Strain identifier
BacDive ID: 6866
Type strain: 
Species: Brochothrix campestris
Strain Designation: S3, S3
Strain history: 1987, R. Talon: strain S3
NCBI tax ID(s): 2757 (species)
version 7.1 (current version)
General
@ref: 1727
BacDive-ID: 6866
DSM-Number: 4712
keywords: 16S sequence, Bacteria, mesophilic
description: Brochothrix campestris S3 is a mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 2757
- Matching level: species
strain history
doi: 10.13145/bacdive6866.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Listeriaceae
- genus: Brochothrix
- species: Brochothrix campestris
- full scientific name: Brochothrix campestris Talon et al. 1988
@ref: 1727
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Listeriaceae
genus: Brochothrix
species: Brochothrix campestris
full scientific name: Brochothrix campestris Talon et al. 1988
strain designation: S3, S3
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1727 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
| 41201 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1727 | positive | growth | 26 | mesophilic |
| 41201 | positive | growth | 22 | psychrophilic |
Physiology and metabolism
murein
- @ref: 1727
- murein short key: A31
- type: A1gamma m-Dpm-direct
Isolation, sampling and environmental information
isolation
- @ref: 1727
- sample type: soil
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_264.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_185;97_201;98_224;99_264&stattab=map
- Last taxonomy: Brochothrix
- 16S sequence: AY543038
- Sequence Identity:
- Total samples: 40556
- soil counts: 3704
- aquatic counts: 5597
- animal counts: 29797
- plant counts: 1458
Safety information
risk assessment
- @ref: 1727
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | B.campestris gene for 16S ribosomal RNA | X56156 | 1480 | ena | 2757 |
| 20218 | Brochothrix campestris isolate DSMZ 4712 16S ribosomal RNA gene, partial sequence | AY543038 | 1420 | ena | 2757 |
| 20218 | Brochothrix campestris gene for 16S rRNA, partial sequence, strain: NBRC 15547 | AB680897 | 1473 | ena | 2757 |
GC content
- @ref: 1727
- GC-content: 38.0
External links
@ref: 1727
culture collection no.: DSM 4712, ATCC 43754, CIP 102920
straininfo link
| @ref | passport |
|---|---|
| 20218 | http://www.straininfo.net/strains/149454 |
| 20218 | http://www.straininfo.net/strains/113098 |
| 20218 | http://www.straininfo.net/strains/113100 |
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Biotechnology | 8357264 | Brochocin-C, a new bacteriocin produced by Brochothrix campestris. | Siragusa GR, Cutter CN | Appl Environ Microbiol | 10.1128/aem.59.7.2326-2328.1993 | 1993 | *Antibiosis, Bacteriocins/*biosynthesis, *Food Microbiology, Gram-Positive Rods/*metabolism, Microbial Sensitivity Tests | Metabolism |
| Pathogenicity | 9835559 | Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754. | McCormick JK, Poon A, Sailer M, Gao Y, Roy KL, McMullen LM, Vederas JC, Stiles ME, Van Belkum MJ | Appl Environ Microbiol | 10.1128/AEM.64.12.4757-4766.1998 | 1998 | Amino Acid Sequence, Animals, Bacteriocins/chemistry/*genetics/pharmacology, Base Sequence, Genes, Bacterial, Genetic Complementation Test, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects/*genetics, Meat/microbiology, Microbial Sensitivity Tests, Molecular Sequence Data, Restriction Mapping | Genetics |
| Pathogenicity | 10508055 | The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-C. | Gao Y, van Belkum MJ, Stiles ME | Appl Environ Microbiol | 10.1128/AEM.65.10.4329-4333.1999 | 1999 | Adenosine Triphosphate/metabolism, Bacteriocins/*pharmacology, Cell Membrane/drug effects/physiology, Edetic Acid/pharmacology, Gram-Negative Bacteria/*drug effects, Hydrolysis, Nisin/pharmacology | Metabolism |
| Metabolism | 12620816 | Purification and characterization of brochocin A and brochocin B(10-43), a functional fragment generated by heterologous expression in Carnobacterium piscicola. | Garneau S, Ference CA, van Belkum MJ, Stiles ME, Vederas JC | Appl Environ Microbiol | 10.1128/AEM.69.3.1352-1358.2003 | 2003 | Amino Acid Sequence, *Bacteriocins/genetics/isolation & purification/metabolism/pharmacology, Base Sequence, Circular Dichroism, Culture Media, Escherichia coli/genetics/metabolism, Food Preservatives, Lactobacillaceae/drug effects/genetics/*metabolism, Mass Spectrometry/methods, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Analysis, DNA | Enzymology |
| Biotechnology | 16943083 | Control of meatborne Listeria monocytogenes and Brochothrix thermosphacta by a bacteriocinogenic Brochothrix campestris ATCC 43754. | Greer GG, Dilts BD | Food Microbiol | 10.1016/j.fm.2006.02.009 | 2006 | Adipose Tissue/microbiology, Animals, Anti-Bacterial Agents, *Antibiosis, Bacteriocins/*biosynthesis, Coculture Techniques, Colony Count, Microbial, Food Microbiology, Gram-Positive Rods/*growth & development/*physiology, Humans, Listeria monocytogenes/*growth & development, Meat/*microbiology/standards, Odorants/analysis, Swine, Taste, Temperature, Time Factors | Pathogenicity |
| Enzymology | 22738965 | Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish. | Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K | Biosci Biotechnol Biochem | 10.1271/bbb.110972 | 2012 | Amino Acid Sequence, Animals, Bacteriocins/biosynthesis/genetics/*isolation & purification, Base Sequence, Chromatography, Reverse-Phase, Enterococcus faecium/genetics/*metabolism, Fermentation, Fishes/*microbiology, Lactobacillus/drug effects/metabolism, Microbial Sensitivity Tests, Microbial Viability/drug effects, Molecular Sequence Data, Peptides/genetics/*isolation & purification/metabolism, Recombinant Proteins/biosynthesis/genetics/isolation & purification, Sequence Analysis, DNA | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1727 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4712) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4712 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 41201 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14660 | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta |