Strain identifier

BacDive ID: 6873

Type strain: No

Species: Listeria monocytogenes

Strain Designation: 1224.72

Strain history: CIP <- 2011, DSMZ <- NCTC <- P.N. Pini, CPHL, Colindale, UK

NCBI tax ID(s): 1639 (species)

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General

@ref: 6028

BacDive-ID: 6873

DSM-Number: 15675

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Listeria monocytogenes 1224.72 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from soft cheese.

NCBI tax id

  • NCBI tax id: 1639
  • Matching level: species

strain history

@refhistory
6028<- B. Holmes, NCTC <- P. N. Pini, Food Hyg. Lab., CPHL Colindale
121008CIP <- 2011, DSMZ <- NCTC <- P.N. Pini, CPHL, Colindale, UK

doi: 10.13145/bacdive6873.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria monocytogenes
  • full scientific name: Listeria monocytogenes (Murray et al. 1926) Pirie 1940 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium monocytogenes

@ref: 6028

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria monocytogenes

full scientific name: Listeria monocytogenes (Murray et al. 1926) Pirie 1940

strain designation: 1224.72

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
121008positiverod-shapedno

colony morphology

  • @ref: 6028
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6028COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6028BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
41844MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121008CIP Medium 338yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=338
121008CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6028positivegrowth37mesophilic
41844positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121008
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.969

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12100817632nitrate-reduction
12100816301nitrite-reduction
683764853esculin+hydrolysis
6837618333D-arabitol+builds acid from
6837662345L-rhamnose+builds acid from
68376320061methyl alpha-D-glucopyranoside+builds acid from
6837616988D-ribose-builds acid from
6837629042glucose 1-phosphate-builds acid from
6837616443D-tagatose-builds acid from

metabolite production

  • @ref: 121008
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
6028catalase+1.11.1.6
6028cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68376beta-glucosidase+3.2.1.21
121008oxidase-
121008catalase+1.11.1.6
121008urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121008--+-------++----+++-

API LIST

@refDIMESCalpha MANDARLXYLRHAMDGRIBG1PTAGbeta HEM
6028++++-++---+
6028-+++-++---+
6028-++++/-++---
6028-+-+-++---
6028+/-++++/-++----
6028+/-++++/-++----
6028+/-+++-++---

Isolation, sampling and environmental information

isolation

@refsample type
6028soft cheese
121008Food, Soft cheese

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
60282Risk group (German classification)
1210082Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Listeria monocytogenes NCTC11994GCA_900452915contigncbi1639
66792Listeria monocytogenes serotype 4b str. NCTC 11994GCA_002156185contigncbi1344576
66792Listeria monocytogenes serotype 4b str. NCTC 11994 strain NCTC 119941344576.3wgspatric1344576
66792Listeria monocytogenes strain NCTC119941639.4971wgspatric1639

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes59no
motileyes76.478no
gram-positiveyes93.937no
anaerobicno91.854no
halophileno50no
spore-formingno60.087no
thermophileno99.722yes
glucose-utilyes84.773no
aerobicno90.396no
flagellatedno58.029no
glucose-fermentyes60.384no

External links

@ref: 6028

culture collection no.: DSM 15675, NCTC 11994, WDCM 00019, CIP 110274, CECT 4032, LMG 13305

