Strain identifier

BacDive ID: 6865

Type strain: Yes

Species: Fructobacillus tropaeoli

Strain Designation: F214-1

Strain history: A. Endo F214-1.

NCBI tax ID(s): 709323 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7 (current version):
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7 (current version)

General

@ref: 16708

BacDive-ID: 6865

DSM-Number: 23246

keywords: Gram-positive, mesophilic, microaerophile, Bacteria, 16S sequence, genome sequence, rod-shaped

description: Fructobacillus tropaeoli F214-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from nasturtium flower Tropaeolum majus.

NCBI tax id

  • NCBI tax id: 709323
  • Matching level: species

strain history

doi: 10.13145/bacdive6865.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Leuconostocaceae
  • genus: Fructobacillus
  • species: Fructobacillus tropaeoli
  • full scientific name: Fructobacillus tropaeoli Endo et al. 2011

@ref: 16708

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Fructobacillus

species: Fructobacillus tropaeoli

full scientific name: Fructobacillus tropaeoli Endo et al. 2011

strain designation: F214-1

type strain: yes

Morphology

cell morphology

  • @ref: 29878
  • gram stain: positive
  • cell length: 3.75 µm
  • cell width: 0.8 µm
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16708DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047)yeshttps://bacmedia.dsmz.de/medium/1047Name: DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047) Composition: Tryptone 20.0 g/l Agar 16.0 g/l Maltose 10.0 g/l Glucose 5.0 g/l Fructose 5.0 g/l Yeast extract 5.0 g/l Potassium Glutamate 2.5 g/l Potassium Aspartate 2.5 g/l Diammonium Citrate 2.0 g/l Dipotassium Phosphate 2.0 g/l Betaine Hydrochloride 2.0 g/l Liver Concentrate 1.0 g/l Magnesium sulfate 0.98 g/l N-Acetylglucosamine 0.5 g/l Manganese Sulfate 0.42 g/l Distilled water
16708MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16708positivegrowth30mesophilic
29878positivegrowth10-45
29878positiveoptimum27.5mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
29878positivegrowth04-08
29878positiveoptimum6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16708microaerophile
29878facultative anaerobe

halophily

  • @ref: 29878
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 2.5 %

observation

  • @ref: 29878
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2987828757fructose+carbon source
2987817234glucose+carbon source
2987829864mannitol+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

@refvalueactivityec
29878acid phosphatase+3.1.3.2
29878alkaline phosphatase+3.1.3.1

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16708------------+-------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
16708nasturtium flower Tropaeolum majusTropaeolum majusWestern Cape, StellenboschSouth AfricaZAFAfrica
67770Nasturtium flowerStellenbosch, Western CapeSouth AfricaZAFAfrica

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Flower

Safety information

risk assessment

  • @ref: 16708
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16708Fructobacillus tropaeoli gene for 16S rRNA, partial sequenceAB5420541519ena709323
67770Fructobacillus tropaeoli JCM 16675 gene for 16S rRNA, partial sequenceLC5198591490ena709323

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fructobacillus tropaeoli F214-1GCA_001047135scaffoldncbi709323
66792Fructobacillus tropaeoli F214-12775506866draftimg709323

GC content

@refGC-contentmethod
1670844
6777044high performance liquid chromatography (HPLC)

External links

@ref: 16708

culture collection no.: DSM 23246, JCM 16675, BCRC 81232

straininfo link

@refpassport
20218http://www.straininfo.net/strains/865199
20218http://www.straininfo.net/strains/865200

literature

  • topic: Phylogeny
  • Pubmed-ID: 20495031
  • title: Fructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower.
  • authors: Endo A, Irisawa T, Futagawa-Endo Y, Sonomoto K, Itoh K, Takano K, Okada S, Dicks LMT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.023838-0
  • year: 2010
  • mesh: Acetic Acid/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fructose/*metabolism, Glucose/metabolism, Lactic Acid/*metabolism, Leuconostocaceae/*classification/genetics/*isolation & purification/metabolism, Mannitol/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, South Africa, Tropaeolum/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16708Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23246)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23246
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2625210.1099/ijs.0.023838-020495031
29878Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126252
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid