Strain identifier

BacDive ID: 6882

Type strain: Yes

Species: Methanothermobacter thermautotrophicus

Strain Designation: Delta H, DeltaH

Strain history: DSM 1053 <-- J. G. Zeikus ΔH.

NCBI tax ID(s): 145262 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 387

BacDive-ID: 6882

DSM-Number: 1053

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothermobacter thermautotrophicus Delta H is an anaerobe, thermophilic archaeon that was isolated from sewage sludge.

NCBI tax id

  • NCBI tax id: 145262
  • Matching level: species

strain history

@refhistory
387<- J. G. Zeikus, University of Illinois, Urbana, USA; DeltaH <- J. G. Zeikus {1971}
67770DSM 1053 <-- J. G. Zeikus ΔH.

doi: 10.13145/bacdive6882.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanothermobacter
  • species: Methanothermobacter thermautotrophicus
  • full scientific name: Methanothermobacter thermautotrophicus corrig. (Zeikus and Wolfe 1972) Wasserfallen et al. 2000
  • synonyms

    @refsynonym
    20215Methanobacterium thermautotrophicum
    20215Methanobacterium thermalcaliphilum
    20215Methanobacterium thermoautotrophicus
    20215Methanobacterium thermoalcaliphilum
    20215Methanobacterium thermoformicicum
    20215Methanobacterium thermoautotrophicum

@ref: 387

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter thermautotrophicus

full scientific name: Methanothermobacter thermautotrophicus (Zeikus and Wolfe 1972) Wasserfallen et al. 2000

strain designation: Delta H, DeltaH

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
387MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)yeshttps://mediadive.dsmz.de/medium/1523Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water
387METHANOBACTERIUM MEDIUM (DSMZ Medium 119)yeshttps://mediadive.dsmz.de/medium/119Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

@refgrowthtypetemperaturerange
387positivegrowth65thermophilic
67770positivegrowth65thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 387
  • oxygen tolerance: anaerobe

compound production

@refcompound
3877,8 didemethyl-8-hydroxy-5-deazariboflavin
387coenzyme F420

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
387sewage sludgeIllinois, UrbanaUSAUSANorth America
67770Anaerobic sewage-sludge

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Sewage sludge

Safety information

risk assessment

  • @ref: 387
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770M.thermoautotrophicum 5S ribosomal RNAX62857123ena145262
67770M.thermoautotrophicum (DELTA H) gene for 16S rRNAX687201334ena145262

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanothermobacter thermautotrophicus str. Delta HGCA_000008645completencbi187420
66792Methanothermobacter thermautotrophicus Delta H638154510completeimg187420
66792Methanothermobacter thermautotrophicus str. Delta H187420.15completepatric187420
66792Methanothermobacter thermautotrophicus Delta HGCA_027554905completencbi145262

GC content

@refGC-contentmethod
38749.7
38748.6
6777052Buoyant density centrifugation (BD)
6777049.7Buoyant density centrifugation (BD)
6777050.7thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno84.854no
flagellatedno96.206no
gram-positiveno97.348no
anaerobicyes98.682yes
halophileno84.913no
spore-formingno96.645no
glucose-utilyes73.478no
aerobicno96.508no
thermophileyes90.251yes
glucose-fermentno63.601no

External links

@ref: 387

culture collection no.: DSM 1053, ATCC 29096, JCM 10044, NBRC 100330, OCM 143, VKM B-1908

