Strain identifier
BacDive ID: 6863
Type strain:
Species: Fructobacillus pseudoficulneus
Strain Designation: LC-51, LC51
Strain history: CIP <- 2006, CECT <- 2002, L. Chambel, Lisboa Univ., Lisboa, Portugal: strain LC51
NCBI tax ID(s): 1121907 (strain), 220714 (species)
General
@ref: 5941
BacDive-ID: 6863
DSM-Number: 15468
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, ovoid-shaped
description: Fructobacillus pseudoficulneus LC-51 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from fruit, ripe fig.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1121907 | strain |
220714 | species |
strain history
@ref | history |
---|---|
5941 | <- R. Tenreiro; LC-51 <- L. Chambel |
67770 | NRIC 0698 <-- DSM 15468 <-- R. Tenreiro LC-51 <-- L. Chambel. |
121986 | CIP <- 2006, CECT <- 2002, L. Chambel, Lisboa Univ., Lisboa, Portugal: strain LC51 |
doi: 10.13145/bacdive6863.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Fructobacillus
- species: Fructobacillus pseudoficulneus
- full scientific name: Fructobacillus pseudoficulneus (Chambel et al. 2006) Endo and Okada 2008
synonyms
- @ref: 20215
- synonym: Leuconostoc pseudoficulneum
@ref: 5941
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Leuconostocaceae
genus: Fructobacillus
species: Fructobacillus pseudoficulneus
full scientific name: Fructobacillus pseudoficulneus (Chambel et al. 2006) Endo and Okada 2008
strain designation: LC-51, LC51
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
31686 | positive | 2.2-3 µm | 0.7-1.1 µm | ovoid-shaped | no | |
69480 | no | 96.284 | ||||
69480 | positive | 100 | ||||
121986 | positive | rod-shaped | no |
pigmentation
- @ref: 31686
- production: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5941 | MRS MEDIUM (DSMZ Medium 11) | yes | https://mediadive.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
37507 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
121986 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5941 | positive | growth | 30 | mesophilic |
31686 | positive | growth | 30-37 | mesophilic |
31686 | positive | optimum | 30 | mesophilic |
37507 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
121986 | positive | growth | 15-37 | |
121986 | no | growth | 45 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31686 | positive | growth | 4.8-8.5 | alkaliphile |
31686 | positive | optimum | 6.5-7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
5941 | microaerophile |
31686 | facultative anaerobe |
121986 | obligate aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
31686 | no | |
69481 | no | 100 |
69480 | no | 99.999 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31686 | NaCl | positive | growth | 03-05 % |
31686 | NaCl | positive | optimum | 03-05 % |
observation
- @ref: 31686
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31686 | 29864 | mannitol | + | carbon source |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | - | builds acid from |
68371 | 28066 | gentiobiose | - | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | - | builds acid from |
68371 | 28017 | starch | - | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | - | builds acid from |
68371 | 17992 | sucrose | - | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | - | builds acid from |
68371 | 17306 | maltose | - | builds acid from |
68371 | 17057 | cellobiose | - | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | - | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | - | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 12936 | D-galactose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | - | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
121986 | 17632 | nitrate | - | reduction |
121986 | 16301 | nitrite | - | reduction |
121986 | 17632 | nitrate | + | respiration |
metabolite production
- @ref: 121986
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 121986
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
31686 | acid phosphatase | + | 3.1.3.2 |
31686 | alkaline phosphatase | + | 3.1.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
121986 | oxidase | - | |
121986 | alcohol dehydrogenase | - | 1.1.1.1 |
121986 | catalase | - | 1.11.1.6 |
121986 | lysine decarboxylase | - | 4.1.1.18 |
121986 | ornithine decarboxylase | - | 4.1.1.17 |
