Strain identifier
BacDive ID: 6888
Type strain: 
Species: Methanothermobacter thermautotrophicus
Strain Designation: Z-245
Strain history: <- T.N. Zhilina, Z-245
NCBI tax ID(s): 145262 (species)
version 8.1 (current version)
General
@ref: 1396
BacDive-ID: 6888
DSM-Number: 3720
keywords: genome sequence, Archaea, anaerobe, thermophilic
description: Methanothermobacter thermautotrophicus Z-245 is an anaerobe, thermophilic archaeon that was isolated from manure.
NCBI tax id
- NCBI tax id: 145262
- Matching level: species
strain history
- @ref: 1396
- history: <- T.N. Zhilina, Z-245
doi: 10.13145/bacdive6888.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanothermobacter
- species: Methanothermobacter thermautotrophicus
- full scientific name: Methanothermobacter thermautotrophicus corrig. (Zeikus and Wolfe 1972) Wasserfallen et al. 2000
synonyms
@ref synonym 20215 Methanobacterium thermautotrophicum 20215 Methanobacterium thermalcaliphilum 20215 Methanobacterium thermoautotrophicus 20215 Methanobacterium thermoalcaliphilum 20215 Methanobacterium thermoformicicum 20215 Methanobacterium thermoautotrophicum
@ref: 1396
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanothermobacter
species: Methanothermobacter thermautotrophicus
full scientific name: Methanothermobacter thermautotrophicus (Zeikus and Wolfe 1972) Wasserfallen et al. 2000
strain designation: Z-245
type strain: no
Culture and growth conditions
culture medium
- @ref: 1396
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
- @ref: 1396
- growth: positive
- type: growth
- temperature: 55
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1396
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1396
- sample type: manure
- country: USSR
- continent: Asia
isolation source categories
- Cat1: #Host Body Product
- Cat2: #Gastrointestinal tract
- Cat3: #Feces (Stool)
Safety information
risk assessment
- @ref: 1396
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
- @ref: 66792
- description: Methanothermobacter thermautotrophicus Z-245
- accession: GCA_027555045
- assembly level: complete
- database: ncbi
- NCBI tax ID: 145262
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 85.596 | no |
| flagellated | no | 96.242 | no |
| gram-positive | no | 97.248 | no |
| anaerobic | yes | 98.622 | yes |
| aerobic | no | 96.153 | yes |
| halophile | no | 81.167 | no |
| spore-forming | no | 96.66 | no |
| glucose-ferment | no | 63.265 | no |
| thermophile | yes | 91.099 | yes |
| glucose-util | yes | 72.922 | no |
External links
@ref: 1396
culture collection no.: DSM 3720
straininfo link
- @ref: 76352
- straininfo: 47749
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 1336177 | Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum. | Nolling J, van Eeden FJ, Eggen RI, de Vos WM | Nucleic Acids Res | 10.1093/nar/20.24.6501 | 1992 | Amino Acid Sequence, Archaea/*enzymology/*genetics, Bacterial Proteins/*genetics, Base Sequence, Cloning, Molecular, *DNA Glycosylases, DNA Repair, DNA Restriction Enzymes/*genetics, DNA, Bacterial/chemistry/*genetics, Deoxyribonuclease (Pyrimidine Dimer), Endodeoxyribonucleases/*genetics, Escherichia coli/genetics, Methanobacterium/*enzymology/*genetics, Molecular Sequence Data, N-Glycosyl Hydrolases/*genetics, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Open Reading Frames, *Plasmids, Polymerase Chain Reaction/methods, Sequence Homology, Amino Acid | Genetics |
| Phylogeny | 1380288 | DNA relatedness among some thermophilic members of the genus Methanobacterium: emendation of the species Methanobacterium thermoautotrophicum and rejection of Methanobacterium thermoformicicum as a synonym of Methanobacterium thermoautotrophicum. | Touzel JP, Conway de Macario E, Nolling J, De Vos WM, Zhilina T, Lysenko AM | Int J Syst Bacteriol | 10.1099/00207713-42-3-408 | 1992 | Antigens, Bacterial/immunology, Base Composition, Base Sequence, Cross Reactions, DNA, Bacterial/*chemistry, Methanobacterium/*classification/genetics/immunology, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Bacterial/chemistry, RNA, Ribosomal, 5S/chemistry, Sequence Homology, Nucleic Acid | Genetics |
| Enzymology | 1408820 | Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene. | Nolling J, de Vos WM | Nucleic Acids Res | 10.1093/nar/20.19.5047 | 1992 | Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics/isolation & purification, Deoxyribonucleases, Type II Site-Specific/*genetics, Escherichia coli/genetics, *Genes, Bacterial, Methanobacterium/*enzymology/*genetics, Molecular Sequence Data, Plasmids, Restriction Mapping, Sequence Homology, Amino Acid, Substrate Specificity | Genetics |
| Enzymology | 9006048 | Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245. | Nolling J, Reeve JN | J Bacteriol | 10.1128/jb.179.3.899-908.1997 | 1997 | Bacteriological Techniques, Carbon Dioxide/metabolism, Cloning, Molecular, Formate Dehydrogenases/*genetics, Formates/metabolism, *Gene Expression Regulation, Bacterial, Genes, Bacterial, Hydrogen/metabolism, Methane/metabolism, Methanobacterium/enzymology/*genetics/growth & development, Molecular Sequence Data, *Operon, RNA, Bacterial/analysis, RNA, Messenger/analysis, Sequence Analysis, DNA, Sequence Homology, Species Specificity, *Transcription, Genetic | Metabolism |
| Phylogeny | 10843058 | Caloramator coolhaasii sp. nov., a glutamate-degrading, moderately thermophilic anaerobe. | Plugge CM, Zoetendal EG, Stams AJ | Int J Syst Evol Microbiol | 10.1099/00207713-50-3-1155 | 2000 | Bacteria, Anaerobic/*classification/growth & development/isolation & purification/*metabolism, Base Composition, Biodegradation, Environmental, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Euryarchaeota/growth & development/metabolism, Genes, rRNA, Glutamates/*metabolism, Hydrogen/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Temperature | Metabolism |
| Phylogeny | 11931148 | Gelria glutamica gen. nov., sp. nov., a thermophilic, obligately syntrophic, glutamate-degrading anaerobe. | Plugge CM, Balk M, Zoetendal EG, Stams AJM | Int J Syst Evol Microbiol | 10.1099/00207713-52-2-401 | 2002 | Anaerobiosis, Base Composition, Culture Media, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Euryarchaeota/metabolism, Glutamic Acid/metabolism, Gram-Positive Endospore-Forming Rods/*classification/physiology, Hot Temperature, Molecular Sequence Data, Movement, Oxidation-Reduction, Phylogeny, Proline/metabolism, Propionates/metabolism, Pyruvic Acid, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid | Metabolism |
| Metabolism | 24983531 | Physiological and genetic basis for self-aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2. | Kosaka T, Toh H, Fujiyama A, Sakaki Y, Watanabe K, Meng XY, Hanada S, Toyoda A | Environ Microbiol Rep | 10.1111/1758-2229.12128 | 2013 | Bacterial Adhesion/genetics, Gene Expression Profiling, Genes, Bacterial, Genome, Bacterial, Hydrogen/metabolism, Methane/metabolism, Methanobacteriaceae/isolation & purification/*physiology/ultrastructure, Monosaccharides/metabolism, Oxidation-Reduction, Propionates/*metabolism, Transcription, Genetic | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1396 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3720) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3720 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76352 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47749.1 | StrainInfo: A central database for resolving microbial strain identifiers |