straininfo link

  • @ref: 76346
  • straininfo: 47306

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1380288DNA relatedness among some thermophilic members of the genus Methanobacterium: emendation of the species Methanobacterium thermoautotrophicum and rejection of Methanobacterium thermoformicicum as a synonym of Methanobacterium thermoautotrophicum.Touzel JP, Conway de Macario E, Nolling J, De Vos WM, Zhilina T, Lysenko AMInt J Syst Bacteriol10.1099/00207713-42-3-4081992Antigens, Bacterial/immunology, Base Composition, Base Sequence, Cross Reactions, DNA, Bacterial/*chemistry, Methanobacterium/*classification/genetics/immunology, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Bacterial/chemistry, RNA, Ribosomal, 5S/chemistry, Sequence Homology, Nucleic AcidGenetics
Enzymology6300837Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.Kojima N, Fox JA, Hausinger RP, Daniels L, Orme-Johnson WH, Walsh CProc Natl Acad Sci U S A10.1073/pnas.80.2.3781983Coenzymes, Electron Spin Resonance Spectroscopy, Euryarchaeota/*enzymology, Flavins, Hydrogenase, Kinetics, Nickel/*analysis, Oxidation-Reduction, Oxidoreductases/*metabolism, *Riboflavin/*analogs & derivativesMetabolism
Enzymology6314095A plasmid in the archaebacterium Methanobacterium thermoautotrophicum.Meile L, Kiener A, Leisinger TMol Gen Genet10.1007/BF004257661983DNA Restriction Enzymes, DNA, Bacterial/genetics, Euryarchaeota/*genetics, *Plasmids
Enzymology7400098Microbiology of methanogenesis in thermal, volcanic environments.Zeikus JG, Ben-Bassat A, Hegge PWJ Bacteriol10.1128/jb.143.1.432-440.19801980Anaerobiosis, Bacteria/isolation & purification, Clostridium/isolation & purification, Euryarchaeota/cytology/isolation & purification/*metabolism, Hot Temperature, Hydrogen/metabolism, Methane/*biosynthesis, *Water Microbiology, WyomingPhylogeny
Enzymology9478969Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaH.Mukhopadhyay B, Stoddard SF, Wolfe RSJ Biol Chem10.1074/jbc.273.9.51551998Amino Acid Sequence, Bacterial Proteins/genetics, Base Sequence, Binding Sites, Biotinylation, Carbon-Nitrogen Ligases/genetics, Cloning, Molecular, *Escherichia coli Proteins, *Gene Expression Regulation, Archaeal, *Gene Expression Regulation, Enzymologic, Genes, Archaeal, Methanobacterium/*enzymology/genetics, Molecular Sequence Data, Pyruvate Carboxylase/*genetics/isolation & purification/*metabolism, *Repressor Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Species Specificity, Substrate Specificity, *Transcription FactorsPhylogeny
Metabolism9565696Methanopterin biosynthesis: methylation of the biosynthetic intermediates.White RHBiochim Biophys Acta10.1016/s0304-4165(97)00148-71998Azo Compounds/chemistry/metabolism, Binding Sites, Glutarates/metabolism, Methanobacterium/chemistry/metabolism, Methylation, Phosphorylation, Pterins/analysis/chemistry/*metabolism, Species Specificity, Subcellular Fractions/chemistry/metabolism, TritiumPhylogeny
Phylogeny9573152Isolation and characterization of Methanobacterium thermoautotrophicum DeltaH mutants unable to grow under hydrogen-deprived conditions.Pennings JL, Keltjens JT, Vogels GDJ Bacteriol10.1128/JB.180.10.2676-2681.19981998Adenosine Monophosphate/analogs & derivatives/analysis, Bacterial Proteins/*metabolism, Colony Count, Microbial, Flavins, Hydrogen/physiology, Methanobacterium/enzymology/growth & development/*isolation & purification, Mutagenesis, RNA, Messenger/*metabolism, Riboflavin/analogs & derivatives/analysisMetabolism
Enzymology9639601Two malate dehydrogenases in Methanobacterium thermoautotrophicum.Thompson H, Tersteegen A, Thauer RK, Hedderich RArch Microbiol10.1007/s0020300506121998Acetates/metabolism, Amino Acid Sequence, Archaeal Proteins/classification/*genetics/isolation & purification/metabolism, Bacillus subtilis/genetics, Electrophoresis, Polyacrylamide Gel, Euryarchaeota/*enzymology/genetics, Malate Dehydrogenase/classification/*genetics/isolation & purification/metabolism, Malates/metabolism, Molecular Sequence Data, NAD/metabolism, NADP/metabolism, Phylogeny, Sequence AlignmentMetabolism
