Strain identifier

BacDive ID: 6866

Type strain: Yes

Species: Brochothrix campestris

Strain Designation: S3, S3

Strain history: CIP <- 1987, R. Talon, INRA, Ceyrat, France: strain S3

NCBI tax ID(s): 2757 (species)

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General

@ref: 1727

BacDive-ID: 6866

DSM-Number: 4712

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Brochothrix campestris S3 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 2757
  • Matching level: species

strain history

@refhistory
1727<- CIP <- Talon et al., S3
412011987, R. Talon: strain S3
117114CIP <- 1987, R. Talon, INRA, Ceyrat, France: strain S3

doi: 10.13145/bacdive6866.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Brochothrix
  • species: Brochothrix campestris
  • full scientific name: Brochothrix campestris Talon et al. 1988

@ref: 1727

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Brochothrix

species: Brochothrix campestris

full scientific name: Brochothrix campestris Talon et al. 1988

strain designation: S3, S3

type strain: yes

Morphology

cell morphology

  • @ref: 117114
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1727TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41201MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
117114CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1727positivegrowth26mesophilic
41201positivegrowth22psychrophilic
117114positivegrowth15-37

Physiology and metabolism

oxygen tolerance

  • @ref: 117114
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
117114NaClpositivegrowth0-4 %
117114NaClnogrowth6 %
117114NaClnogrowth8 %
117114NaClnogrowth10 %

murein

  • @ref: 1727
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
117114hippurate+hydrolysis606565
117114nitrate-reduction17632
117114nitrite-reduction16301
117114nitrate+respiration17632

metabolite production

  • @ref: 117114
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11711415688acetoin+
11711417234glucose+

enzymes

@refvalueactivityec
117114oxidase-
117114alcohol dehydrogenase-1.1.1.1
117114catalase+1.11.1.6
117114lysine decarboxylase-4.1.1.18
117114ornithine decarboxylase-4.1.1.17
117114urease-3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117114+---+-----+++-+--+---+++++++---+------++------+/---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
1727soil
117114Environment, SoilFranceFRAEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_185;97_201;98_224;99_264&stattab=map
  • Last taxonomy: Brochothrix
  • 16S sequence: AY543038
  • Sequence Identity:
  • Total samples: 40556
  • soil counts: 3704
  • aquatic counts: 5597
  • animal counts: 29797
  • plant counts: 1458

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
17271Risk group (German classification)
1171141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218B.campestris gene for 16S ribosomal RNAX561561480ena2757
20218Brochothrix campestris isolate DSMZ 4712 16S ribosomal RNA gene, partial sequenceAY5430381420ena2757
20218Brochothrix campestris gene for 16S rRNA, partial sequence, strain: NBRC 15547AB6808971473ena2757

GC content

  • @ref: 1727
  • GC-content: 38.0

External links

@ref: 1727

culture collection no.: DSM 4712, ATCC 43754, CIP 102920

straininfo link

  • @ref: 76331
  • straininfo: 92132

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology8357264Brochocin-C, a new bacteriocin produced by Brochothrix campestris.Siragusa GR, Cutter CNAppl Environ Microbiol10.1128/aem.59.7.2326-2328.19931993*Antibiosis, Bacteriocins/*biosynthesis, *Food Microbiology, Gram-Positive Rods/*metabolism, Microbial Sensitivity TestsMetabolism
Pathogenicity9835559Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.McCormick JK, Poon A, Sailer M, Gao Y, Roy KL, McMullen LM, Vederas JC, Stiles ME, Van Belkum MJAppl Environ Microbiol10.1128/AEM.64.12.4757-4766.19981998Amino Acid Sequence, Animals, Bacteriocins/chemistry/*genetics/pharmacology, Base Sequence, Genes, Bacterial, Genetic Complementation Test, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects/*genetics, Meat/microbiology, Microbial Sensitivity Tests, Molecular Sequence Data, Restriction MappingGenetics
Pathogenicity10508055The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-C.Gao Y, van Belkum MJ, Stiles MEAppl Environ Microbiol10.1128/AEM.65.10.4329-4333.19991999Adenosine Triphosphate/metabolism, Bacteriocins/*pharmacology, Cell Membrane/drug effects/physiology, Edetic Acid/pharmacology, Gram-Negative Bacteria/*drug effects, Hydrolysis, Nisin/pharmacologyMetabolism
Metabolism12620816Purification and characterization of brochocin A and brochocin B(10-43), a functional fragment generated by heterologous expression in Carnobacterium piscicola.Garneau S, Ference CA, van Belkum MJ, Stiles ME, Vederas JCAppl Environ Microbiol10.1128/AEM.69.3.1352-1358.20032003Amino Acid Sequence, *Bacteriocins/genetics/isolation & purification/metabolism/pharmacology, Base Sequence, Circular Dichroism, Culture Media, Escherichia coli/genetics/metabolism, Food Preservatives, Lactobacillaceae/drug effects/genetics/*metabolism, Mass Spectrometry/methods, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Analysis, DNAEnzymology
Biotechnology16943083Control of meatborne Listeria monocytogenes and Brochothrix thermosphacta by a bacteriocinogenic Brochothrix campestris ATCC 43754.Greer GG, Dilts BDFood Microbiol10.1016/j.fm.2006.02.0092006Adipose Tissue/microbiology, Animals, Anti-Bacterial Agents, *Antibiosis, Bacteriocins/*biosynthesis, Coculture Techniques, Colony Count, Microbial, Food Microbiology, Gram-Positive Rods/*growth & development/*physiology, Humans, Listeria monocytogenes/*growth & development, Meat/*microbiology/standards, Odorants/analysis, Swine, Taste, Temperature, Time FactorsPathogenicity
Enzymology22738965Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto KBiosci Biotechnol Biochem10.1271/bbb.1109722012Amino Acid Sequence, Animals, Bacteriocins/biosynthesis/genetics/*isolation & purification, Base Sequence, Chromatography, Reverse-Phase, Enterococcus faecium/genetics/*metabolism, Fermentation, Fishes/*microbiology, Lactobacillus/drug effects/metabolism, Microbial Sensitivity Tests, Microbial Viability/drug effects, Molecular Sequence Data, Peptides/genetics/*isolation & purification/metabolism, Recombinant Proteins/biosynthesis/genetics/isolation & purification, Sequence Analysis, DNAPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1727Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4712)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4712
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41201Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76331Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92132.1StrainInfo: A central database for resolving microbial strain identifiers
117114Curators of the CIPCollection of Institut Pasteur (CIP 102920)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102920