Strain identifier

BacDive ID: 6864

Type strain: Yes

Species: Fructobacillus durionis

Strain Designation: D-24

Strain history: CIP <- 2005, CCUG <- 2004, LMG <- J. J. Leisner, Copenhagen, Denmark: strain D-24

NCBI tax ID(s): 1121906 (strain), 283737 (species)

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General

@ref: 7900

BacDive-ID: 6864

DSM-Number: 19113

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Fructobacillus durionis D-24 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Tempoyak.

NCBI tax id

NCBI tax idMatching level
283737species
1121906strain

strain history

@refhistory
7900<- CCUG <- M. Vanvanneyt, LMG <- J.J. Leisner, Denmark, D-24
67770NRIC 0663 <-- LMG 22556 <-- J. J. Leisner D-24.
118982CIP <- 2005, CCUG <- 2004, LMG <- J. J. Leisner, Copenhagen, Denmark: strain D-24

doi: 10.13145/bacdive6864.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructobacillus
  • species: Fructobacillus durionis
  • full scientific name: Fructobacillus durionis (Leisner et al. 2005) Endo and Okada 2008

  • synonyms

    • @ref: 20215
    • synonym: Leuconostoc durionis

@ref: 7900

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Fructobacillus

species: Fructobacillus durionis

full scientific name: Fructobacillus durionis (Leisner et al. 2005) Endo and Okada 2008

strain designation: D-24

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31380positiverod-shapedno
69480no96.01
69480positive100
118982positiverod-shapedno

colony morphology

  • @ref: 118982
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7900MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37642MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118982CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
7900positivegrowth30mesophilic
31380positivegrowth5.0-35
31380positiveoptimum20psychrophilic
37642positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

  • @ref: 31380
  • ability: positive
  • type: optimum
  • pH: 3.9
  • PH range: acidophile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7900microaerophile
31380aerobe

spore formation

@refspore formationconfidence
31380no
69481no100
69480no99.999

halophily

  • @ref: 31380
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 8 %

observation

  • @ref: 31380
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3138028757fructose+carbon source
3138024265gluconate+carbon source
3138017234glucose+carbon source
3138017306maltose+carbon source
3138029864mannitol+carbon source
3138033942ribose+carbon source
3138017992sucrose+carbon source
3138027082trehalose+carbon source
11898217632nitrate-reduction
11898216301nitrite-reduction

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
118982oxidase-
118982alcohol dehydrogenase-1.1.1.1
118982catalase-1.11.1.6
118982lysine decarboxylase-4.1.1.18
118982ornithine decarboxylase-4.1.1.17
118982urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118982-+---+----++---+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7900TempoyakMalaysiaMYSAsia
67770Tempoyak (Malaysian acid-fermented condiment made from pulp of the durian fruit)MalaysiaMYSAsia
118982Traditional Malaysian acid-fermented condiment (tempoyak)MalaysiaMYSAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Vegetable (incl. Grains)
#Engineered#Food production#Food
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_40864.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_1658;97_1984;98_29776;99_40864&stattab=map
  • Last taxonomy: Fructobacillus durionis subclade
  • 16S sequence: LC519997
  • Sequence Identity:
  • Total samples: 129
  • soil counts: 4
  • aquatic counts: 3
  • animal counts: 111
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79001Risk group (German classification)
1189821Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Fructobacillus durionis DNA, 16S-23S rRNA intergenic spacer region, strain: LMG 22556AB290444350ena283737
7900Leuconostoc durionis 16S rRNA gene, type strain LMG 22556TAJ7809811502ena283737
67770Fructobacillus durionis JCM 17831 gene for 16S rRNA, partial sequenceLC5199971448ena283737

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fructobacillus durionis strain DSM 19113283737.4wgspatric283737
66792Fructobacillus durionis DSM 191132603880187draftimg283737
67770Fructobacillus durionis DSM 19113GCA_900112405contigncbi283737

GC content

@refGC-contentmethod
790044
6777044high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.861no
gram-positiveyes94.919yes
anaerobicno86.826no
halophileyes94.804yes
spore-formingno96.754no
glucose-utilyes88.075yes
flagellatedno98.604yes
aerobicno96.535no
thermophileno99.175yes
glucose-fermentyes89.558no

External links

@ref: 7900

culture collection no.: DSM 19113, CCUG 49949, CIP 108761, LAB 1679, LMG 22556, JCM 17831, BCRC 81233, CECT 7089, NRIC 0663

straininfo link

  • @ref: 76329
  • straininfo: 96608

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15879266Leuconostoc durionis sp. nov., a heterofermenter with no detectable gas production from glucose.Leisner JJ, Vancanneyt M, Van der Meulen R, Lefebvre K, Engelbeen K, Hoste B, Laursen BG, Bay L, Rusul G, De Vuyst L, Swings JInt J Syst Evol Microbiol10.1099/ijs.0.63434-02005Bacterial Proteins/analysis/isolation & purification, Condiments/*microbiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Electrophoresis, Polyacrylamide Gel, *Food Microbiology, Genes, Bacterial, Genes, rRNA, Glucose/*metabolism, Leuconostoc/chemistry/*classification/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Phylogeny18768629Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov.Endo A, Okada SInt J Syst Evol Microbiol10.1099/ijs.0.65609-02008Acetic Acid/metabolism, Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Ethanol/metabolism, Genes, rRNA, Genotype, Glucose/metabolism, Leuconostoc/*classification/cytology/genetics/metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7900Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19113)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19113
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31380Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127693
37642Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6425
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76329Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID96608.1StrainInfo: A central database for resolving microbial strain identifiers
118982Curators of the CIPCollection of Institut Pasteur (CIP 108761)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108761