straininfo link

  • @ref: 76338
  • straininfo: 14378

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9248081The effect of high hydrostatic pressure on the activity of intracellular enzymes of Listeria monocytogenes.Simpson RK, Gilmour ALett Appl Microbiol10.1046/j.1472-765x.1997.00168.x1997Hydrostatic Pressure, Listeria monocytogenes/*enzymology
Enzymology9633640Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.Margolles A, Mayo B, de los Reyes-Gavilan CGJ Appl Microbiol10.1046/j.1365-2672.1998.00339.x1998Bacteriophage Typing, Cheese/*microbiology, Electrophoresis, Gel, Pulsed-Field, Humans, Listeria/classification/*genetics, Listeria monocytogenes/classification/*genetics, Listeriosis/etiology, *Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Serotyping, SpainPathogenicity
Pathogenicity10063538Cytotoxic T-cell, delayed type hypersensitive and listeriolysin O responses in experimental bovine listeriosis.Barbuddhe SB, Malik SV, Bhatnagar S, Gupta LKVet Microbiol10.1016/s0378-1135(98)00243-01999Animals, Antibody Formation/immunology, Antigens, Bacterial/blood/*immunology, *Bacterial Toxins, Cattle, Cattle Diseases/*immunology/microbiology, Cytotoxicity Tests, Immunologic/veterinary, Cytotoxicity, Immunologic/immunology, Enzyme-Linked Immunosorbent Assay/veterinary, Heat-Shock Proteins/*blood, Hemolysin Proteins/blood, Hypersensitivity, Delayed/immunology/veterinary, Listeria monocytogenes/*immunology, Listeriosis/immunology/microbiology/*veterinary, Lymphocyte Activation/immunology
Enzymology10375130Pulsed high electric field causes 'all or nothing' membrane damage in Listeria monocytogenes and Salmonella typhimurium, but membrane H+-ATPase is not a primary target.Simpson RK, Whittington R, Earnshaw RG, Russell NJInt J Food Microbiol10.1016/s0168-1605(99)00022-71999Cell Membrane/enzymology/physiology, Cell Membrane Permeability/physiology, Colony Count, Microbial, *Electricity, Fluoresceins/chemistry, Fluorescent Dyes/chemistry, *Food Microbiology, Hydrogen-Ion Concentration, Listeria monocytogenes/enzymology/*growth & development/physiology, Listeriosis/prevention & control, Phosphates/analysis/metabolism, Proton-Translocating ATPases/analysis, Salmonella Food Poisoning/prevention & control, Salmonella typhimurium/enzymology/*growth & development/physiology, Spectrometry, FluorescenceBiotechnology
Biotechnology10791731Mechanism of action of pulsed high electric field (PHEF) on the membranes of food-poisoning bacteria is an 'all-or-nothing' effect.Russell NJ, Colley M, Simpson RK, Trivett AJ, Evans RIInt J Food Microbiol10.1016/s0168-1605(00)00169-02000Cell Membrane, Colony Count, Microbial, Electricity, *Food Handling, *Food Microbiology, Hydrogen-Ion Concentration, Listeria monocytogenes/*growth & development, Salmonella typhimurium/*growth & development
Pathogenicity11556911Inactivation and injury of pressure-resistant strains of Escherichia coli O157 and Listeria monocytogenes in fruit juices.Jordan SL, Pascual C, Bracey E, Mackey BMJ Appl Microbiol10.1046/j.1365-2672.2001.01402.x2001Ascorbic Acid/pharmacology, Beverages/*microbiology, Escherichia coli O157/drug effects/growth & development/*physiology, Food Microbiology, Food Preservation/*methods, Food Preservatives/pharmacology, Fruit/*microbiology, Hydrogen-Ion Concentration, Hydrostatic Pressure, Listeria monocytogenes/drug effects/growth & development/*physiology, Sodium Chloride/pharmacology, Temperature, Time FactorsBiotechnology
Biotechnology11641769Effects of vegetable type, package atmosphere and storage temperature on growth and survival of Escherichia coli O157:H7 and Listeria monocytogenes.Francis GA, O'Beirne DJ Ind Microbiol Biotechnol10.1038/sj.jim.70000942001Carbon Dioxide/analysis, Cold Temperature, Colony Count, Microbial, Escherichia coli O157/*growth & development, *Food Microbiology, *Food Packaging, Humans, Listeria monocytogenes/*growth & development, Oxygen/analysis, Vegetables/*microbiologyPathogenicity
