Strain identifier

BacDive ID: 6867

Type strain: Yes

Species: Brochothrix thermosphacta

Strain Designation: SW 26

Strain history: CIP <- 1988, DSM <- ATCC <- W.L. Sulzbacher: strain SW26, Microbacterium thermosphactum

NCBI tax ID(s): 1121126 (strain), 2756 (species)

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General

@ref: 8602

BacDive-ID: 6867

DSM-Number: 20171

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Brochothrix thermosphacta SW 26 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from fresh pork sausage.

NCBI tax id

NCBI tax idMatching level
2756species
1121126strain

strain history

@refhistory
8602<- ATCC <- W.L. Sulzbacher, SW 26 (Microbacterium thermosphactum)
123514CIP <- 1988, DSM <- ATCC <- W.L. Sulzbacher: strain SW26, Microbacterium thermosphactum

doi: 10.13145/bacdive6867.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Brochothrix
  • species: Brochothrix thermosphacta
  • full scientific name: Brochothrix thermosphacta (McLean and Sulzbacher 1953) Sneath and Jones 1976 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Microbacterium thermosphactum

@ref: 8602

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Brochothrix

species: Brochothrix thermosphacta

full scientific name: Brochothrix thermosphacta (McLean and Sulzbacher 1953) Sneath and Jones 1976

strain designation: SW 26

type strain: yes

Morphology

cell morphology

  • @ref: 123514
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_20171_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20171_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20171_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20171_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20171_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8602CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://mediadive.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
41801MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123514CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123514CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8602positivegrowth30mesophilic
41801positivegrowth25mesophilic
52541positivegrowth22-30

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
52541aerobe
123514facultative anaerobe

compound production

  • @ref: 8602
  • compound: restriction endonuclease MthI

murein

  • @ref: 8602
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12351417632nitrate-reduction
12351416301nitrite-reduction

metabolite production

  • @ref: 123514
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123514oxidase-
123514catalase+1.11.1.6
123514urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123514--++-----+++---++---

Isolation, sampling and environmental information

isolation

@refsample type
8602fresh pork sausage
52541Pork sausage,fresh
123514Food, Pork, sausage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_185;97_201;98_224;99_264&stattab=map
  • Last taxonomy: Brochothrix
  • 16S sequence: AY543023
  • Sequence Identity:
  • Total samples: 40556
  • soil counts: 3704
  • aquatic counts: 5597
  • animal counts: 29797
  • plant counts: 1458

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86021Risk group (German classification)
1235141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brochothrix thermosphacta strain ATCC 11509 16S ribosomal RNA gene, partial sequenceHQ8909421429ena1121126
20218Brochothrix thermosphacta 16S ribosomal RNAM587981552ena2756
20218Brochothrix thermosphacta isolate DSMZ 20171 16S ribosomal RNA gene, partial sequenceAY5430231421ena2756
20218Brochothrix thermosphacta gene for 16S rRNA, partial sequence, strain: NBRC 12167AB6802481473ena1121126
20218B.thermosphacta gene for 16S ribosomal RNAX561551470ena1121126

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brochothrix thermosphacta DSM 20171 = FSL F6-1036GCA_001715655scaffoldncbi1121126
66792Brochothrix thermosphacta DSM 20171 = FSL F6-1036GCA_000620985scaffoldncbi1121126
66792Brochothrix thermosphacta DSM 20171 = FSL F6-1036GCA_000525935contigncbi1121126
66792Brochothrix thermosphacta DSM 201711121126.4wgspatric1121126
66792Brochothrix thermosphacta DSM 20171 = FSL F6-10361121126.7wgspatric1121126
66792Brochothrix thermosphacta DSM 20171 = FSL F6-10361121126.5wgspatric1121126
66792Brochothrix thermosphacta DSM 201712773858004draftimg1121126
66792Brochothrix thermosphacta DSM 201712556921092draftimg1121126

GC content

  • @ref: 8602
  • GC-content: 36.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.986no
gram-positiveyes96.405no
anaerobicno98.395no
aerobicno79.329no
halophileyes78.802no
spore-formingno86.484no
glucose-utilyes89.432no
thermophileno99.508yes
motileno92.007no
glucose-fermentyes78.398no

