  • temperature: 60
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2716
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 2716
  • sample type: thermophilic methane tank
  • country: USSR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Condition#Anoxic (anaerobic)
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_188782.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_68809;97_89451;98_122923;99_188782&stattab=map
  • Last taxonomy: Methanothermobacter thermophilus
  • 16S sequence: X99048
  • Sequence Identity:
  • Total samples: 51
  • aquatic counts: 49
  • animal counts: 1
  • plant counts: 1

Safety information

risk assessment

  • @ref: 2716
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.thermophilum 16S rRNA geneX990481432ena49341
2716Methanothermobacter thermophilus partial 16S rRNA gene, strain DSM 6529 type strainLT6262601374ena49341

GC content

  • @ref: 2716
  • GC-content: 44.7

External links

@ref: 2716

culture collection no.: DSM 6529, VKM B-1786

straininfo link

  • @ref: 76359
  • straininfo: 48230

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2716Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6529)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6529
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76359Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48230.1StrainInfo: A central database for resolving microbial strain identifiers