Metabolism10400604Methanobacterium thermoautotrophicum RNA polymerase and transcription in vitro.Darcy TJ, Hausner W, Awery DE, Edwards AM, Thomm M, Reeve JNJ Bacteriol10.1128/JB.181.14.4424-4429.19991999Amino Acid Sequence, *Archaeal Proteins, Centrifugation, Density Gradient, DNA-Binding Proteins/genetics/metabolism, DNA-Directed RNA Polymerases/*genetics/isolation & purification/*metabolism, Electrophoresis, Polyacrylamide Gel, *Gene Expression Regulation, Archaeal, Hydrogen/metabolism, Methane/metabolism, Methanobacterium/enzymology/*genetics/metabolism, Molecular Sequence Data, Nuclear Proteins/genetics/metabolism, Precipitin Tests, TATA-Box Binding Protein, *Transcription Factor TFIIB, Transcription Factors/genetics/metabolism, *Transcription, GeneticEnzymology
Enzymology10497247Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH.Kelman Z, Pietrokovski S, Hurwitz JJ Biol Chem10.1074/jbc.274.40.287511999Amino Acid Sequence, Base Sequence, Chromatography, Affinity, Cloning, Molecular, DNA Polymerase beta/genetics/*isolation & purification/metabolism, DNA Replication, DNA-Binding Proteins/metabolism, Electrophoresis, Polyacrylamide Gel, Methanobacterium/*enzymology/genetics, Molecular Sequence Data, Oligonucleotides, Recombinant Proteins/genetics/isolation & purification/metabolismGenetics
Enzymology10515911A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum.Smith KS, Ferry JGJ Bacteriol10.1128/JB.181.20.6247-6253.19991999Amino Acid Sequence, Archaeal Proteins/*classification/genetics/isolation & purification, Carbon Dioxide/metabolism, Carbonic Anhydrase Inhibitors, Carbonic Anhydrases/*classification/genetics/isolation & purification, Enzyme Stability, Escherichia coli/genetics, Hot Temperature, Methanobacterium/*enzymology/genetics, Molecular Sequence Data, Plant Proteins/genetics, Recombinant Proteins/classification/isolation & purification, Sequence Homology, Amino Acid, Water/metabolismPhylogeny
Metabolism10611290The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum DeltaH contains DNA helicase activity.Kelman Z, Lee JK, Hurwitz JProc Natl Acad Sci U S A10.1073/pnas.96.26.147831999Adenosine Triphosphatases/metabolism, Adenosine Triphosphate/metabolism, Archaeal Proteins/*metabolism, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosomes, Archaeal/metabolism, DNA Helicases/*metabolism, DNA Replication, DNA, Archaeal/metabolism, DNA, Single-Stranded, DNA-Binding Proteins, Eukaryotic Cells/enzymology, Methanobacterium/*enzymology, Minichromosome Maintenance Complex Component 4, Minichromosome Maintenance Complex Component 6, Minichromosome Maintenance Complex Component 7, Nuclear Proteins, Protein Conformation, *Saccharomyces cerevisiae ProteinsGenetics
Genetics10702304A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH.Kelman Z, Hurwitz JJ Biol Chem10.1074/jbc.275.10.73272000Adenosine Triphosphatases/metabolism, Amino Acid Sequence, DNA/biosynthesis, *DNA Replication, DNA-Binding Proteins/*genetics/isolation & purification/physiology, DNA-Directed DNA Polymerase/*chemistry/genetics, *Homeodomain Proteins, Methanobacterium/*genetics, Minor Histocompatibility Antigens, Molecular Sequence Data, Proliferating Cell Nuclear Antigen/*genetics/isolation & purification, *Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins/isolation & purification, Replication Protein C, *Repressor Proteins, *Saccharomyces cerevisiae ProteinsEnzymology