External links

@ref: 8602

culture collection no.: DSM 20171, ATCC 11509, NCDO 1676, NCIB 10018, WDCM 00071, CCUG 35132, CCM 4769, CIP 103251, NCIMB 10018, CECT 847, NCTC 10822, LMG 17208

straininfo link

  • @ref: 76332
  • straininfo: 8884

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism2995314Regulation of the glucose phosphotransferase system in Brochothrix thermosphacta by membrane energization.Singh SP, Bishop CJ, Vink R, Rogers PJJ Bacteriol10.1128/jb.164.1.367-378.19851985Adenosine Triphosphatases/analysis, Biological Transport, Cell Membrane/ultrastructure, Glucose/*metabolism, Gram-Positive Bacteria/*metabolism, Hydrogen-Ion Concentration, Lactates/metabolism, Lactic Acid, Magnetic Resonance Spectroscopy, Membrane Potentials, Membrane Proteins/analysis, Microscopy, Electron, Phosphoenolpyruvate/metabolism, Phosphorylation, Phosphotransferases/*analysis, Potassium/metabolism
Pathogenicity6438042Growth and end-product formation in fermenter cultures of Brochothrix thermosphacta ATCC 11509T and two psychrotrophic Lactobacillus spp. in different gaseous atmospheres.Blickstad E, Molin GJ Appl Bacteriol10.1111/j.1365-2672.1984.tb01385.x1984Aerobiosis, Anaerobiosis, Bacteria/drug effects/growth & development/*metabolism, Carbon Dioxide/pharmacology, Fermentation, Food Microbiology, Lactates/biosynthesis, Lactic Acid, Lactobacillus/drug effects/growth & development/*metabolism, Oxygen/pharmacology, RefrigerationMetabolism
Biotechnology6660874Growth and end product formation of two psychrotrophic Lactobacillus spp. and Brochothrix thermosphacta ATCC 11509T at different pH values and temperatures.Blickstad EAppl Environ Microbiol10.1128/aem.46.6.1345-1350.19831983Animals, Cell Division, Fermentation, *Food Microbiology, Hydrogen-Ion Concentration, Lactates/biosynthesis, Lactobacillus/*physiology, Meat, Mycobacterium/*physiology, Swine, Temperature
Cultivation10340667The effectiveness of triclosan-incorporated plastic against bacteria on beef surfaces.Cutter CNJ Food Prot10.4315/0362-028x-62.5.4741999Animals, Anti-Infective Agents, Local/*pharmacology, Bacteria/*growth & development, Cattle, Colony Count, Microbial, Culture Media, *Food Packaging, Gram-Negative Bacteria/drug effects/growth & development/isolation & purification, Gram-Positive Bacteria/drug effects/growth & development/isolation & purification, Meat/*microbiology, Microbial Sensitivity Tests, Plastics/*chemistry, Triclosan/*pharmacology, VacuumEnzymology
Phylogeny22544790Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond.Lang Halter E, Neuhaus K, Scherer SInt J Syst Evol Microbiol10.1099/ijs.0.036830-02012Araceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Germany, Listeria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics
Pathogenicity23488765Antimicrobial activity and hydrophobicity of edible whey protein isolate films formulated with nisin and/or glucose oxidase.Murillo-Martinez MM, Tello-Solis SR, Garcia-Sanchez MA, Ponce-Alquicira EJ Food Sci10.1111/1750-3841.120782013Anti-Infective Agents/isolation & purification/*pharmacology, Bacillus/drug effects/growth & development, Enterococcus faecalis/drug effects/growth & development, Escherichia coli/drug effects/growth & development, Food Contamination/prevention & control, Food Microbiology, Food Packaging/*methods, Glucose Oxidase/*pharmacology, *Hydrophobic and Hydrophilic Interactions, Listeria/drug effects/growth & development, Meat/microbiology, Milk Proteins/isolation & purification/*pharmacology, Nisin/*pharmacology, Vegetables/microbiology, Whey ProteinsEnzymology
Enzymology30683371Detection of spoilage associated bacteria using Raman-microspectroscopy combined with multivariate statistical analysis.Klein D, Breuch R, von der Mark S, Wickleder C, Kaul PTalanta10.1016/j.talanta.2018.12.0942018Bacteria/*isolation & purification, *Food Safety, Multivariate Analysis, Spectrum Analysis, Raman/methodsPhylogeny
32887055Differentiation of meat-related microorganisms using paper-based surface-enhanced Raman spectroscopy combined with multivariate statistical analysis.Breuch R, Klein D, Siefke E, Hebel M, Herbert U, Wickleder C, Kaul PTalanta10.1016/j.talanta.2020.1213152020Brochothrix, *Meat, Multivariate Analysis, Salmonella, *Spectrum Analysis, Raman

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8602Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20171)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20171
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41801Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15028
52541Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35132)https://www.ccug.se/strain?id=35132
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76332Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8884.1StrainInfo: A central database for resolving microbial strain identifiers
123514Curators of the CIPCollection of Institut Pasteur (CIP 103251)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103251