Strain identifier

BacDive ID: 6881

Type strain: Yes

Species: Listeria rocourtiae

Strain Designation: 700284/02

Strain history: CIP <- 2008, A. Leclercq, Inst. Pasteur, France: strain CLIP 2007/00563 <- 2007, F. Allerberger, AGES, Vienna, Austria: strain 700284/02

NCBI tax ID(s): 1265846 (strain), 647910 (species)

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General

@ref: 16137

BacDive-ID: 6881

DSM-Number: 22097

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Listeria rocourtiae 700284/02 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from precut lettuce.

NCBI tax id

NCBI tax idMatching level
647910species
1265846strain

strain history

@refhistory
16137<- CIP <- F. Allerberger, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria; CLIP 2007/00563
122245CIP <- 2008, A. Leclercq, Inst. Pasteur, France: strain CLIP 2007/00563 <- 2007, F. Allerberger, AGES, Vienna, Austria: strain 700284/02

doi: 10.13145/bacdive6881.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria rocourtiae
  • full scientific name: Listeria rocourtiae Leclercq et al. 2010

@ref: 16137

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria rocourtiae

full scientific name: Listeria rocourtiae Leclercq et al. 2010

strain designation: 700284/02

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
29629positive01-02 µm0.4-0.5 µmrod-shapedyes
122245positiverod-shapedno

colony morphology

  • @ref: 16137
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 29629
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16137COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16137BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
37846MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122245CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
16137positivegrowth30mesophilic
29629positivegrowth04-30
29629positiveoptimum30mesophilic
37846positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29629facultative anaerobe
122245facultative anaerobe

spore formation

  • @ref: 29629
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2962917057cellobiose+carbon source
2962928757fructose+carbon source
2962928260galactose+carbon source
2962917234glucose+carbon source
2962917754glycerol+carbon source
2962917306maltose+carbon source
2962929864mannitol+carbon source
2962937684mannose+carbon source
2962928053melibiose+carbon source
29629506227N-acetylglucosamine+carbon source
2962916634raffinose+carbon source
2962926546rhamnose+carbon source
2962933942ribose+carbon source
2962917814salicin+carbon source
2962930911sorbitol+carbon source
2962927082trehalose+carbon source
2962918222xylose+carbon source
2962917632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837116899D-mannitol+builds acid from
6837162345L-rhamnose+builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol+builds acid from
6837117754glycerol+builds acid from
12224517632nitrate+reduction
12224516301nitrite-reduction

metabolite production

  • @ref: 122245
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
16137catalase+1.11.1.6
16137cytochrome-c oxidase-1.9.3.1
29629catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122245oxidase-
122245catalase+1.11.1.6
122245urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122245-+++-+---+++++--++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122245++--++-+/--+++++/-++/-+/-++/--++++++++++-+--+/-+/-+/--+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16137precut lettuceSiezenheim, a village near SalzburgAustriaAUTEurope
62134Pre-cut lettuceSalzburgAustriaAUTEurope2002
122245LettuceInnsbruckAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
161371Risk group (German classification)
1222451Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16137
  • description: Listeria rocourtiae strain CIP 109804 16S ribosomal RNA gene, partial sequence
  • accession: FJ557241
  • length: 1539
  • database: ena
  • NCBI tax ID: 647910

External links

@ref: 16137

culture collection no.: DSM 22097, CIP 109804, CCUG 59857, Allerberger 700284/02, CLIP 2007/00563

straininfo link

  • @ref: 76345
  • straininfo: 370811

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19915117Listeria rocourtiae sp. nov.Leclercq A, Clermont D, Bizet C, Grimont PAD, Le Fleche-Mateos A, Roche SM, Buchrieser C, Cadet-Daniel V, Le Monnier A, Lecuit M, Allerberger FInt J Syst Evol Microbiol10.1099/ijs.0.017376-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Lettuce/*microbiology, Listeria/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny22544790Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond.Lang Halter E, Neuhaus K, Scherer SInt J Syst Evol Microbiol10.1099/ijs.0.036830-02012Araceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Germany, Listeria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16137Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22097)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22097
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29629Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2601928776041
37846Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7588
62134Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59857)https://www.ccug.se/strain?id=59857
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
76345Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370811.1StrainInfo: A central database for resolving microbial strain identifiers
122245Curators of the CIPCollection of Institut Pasteur (CIP 109804)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109804