Enzymology10894731Identification of an alternative nucleoside triphosphate: 5'-deoxyadenosylcobinamide phosphate nucleotidyltransferase in Methanobacterium thermoautotrophicum delta H.Thomas MG, Escalante-Semerena JCJ Bacteriol10.1128/JB.182.15.4227-4233.20002000Amino Acid Sequence, *Archaeal Proteins, Catalysis, Cobamides/biosynthesis/metabolism, Electrophoresis, Polyacrylamide Gel, Gene Library, Guanosine Diphosphate/analogs & derivatives/metabolism, Methanobacterium/*enzymology, Molecular Sequence Data, *Multienzyme Complexes, Nucleotidyltransferases/chemistry/*isolation & purification/metabolism, Open Reading Frames, Pentosyltransferases/genetics/*metabolism, Salmonella enterica/enzymologyMetabolism
Metabolism11053387A DNA ligase from a hyperthermophilic archaeon with unique cofactor specificity.Nakatani M, Ezaki S, Atomi H, Imanaka TJ Bacteriol10.1128/JB.182.22.6424-6433.20002000Adenosine Triphosphate/metabolism, Amino Acid Sequence, Archaeal Proteins/chemistry/*genetics/metabolism, Cations, Divalent, Cloning, Molecular, DNA Ligases/chemistry/*genetics/metabolism, Hydrogen-Ion Concentration, Magnesium/metabolism, Molecular Sequence Data, NAD+ Nucleosidase/metabolism, Phylogeny, Sequence Alignment, Temperature, Thermococcus/enzymology/*geneticsEnzymology
Metabolism11292347Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.Lee BI, Chang C, Cho SJ, Eom SH, Kim KK, Yu YG, Suh SWJ Mol Biol10.1006/jmbi.2001.45322001Allosteric Regulation, Amino Acid Sequence, Arginine/metabolism, Binding Sites, Crystallography, X-Ray, Dimerization, Genes, Archaeal/genetics, Glycine/metabolism, Hydrogen Bonding, L-Lactate Dehydrogenase/*chemistry/classification/genetics/metabolism, Malate Dehydrogenase/*chemistry/classification/genetics/metabolism, Methanococcus/*enzymology/genetics, Models, Molecular, Molecular Sequence Data, NAD/metabolism, NADP/metabolism, Protein Folding, Protein Structure, Quaternary, Protein Subunits, Sequence Alignment, Substrate SpecificityEnzymology
Metabolism11576687Convenient fluorescence-based methods to measure membrane potential and intracellular pH in the Archaeon Methanobacterium thermoautotrophicum.de Poorter LM, Keltjens JTJ Microbiol Methods10.1016/s0167-7012(01)00312-82001Barbiturates/metabolism, Calibration, Culture Media, Cytoplasm/metabolism, Fluorescence, Hydrogen-Ion Concentration, Isoxazoles/metabolism, *Membrane Potentials, Methanobacterium/cytology/*physiology, Proton-Motive Force/physiology, Riboflavin/*analogs & derivatives/*metabolism, Spectrometry, Fluorescence/*methodsCultivation
Metabolism11606589The zinc finger domain of the archaeal minichromosome maintenance protein is required for helicase activity.Poplawski A, Grabowski B, Long SE, Kelman ZJ Biol Chem10.1074/jbc.M1085192002001Amino Acid Sequence, Animals, Archaeal Proteins/*chemistry/genetics/*metabolism, DNA/metabolism, DNA Helicases/*chemistry/genetics/*metabolism, DNA-Binding Proteins/genetics/metabolism, Methanobacterium/*enzymology/genetics, Molecular Sequence Data, Mutation, Recombinant Proteins/chemistry/metabolism, Sequence Alignment, *Zinc FingersGenetics
Metabolism11872465Differential expression of methanogenesis genes of Methanothermobacter thermoautotrophicus (formerly Methanobacterium thermoautotrophicum) in pure culture and in cocultures with fatty acid-oxidizing syntrophs.Luo HW, Zhang H, Suzuki T, Hattori S, Kamagata YAppl Environ Microbiol10.1128/AEM.68.3.1173-1179.20022002Acetates/metabolism, Archaeal Proteins/*genetics/*metabolism, Carbon Dioxide/metabolism, Culture Media, Euryarchaeota/growth & development/metabolism, Gene Expression Regulation, Archaeal, Hydrogen/metabolism, Methane/*metabolism, Methanobacteriaceae/enzymology/genetics/*growth & development, Oxidation-Reduction, Transcription, GeneticCultivation
Metabolism12486068The cobY gene of the archaeon Halobacterium sp. strain NRC-1 is required for de novo cobamide synthesis.Woodson JD, Peck RF, Krebs MP, Escalante-Semerena JCJ Bacteriol10.1128/JB.185.1.311-316.20032003Archaeal Proteins/genetics/metabolism, Cobamides/*biosynthesis, Colony Count, Microbial, Culture Media, Genetic Complementation Test, Halobacterium/*enzymology/genetics/growth & development, Nucleotidyltransferases/*genetics/*metabolismCultivation
