Strain identifier

BacDive ID: 6870

Type strain: Yes

Species: Listeria grayi

Strain Designation: Li 2080, 332/64

Strain history: CIP <- 1968, H. Seeliger, Würzburg, Germany: strain SLCC 2080 = strain 332/64

NCBI tax ID(s): 525367 (strain), 1641 (species)

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General

@ref: 8949

BacDive-ID: 6870

DSM-Number: 20601

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive

description: Listeria grayi Li 2080 is a mesophilic, Gram-positive bacterium that was isolated from faeces of chinchilla.

NCBI tax id

NCBI tax idMatching level
1641species
525367strain

strain history

@refhistory
8949<- F. Fiedler <- ATCC <- H. Seeliger, Li 2080 <- H. Errebo Larsen
38051H. Seeliger, Würzburg, Germany: strain SLCC 2080 = strain 332/64 (1968)
67770CIP 68.18 <-- H. P. R. Seeliger; Germany; 332/64 (= SLCC 2080).
119733CIP <- 1968, H. Seeliger, Würzburg, Germany: strain SLCC 2080 = strain 332/64

doi: 10.13145/bacdive6870.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria grayi
  • full scientific name: Listeria grayi Errebo Larsen and Seeliger 1966 (Approved Lists 1980)

@ref: 8949

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria grayi

full scientific name: Listeria grayi Errebo Larsen and Seeliger 1966 emend. Rocourt et al. 1992

strain designation: Li 2080, 332/64

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

  • @ref: 8949
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8949COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8949BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38051MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119733CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
32709CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
32709CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
32709CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8949positivegrowth37mesophilic
38051positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.468

murein

  • @ref: 8949
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4336062968cellulose-hydrolysis
4336017234glucose+fermentation
4336017632nitrate-reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose+builds acid from
6837132528turanose-builds acid from
6837117151xylitol+builds acid from
6837128087glycogen-builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683764853esculin+hydrolysis
6837618333D-arabitol+builds acid from
6837665327D-xylose-builds acid from
6837662345L-rhamnose-builds acid from
6837616988D-ribose+builds acid from
6837629042glucose 1-phosphate-builds acid from
6837616443D-tagatose-builds acid from

metabolite production

  • @ref: 43360
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4336017234glucose+
4336015688acetoin+

enzymes

@refvalueactivityec
8949catalase+1.11.1.6
8949cytochrome-c oxidase-1.9.3.1
68376alpha-mannosidase+3.2.1.24
68376beta-glucosidase+3.2.1.21

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8949-----+/-----+/-+/-+/-+/-----+/--+/-++/-+/-+/-+/-+/-+/-+/-+/---+/----+-++/--+---+----

API LIST

@refDIMESCalpha MANDARLXYLRHAMDGRIBG1PTAGbeta HEM
8949++++--+/-+---
8949++++--++--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8949faeces of chinchilla
67770ChinchillaDenmarkDNKEurope
119733Animal, Chinchilla, fecesDenmarkDNKEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Rodentia (Other)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3208.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_57;97_1999;98_2459;99_3208&stattab=map
  • Last taxonomy: Listeria grayi subclade
  • 16S sequence: NR_026349
  • Sequence Identity:
  • Total samples: 230
  • soil counts: 18
  • aquatic counts: 43
  • animal counts: 157
  • plant counts: 12

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89491Risk group (German classification)
1197331Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Listeria grayi strain H3506 16S ribosomal RNA gene, partial sequenceEU5459791397ena1641
20218Listeria grayi 16S and 23S ribosomal RNA genes, partial sequence, Ile-tRNA and Ala-tRNA genes, complete sequenceU57918548ena525367
20218Listeria grayi subspecies grayi 16S ribosomal RNA gene, partial sequence, 16S/23S ribosomal RNA intergenic spacer region, complete sequence and 23S ribosomal RNA gene, partial sequenceU78983323ena525367
20218L.grayi DNA for 16S ribosomal RNAX985261426ena525367
8949Listeria grayi partial 16S rRNA gene, type strain CECT 931TAJ5155201004ena1641
8949Listeria grayi strain DSM 20601 16S ribosomal RNA, partial sequenceNR_0263491426nuccore1641
67770L.grayi gene for 16S ribosomal RNAX561501493ena1641

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Listeria grayi DSM 20601525367.9wgspatric525367
66792Listeria grayi strain DSM 206011641.11plasmidpatric1641
66792Listeria grayi strain NCTC 108151641.12wgspatric1641
66792Listeria grayi DSM 206012562617157draftimg525367
66792Listeria grayi NCTC 108152916455769draftimg1641
67770Listeria grayi NCTC 10815GCA_900453005contigncbi1641
66792Listeria grayi DSM 20601GCA_000148995scaffoldncbi525367

GC content

@refGC-content
894938.0
6777041

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes68no
gram-positiveyes93.734no
anaerobicno97.927no
halophileyes72.027no
spore-formingno69.594no
glucose-utilyes85.316no
thermophileno99.5yes
motileyes73.466no
aerobicno86.248no
flagellatedno64.841no
glucose-fermentyes69.218yes

External links

@ref: 8949

culture collection no.: DSM 20601, ATCC 19120, CECT 931, JCM 32813, BCRC 14842, CCM 5887, CCUG 4983, CCUG 5118, CIP 105447, CIP 68.18, LMG 16490, NCAIM B.01871, NCTC 10815, CLIP 12518, SLCC 2080

straininfo link

  • @ref: 76335
  • straininfo: 92550

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22544790Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond.Lang Halter E, Neuhaus K, Scherer SInt J Syst Evol Microbiol10.1099/ijs.0.036830-02012Araceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Germany, Listeria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics
23194802The Murein Types of Listeria grayi, Listeria murrayi and Listeria denitrificans.Fiedler F, Seger JSyst Appl Microbiol10.1016/S0723-2020(83)80002-21983

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8949Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20601)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20601
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32709Curators of the CIPCollection of Institut Pasteur (CIP 105447)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105447
38051Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10377
43360H. J. Welshimer, Anne L. Meredith10.1099/00207713-21-1-3Listeria Murrayi sp. N.: a Nitrate-Reducing Mannitol-Fermenting ListeriaIJSB 21: 3-7 1971
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68376Automatically annotated from API LIST
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76335Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92550.1StrainInfo: A central database for resolving microbial strain identifiers
119733Curators of the CIPCollection of Institut Pasteur (CIP 68.18)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2068.18