16245862[Effect of high hydrostatic pressure on microbial physiological characteristics].Li ZJ, Xu JXWei Sheng Wu Xue Bao2005Adenosine Triphosphate/analysis, Escherichia coli/cytology/*physiology, Hydrogen-Ion Concentration, *Hydrostatic Pressure, Listeria monocytogenes/cytology/*physiology, Membrane Potentials, Potassium/analysis, Sterilization
16943095Reduction of counts of Listeria monocytogenes in cheese by means of high hydrostatic pressure.Lopez-Pedemonte T, Roig-Sagues A, De Lamo S, Hernandez-Herrero M, Guamis BFood Microbiol10.1016/j.fm.2006.03.0082006Cheese/*microbiology, Colony Count, Microbial, Consumer Product Safety, Food Contamination/*prevention & control, Food Preservation/*methods, Hydrogen-Ion Concentration, *Hydrostatic Pressure, Listeria monocytogenes/*growth & development, Temperature, Time Factors
22064287Influence of oxygen exclusion and temperature on pathogenic bacteria levels and sensory characteristics of packed ostrich steaks throughout refrigerated storage.Gonzalez-Montalvo B, Capita R, Guevara-Franco JA, Prieto M, Alonso-Calleja CMeat Sci10.1016/j.meatsci.2006.10.0252006
Pathogenicity23206192Combined effect of sodium nitrite with high-pressure treatments on the inactivation of Escherichia coli BW25113 and Listeria monocytogenes NCTC 11994.De Alba M, Bravo D, Medina M, Park SF, Mackey BMLett Appl Microbiol10.1111/lam.120312013Anti-Bacterial Agents/pharmacology, Colony Count, Microbial, Escherichia coli/drug effects/*growth & development, *Food Microbiology, Hydrogen-Ion Concentration, Hydrostatic Pressure, Listeria monocytogenes/drug effects/*growth & development, Nitric Oxide/analysis, Sodium Nitrite/*pharmacologyBiotechnology
Metabolism24372804Bacterial inactivation by high-voltage atmospheric cold plasma: influence of process parameters and effects on cell leakage and DNA.Han L, Patil S, Keener KM, Cullen PJ, Bourke PJ Appl Microbiol10.1111/jam.124262014Cell Membrane/metabolism, DNA Damage, Escherichia coli/*growth & development/metabolism, Listeria monocytogenes/*growth & development/metabolism, Microbial Viability, *Plasma Gases, *Sterilization
Biotechnology26408140Behavior of Listeria monocytogenes in Sliced Ready-to-Eat Meat Products Packaged under Vacuum or Modified Atmosphere Conditions.Menendez RA, Rendueles E, Sanz JJ, Capita R, Garcia-Fernandez CJ Food Prot10.4315/0362-028X.JFP-15-1032015Animals, Colony Count, Microbial, Fermentation, Food Contamination/analysis, Food Microbiology, Food Packaging/*methods, Humans, Hydrogen-Ion Concentration, Listeria monocytogenes/*growth & development/isolation & purification, Meat Products/*microbiology, Nitrates/analysis, Sodium Chloride/analysis, Swine, Temperature, VacuumPathogenicity
Phylogeny27077030International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons.Olson ND, Lund SP, Zook JM, Rojas-Cornejo F, Beck B, Foy C, Huggett J, Whale AS, Sui Z, Baoutina A, Dobeson M, Partis L, Morrow JBBiomol Detect Quantif10.1016/j.bdq.2015.01.0042015
31137263Sensitivity of Vegetative Pathogens to High Hydrostatic Pressure Treatment in Phosphate-Buffered Saline and Foods.Patterson MF, Quinn M, Simpson R, Gilmour AJ Food Prot10.4315/0362-028X-58.5.5241995

Reference

@idauthorscataloguedoi/urltitle
6028Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15675)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15675
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41844Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8115
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68376Automatically annotated from API LIST
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76338Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14378.1StrainInfo: A central database for resolving microbial strain identifiers
121008Curators of the CIPCollection of Institut Pasteur (CIP 110274)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110274