Phylogeny14529955Improved strategy for presumptive identification of methanogens using 16S riboprinting.Wright AD, Pimm CJ Microbiol Methods10.1016/s0167-7012(03)00169-62003Base Sequence, DNA, Archaeal/chemistry/genetics, Methanobacteriales/classification/*genetics, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/chemistry/*genetics, Ribotyping/*methods, Sequence Analysis, DNAGenetics
Metabolism16332758Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus.Ishii S, Kosaka T, Hori K, Hotta Y, Watanabe KAppl Environ Microbiol10.1128/AEM.71.12.7838-7845.20052005Clostridium/cytology/growth & development/*metabolism, Fumarates/metabolism, Hydrogen/*metabolism, Kinetics, Methanobacteriaceae/cytology/growth & development/*metabolism, Microscopy, Electron, Scanning, Species SpecificityEnzymology
16346644Comparison of assimilatory organic nitrogen, sulfur, and carbon sources for growth of methanobacterium species.Bhatnagar L, Jain MK, Aubert JP, Zeikus JGAppl Environ Microbiol10.1128/aem.48.4.785-790.19841984
Biotechnology16347389Production of ethane, ethylene, and acetylene from halogenated hydrocarbons by methanogenic bacteria.Belay N, Daniels LAppl Environ Microbiol10.1128/aem.53.7.1604-1610.19871987
16347656Hydrogen partial pressures in a thermophilic acetate-oxidizing methanogenic coculture.Lee MJ, Zinder SHAppl Environ Microbiol10.1128/aem.54.6.1457-1461.19881988
Metabolism16348508Diversity and population dynamics of methanogenic bacteria in a granular consortium.Visser FA, van Lier JB, Macario AJ, Conway de Macario EAppl Environ Microbiol10.1128/aem.57.6.1728-1734.19911991Antibodies, Archaeal/metabolism, Antigens, Archaeal/analysis, *Biodiversity, Bioreactors/*microbiology, Colony Count, Microbial, Euryarchaeota/*physiology, Sewage/*microbiology
16348632Enrichment of Thermophilic Propionate-Oxidizing Bacteria in Syntrophy with Methanobacterium thermoautotrophicum or Methanobacterium thermoformicicum.Stams AJ, Grolle KC, Frijters CT, Van Lier JBAppl Environ Microbiol10.1128/aem.58.1.346-352.19921992
16349232Identification, Biosynthesis, and Function of 1,3,4,6-Hexanetetracarboxylic Acid in Methanobacterium thermoautotrophicum DeltaH.Gorkovenko A, Roberts MF, White RHAppl Environ Microbiol10.1128/aem.60.4.1249-1253.19941994
Cultivation16640584Methanogenesis is Ca2+ dependent in Methanothermobacter thermautotrophicus strain DeltaH.Vancek M, Vidova M, Majernik AI, Smigan PFEMS Microbiol Lett10.1111/j.1574-6968.2006.00232.x2006Adenosine Triphosphate/biosynthesis, Biological Transport/physiology, Calcium/antagonists & inhibitors/*physiology, Calcium Channel Blockers/pharmacology, Culture Media, Egtazic Acid/pharmacology, Methane/*biosynthesis, Methanobacteriaceae/drug effects/growth & development/*metabolism, Ruthenium Red/pharmacologyPathogenicity
16887685Biochemical analysis of neomycin-resistance in the methanoarchaeon Methanothermobacter thermautotrophicus and some implications for energetic processes in this strain.Majernik A, Cubonova L, Polak P, Smigan P, Greksak MAnaerobe10.1016/S1075-9964(03)00042-82003
Metabolism18036179Comparative transcriptome analysis of responses of Methanothermobacter thermautotrophicus to different environmental stimuli.Kato S, Kosaka T, Watanabe KEnviron Microbiol10.1111/j.1462-2920.2007.01508.x2007Ammonia, Anaerobiosis, Environmental Microbiology, Gene Expression Profiling, *Gene Expression Regulation, Bacterial, Hydrogen/metabolism, Hydrogen Peroxide, Hydrogen-Ion Concentration, Methane/metabolism, Methanobacteriaceae/*genetics/metabolism, Temperature, Up-RegulationTranscriptome
Enzymology18757575Specific DNA binding of a potential transcriptional regulator, inosine 5'-monophosphate dehydrogenase-related protein VII, to the promoter region of a methyl coenzyme m reductase I-encoding operon retrieved from Methanothermobacter thermautotrophicus strain DeltaH.Shinzato N, Enoki M, Sato H, Nakamura K, Matsui T, Kamagata YAppl Environ Microbiol10.1128/AEM.02155-072008Archaeal Proteins/genetics/isolation & purification/*metabolism, DNA, Archaeal/*metabolism, DNA-Binding Proteins/genetics/isolation & purification/*metabolism, Electrophoretic Mobility Shift Assay, *Gene Expression Regulation, Archaeal, IMP Dehydrogenase/genetics/isolation & purification/metabolism, Methanobacteriaceae/*physiology, Operon, Oxidoreductases/*biosynthesis, *Promoter Regions, Genetic, Protein Binding, Protein Structure, Tertiary, Transcription Factors/genetics/isolation & purification/metabolismMetabolism
Phylogeny18984690Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio.Sekiguchi Y, Muramatsu M, Imachi H, Narihiro T, Ohashi A, Harada H, Hanada S, Kamagata YInt J Syst Evol Microbiol10.1099/ijs.0.2008/000893-02008Anaerobiosis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, *Hot Temperature, In Situ Hybridization, Fluorescence, Methane/*metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification/physiology, Waste Disposal, Fluid/methodsStress
Metabolism19162025Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus DeltaH.Popp SL, Reinstein JFEBS Lett10.1016/j.febslet.2008.12.0622009Adenosine Triphosphatases/genetics/metabolism, Archaeal Proteins/genetics/*metabolism, Enzyme Stability/drug effects, Genes, Reporter/genetics, Guanidine/pharmacology, Methanobacteriaceae/*enzymology/genetics, Molecular Chaperones/genetics/*metabolism, Protein FoldingEnzymology
Metabolism19240141DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision.Schomacher L, Chong JP, McDermott P, Kramer W, Fritz HJNucleic Acids Res10.1093/nar/gkp1022009Archaeal Proteins/*metabolism, DNA Ligases/metabolism, *DNA Repair, DNA Repair Enzymes/*metabolism, DNA-Directed DNA Polymerase/metabolism, Endodeoxyribonucleases/*metabolism, Flap Endonucleases/metabolism, Methanobacteriaceae/*enzymology, Uracil/*metabolismEnzymology
Metabolism19618917An examination of the relationship between active site loop size and thermodynamic activation parameters for orotidine 5'-monophosphate decarboxylase from mesophilic and thermophilic organisms.Toth K, Amyes TL, Wood BM, Chan KK, Gerlt JA, Richard JPBiochemistry10.1021/bi901064k2009Catalysis, Catalytic Domain, Crystallography, X-Ray, Entropy, Enzyme Activation/physiology, Escherichia coli Proteins/chemistry/metabolism, Methanobacteriaceae/enzymology, Orotidine-5'-Phosphate Decarboxylase/*chemistry/*metabolism, Protein Structure, Secondary, Saccharomyces cerevisiae Proteins/chemistry/metabolism, Structural Homology, Protein, Substrate Specificity, *Thermodynamics, Transition TemperatureEnzymology
Metabolism20129830Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components.Schomacher L, Schurer KA, Ciirdaeva E, McDermott P, Chong JP, Kramer W, Fritz HJDNA Repair (Amst)10.1016/j.dnarep.2010.01.0042010Archaeal Proteins/genetics/*metabolism, DNA Ligase ATP, DNA Ligases/metabolism, *DNA Repair, DNA, Archaeal/*metabolism, Methanobacteriaceae/*metabolism, Models, Biological, Uracil/*metabolism, Uracil-DNA Glycosidase/metabolism
Metabolism20434457Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA.Lakomek K, Dickmanns A, Ciirdaeva E, Schomacher L, Ficner RJ Mol Biol10.1016/j.jmb.2010.04.0442010Archaeal Proteins/chemistry/genetics/metabolism, Base Sequence, Catalytic Domain, Crystallography, X-Ray, DNA Repair, DNA, Archaeal/*chemistry/genetics/*metabolism, DNA-(Apurinic or Apyrimidinic Site) Lyase/metabolism, Deoxyribonuclease I/*chemistry/genetics/*metabolism, Exodeoxyribonucleases/chemistry/metabolism, Humans, Macromolecular Substances/chemistry, Methanobacteriaceae/*enzymology/genetics, Models, Molecular, Mutant Proteins/chemistry/genetics/metabolism, Nucleic Acid Conformation, Protein Conformation, Static Electricity, Substrate SpecificityPathogenicity