121986 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121986 | - | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121986 | - | - | - | - | - | - | - | - | - | - | +/- | + | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | +/- | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
5941 | fruit, ripe fig | Portugal | PRT | Europe | |
67770 | Ripe fig | Portugal | PRT | Europe | Alentejo region |
121986 | Ripe fig | Portugal | PRT | Europe | Alentejo |
isolation source categories
- Cat1: #Host Body-Site
- Cat2: #Plant
- Cat3: #Fruit (Seed)
taxonmaps
- @ref: 69479
- File name: preview.99_2538.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_1366;97_1622;98_1973;99_2538&stattab=map
- Last taxonomy: Fructobacillus
- 16S sequence: LC519998
- Sequence Identity:
- Total samples: 1983
- soil counts: 61
- aquatic counts: 157
- animal counts: 1617
- plant counts: 148
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5941 | 1 | Risk group (German classification) |
121986 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Fructobacillus pseudoficulneus DNA, 16S-23S rRNA intergenic spacer region, strain: DSM 15468 | AB290447 | 363 | ena | 220714 |
31686 | Fructobacillus pseudoficulneus strain LC51 16S ribosomal RNA gene, partial sequence | AY169967 | 1494 | nuccore | 220714 |
67770 | Fructobacillus pseudoficulneus JCM 17832 gene for 16S rRNA, partial sequence | LC519998 | 1481 | ena | 220714 |
5941 | AY16997 | ena |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Fructobacillus pseudoficulneus strain DSM 15468 | 220714.4 | wgs | patric | 220714 |
66792 | Fructobacillus pseudoficulneus strain DSM 15468 | 220714.3 | wgs | patric | 220714 |
66792 | Fructobacillus pseudoficulneus DSM 15468 | 2814123065 | draft | img | 220714 |
66792 | Fructobacillus pseudoficulneus DSM 15468 | 2600254955 | draft | img | 220714 |
67770 | Fructobacillus pseudoficulneus DSM 15468 | GCA_001047115 | scaffold | ncbi | 220714 |
67770 | Fructobacillus pseudoficulneus DSM 15468 | GCA_900108455 | scaffold | ncbi | 220714 |
GC content
@ref | GC-content | method |
---|---|---|
5941 | 44.5 | |
67770 | 44.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 96.904 | yes |
gram-positive | yes | 94.064 | no |
anaerobic | no | 84.321 | no |
aerobic | no | 97.315 | yes |
halophile | yes | 89.952 | no |
spore-forming | no | 96.719 | no |
glucose-util | yes | 84.555 | no |
flagellated | no | 98.656 | no |
thermophile | no | 98.707 | no |
glucose-ferment | yes | 86.621 | no |
External links
@ref: 5941
culture collection no.: DSM 15468, CECT 5759, JCM 17832, BCRC 81234, CIP 109309, LMG 23899, NCIMB 14364, NRIC 0698
straininfo link
- @ref: 76328
- straininfo: 194264
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 16738117 | Leuconostoc pseudoficulneum sp. nov., isolated from a ripe fig. | Chambel L, Chelo IM, Ze-Ze L, Pedro LG, Santos MA, Tenreiro R | Int J Syst Evol Microbiol | 10.1099/ijs.0.64054-0 | 2006 | Base Composition, DNA Primers, DNA, Bacterial/genetics, Fruit/*microbiology, Leuconostoc/*classification/*genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, Species Specificity | Genetics |
Phylogeny | 18768629 | Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov. | Endo A, Okada S | Int J Syst Evol Microbiol | 10.1099/ijs.0.65609-0 | 2008 | Acetic Acid/metabolism, Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Ethanol/metabolism, Genes, rRNA, Genotype, Glucose/metabolism, Leuconostoc/*classification/cytology/genetics/metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
5941 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15468) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15468 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
31686 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 27963 | ||
37507 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7036 | |||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68371 | Automatically annotated from API 50CH acid | ||||||
68382 | Automatically annotated from API zym | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
76328 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID194264.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
121986 | Curators of the CIP | Collection of Institut Pasteur (CIP 109309) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109309 |