Strain identifier

BacDive ID: 6865

Type strain: Yes

Species: Fructobacillus tropaeoli

Strain Designation: F214-1

Strain history: A. Endo F214-1.

NCBI tax ID(s): 709323 (species)

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General

@ref: 16708

BacDive-ID: 6865

DSM-Number: 23246

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Fructobacillus tropaeoli F214-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from nasturtium flower Tropaeolum majus.

NCBI tax id

  • NCBI tax id: 709323
  • Matching level: species

strain history

@refhistory
16708<- A. Endo, Dept. Microbiology, Stellenbosch Univ., South Africa; F214-1
67770A. Endo F214-1.

doi: 10.13145/bacdive6865.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructobacillus
  • species: Fructobacillus tropaeoli
  • full scientific name: Fructobacillus tropaeoli Endo et al. 2011

@ref: 16708

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Fructobacillus

species: Fructobacillus tropaeoli

full scientific name: Fructobacillus tropaeoli Endo et al. 2011

strain designation: F214-1

type strain: yes

Morphology

cell morphology

  • @ref: 29878
  • gram stain: positive
  • cell length: 3.75 µm
  • cell width: 0.8 µm
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16708DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047)yeshttps://mediadive.dsmz.de/medium/1047Name: DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047) Composition: Tryptone 20.0 g/l Agar 16.0 g/l Maltose 10.0 g/l Yeast extract 5.0 g/l Glucose 5.0 g/l Fructose 5.0 g/l Potassium Glutamate 2.5 g/l Potassium Aspartate 2.5 g/l Dipotassium Phosphate 2.0 g/l Betaine Hydrochloride 2.0 g/l Diammonium Citrate 2.0 g/l Liver Concentrate 1.0 g/l Magnesium sulfate 0.98 g/l N-Acetylglucosamine 0.5 g/l Manganese Sulfate 0.42 g/l Distilled water
16708MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16708positivegrowth30mesophilic
29878positivegrowth10-45
29878positiveoptimum27.5mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
29878positivegrowth04-08acidophile
29878positiveoptimum6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16708microaerophile
29878facultative anaerobe

halophily

  • @ref: 29878
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 2.5 %

observation

  • @ref: 29878
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2987828757fructose+carbon source
2987817234glucose+carbon source
2987829864mannitol+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

@refvalueactivityec
29878acid phosphatase+3.1.3.2
29878alkaline phosphatase+3.1.3.1

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16708------------+-------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
16708nasturtium flower Tropaeolum majusTropaeolum majusWestern Cape, StellenboschSouth AfricaZAFAfrica
67770Nasturtium flowerStellenbosch, Western CapeSouth AfricaZAFAfrica

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Flower

taxonmaps

  • @ref: 69479
  • File name: preview.99_2538.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_1366;97_1622;98_1973;99_2538&stattab=map
  • Last taxonomy: Fructobacillus
  • 16S sequence: LC519859
  • Sequence Identity:
  • Total samples: 1983
  • soil counts: 61
  • aquatic counts: 157
  • animal counts: 1617
  • plant counts: 148

Safety information

risk assessment

  • @ref: 16708
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16708Fructobacillus tropaeoli gene for 16S rRNA, partial sequenceAB5420541519ena709323
67770Fructobacillus tropaeoli JCM 16675 gene for 16S rRNA, partial sequenceLC5198591490ena709323

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fructobacillus tropaeoli F214-1GCA_001047135scaffoldncbi709323
66792Fructobacillus tropaeoli F214-12775506866draftimg709323

GC content

@refGC-contentmethod
1670844
6777044high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno97.245yes
motileno97.245yes
flagellatedno98.629yes
flagellatedno98.629yes
gram-positiveyes94.374yes
gram-positiveyes94.374yes
anaerobicno81.667yes
anaerobicno81.667yes
aerobicno97.595yes
aerobicno97.595yes
halophileyes90.743no
halophileyes90.743no
spore-formingno95.374no
spore-formingno95.374no
thermophileno99.068yes
thermophileno99.068yes
glucose-utilyes86.337no
glucose-utilyes86.337no
glucose-fermentyes83.194no
glucose-fermentyes83.194no

External links

@ref: 16708

culture collection no.: DSM 23246, JCM 16675, BCRC 81232

straininfo link

  • @ref: 76330
  • straininfo: 375555

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20495031Fructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower.Endo A, Irisawa T, Futagawa-Endo Y, Sonomoto K, Itoh K, Takano K, Okada S, Dicks LMTInt J Syst Evol Microbiol10.1099/ijs.0.023838-02010Acetic Acid/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fructose/*metabolism, Glucose/metabolism, Lactic Acid/*metabolism, Leuconostocaceae/*classification/genetics/*isolation & purification/metabolism, Mannitol/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, South Africa, Tropaeolum/*microbiologyMetabolism
Phylogeny36260500Fructobacillus cardui sp. nov., isolated from a thistle (Carduus nutans) flower.Gallus MK, Beer I, Ivleva NP, Ehrmann MAInt J Syst Evol Microbiol10.1099/ijsem.0.0055532022RNA, Ribosomal, 16S/genetics, Base Composition, *Carduus/genetics, Catalase/genetics, DNA, Bacterial/genetics, Bacterial Typing Techniques, Sequence Analysis, DNA, Phylogeny, Fatty Acids/chemistry, Flowers, NucleotidesTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16708Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23246)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23246
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29878Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2625228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76330Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID375555.1StrainInfo: A central database for resolving microbial strain identifiers