Phylogeny21571939Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically.Zhang F, Liu X, Dong XInt J Syst Evol Microbiol10.1099/ijs.0.033456-02011Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, Coculture Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Gram-Positive Bacteria/genetics/metabolism, Hot Springs/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism
Metabolism21625642Mth10b, a unique member of the Sac10b family, does not bind nucleic acid.Liu YF, Zhang N, Yao HW, Pan XM, Ge MPLoS One10.1371/journal.pone.00199772011Amino Acid Sequence, Archaeal Proteins/chemistry/*metabolism, Chromatography, Liquid, Dimerization, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Nucleic Acids/*metabolism, Sequence Homology, Amino AcidEnzymology
Phylogeny21705537Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field.Cheng L, Dai L, Li X, Zhang H, Lu YAppl Environ Microbiol10.1128/AEM.00210-112011Acetates/metabolism, Anaerobiosis, Bacterial Typing Techniques/*methods, Base Composition, Base Sequence, Carbon/metabolism, China, DNA, Bacterial/analysis/chemistry/genetics, DNA, Ribosomal/analysis/genetics, Methane/biosynthesis, Methanobacteriaceae/classification/genetics/growth & development/*isolation & purification, Molecular Sequence Data, Oil and Gas Fields/*microbiology, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TemperatureMetabolism
Metabolism21904627Comparative proteomic analysis of Methanothermobacter themautotrophicus DeltaH in pure culture and in co-culture with a butyrate-oxidizing bacterium.Enoki M, Shinzato N, Sato H, Nakamura K, Kamagata YPLoS One10.1371/journal.pone.00243092011Bacteria/*metabolism, Bacterial Proteins/metabolism, Butyrates/*metabolism, Coculture Techniques, Methanobacteriaceae/*metabolism, Proteomics/*methods
Metabolism22511977Molecular mechanism underlying the interaction of typical Sac10b family proteins with DNA.Liu YF, Zhang N, Liu X, Wang X, Wang ZX, Chen Y, Yao HW, Ge M, Pan XMPLoS One10.1371/journal.pone.00349862012Amino Acid Sequence, Archaeal Proteins/*chemistry, Binding Sites, DNA, Archaeal/chemistry/metabolism, DNA-Binding Proteins/*chemistry/metabolism, Methanobacterium/genetics/*metabolism, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Sequence AlignmentGenetics
Phylogeny22561588Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic, thermophilic methanogen isolated from gas-associated formation water of a natural gas field.Nakamura K, Takahashi A, Mori C, Tamaki H, Mochimaru H, Nakamura K, Takamizawa K, Kamagata YInt J Syst Evol Microbiol10.1099/ijs.0.041681-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Japan, Lipids/analysis, Methane/metabolism, Methanobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Natural Gas/microbiology, Oil and Gas Fields/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyGenetics
Metabolism22652087Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen.Sasaki D, Morita M, Sasaki K, Watanabe A, Ohmura NJ Biosci Bioeng10.1016/j.jbiosc.2012.05.0022012Bacteria/metabolism, Biodegradation, Environmental, Bioreactors, Cellulose/*metabolism, Clostridium/*metabolism, *Garbage, Metabolic Networks and Pathways, Methane/*metabolism, Methanobacteriaceae/*metabolism, Molecular Sequence DataGenetics
Stress23300880Without salt, the 'thermophilic' protein Mth10b is just mesophilic.Zhang N, Pan XM, Ge MPLoS One10.1371/journal.pone.00531252012Bacterial Proteins/*metabolism, Escherichia coli, Hot Temperature, Methanobacterium, Protein Conformation, *Protein Folding, *Sodium ChlorideProteome
Metabolism24983531Physiological and genetic basis for self-aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2.Kosaka T, Toh H, Fujiyama A, Sakaki Y, Watanabe K, Meng XY, Hanada S, Toyoda AEnviron Microbiol Rep10.1111/1758-2229.121282013Bacterial Adhesion/genetics, Gene Expression Profiling, Genes, Bacterial, Genome, Bacterial, Hydrogen/metabolism, Methane/metabolism, Methanobacteriaceae/isolation & purification/*physiology/ultrastructure, Monosaccharides/metabolism, Oxidation-Reduction, Propionates/*metabolism, Transcription, GeneticGenetics
25657645Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability.Yoshinaga MY, Gagen EJ, Wormer L, Broda NK, Meador TB, Wendt J, Thomm M, Hinrichs KUFront Microbiol10.3389/fmicb.2015.000052015
Genetics27864137Intergenomic evolution and metabolic cross-talk between rumen and thermophilic autotrophic methanogenic archaea.Bharathi M, Chellapandi PMol Phylogenet Evol10.1016/j.ympev.2016.11.0082016Animals, Archaea/*genetics, *Autotrophic Processes, DNA Replication/genetics, *Evolution, Molecular, Genetic Markers, Genetic Variation, *Genome, Archaeal, Metabolic Networks and Pathways/genetics, Methane/*metabolism, Methanobacteriaceae/genetics, Phylogeny, Rumen/*microbiology, SyntenyPhylogeny
Metabolism30445075Functional annotation of operome from Methanothermobacter thermautotrophicus DeltaH: An insight to metabolic gap filling.Prathiviraj R, Chellapandi PInt J Biol Macromol10.1016/j.ijbiomac.2018.11.1002018*Bacterial Proteins/genetics/metabolism, Methanobacteriaceae/genetics/metabolism, *Molecular Sequence Annotation, *Multigene Family
Phylogeny32375973Zhaonella formicivorans gen. nov., sp. nov., an anaerobic formate-utilizing bacterium isolated from Shengli oilfield, and proposal of four novel families and Moorellales ord. nov. in the phylum Firmicutes.Lv XM, Yang M, Dai LR, Tu B, Chang C, Huang Y, Deng Y, Lawson PA, Zhang H, Cheng L, Tang YQInt J Syst Evol Microbiol10.1099/ijsem.0.0041782020Anaerobiosis, Bacteria, Anaerobic/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Firmicutes/*classification/isolation & purification, Formates/*metabolism, Oil and Gas Fields/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Phylogeny32504807Comparative genomic analysis reveals starvation survival systems in Methanothermobacter thermautotrophicus DeltaH.Prathiviraj R, Chellapandi PAnaerobe10.1016/j.anaerobe.2020.1022162020Comparative Genomic Hybridization, DNA, Archaeal/genetics, Genome, Archaeal, Metabolic Networks and Pathways/*genetics, Methanobacteriaceae/*genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Stress, Physiological/*geneticsGenetics
Metabolism34809461A Shuttle-Vector System Allows Heterologous Gene Expression in the Thermophilic Methanogen Methanothermobacter thermautotrophicus DeltaH.Fink C, Beblawy S, Enkerlin AM, Muhling L, Angenent LT, Molitor BmBio10.1128/mBio.02766-212021Bacterial Proteins/genetics/metabolism, Conjugation, Genetic, Escherichia coli/genetics/metabolism, Galactosidases/genetics/metabolism, *Gene Expression, Genetic Vectors/*genetics/metabolism, Geobacillus/*enzymology/genetics, Methane/metabolism, Methanobacteriaceae/*genetics/growth & development/metabolismEnzymology
Pathogenicity36125746An Interdomain Conjugation Protocol for Plasmid-DNA Transfer into Methanothermobacter thermautotrophicus DeltaH.Fink C, Angenent LT, Molitor BMethods Mol Biol10.1007/978-1-0716-2445-6_72022Anaerobiosis, Chemical Phenomena, Humans, *Methane, *Methanobacteriaceae/genetics, Plasmids/genetics
Metabolism36361694Functional Characterization and Screening of Promiscuous Kinases and Isopentenyl Phosphate Kinases for the Synthesis of DMAPP via a One-Pot Enzymatic Cascade.Qiu C, Liu Y, Wu Y, Zhao L, Pei JInt J Mol Sci10.3390/ijms2321129042022*Phosphates/metabolism, *Hemiterpenes, Escherichia coli/metabolism, Organophosphates/metabolism, Terpenes/metabolism, Flavonoids/metabolism

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20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
76346Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47306.1StrainInfo: A central database for resolving microbial strain identifiers