Strain identifier

BacDive ID: 6861

Type strain: Yes

Species: Fructobacillus fructosus

Strain Designation: 353

Strain history: CIP <- 1987, DSM <- K.O. Stetter <- Fujikii <- IFO <- Kodama: strain 353

NCBI tax ID(s): 930946 (strain), 1631 (species)

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General

@ref: 8753

BacDive-ID: 6861

DSM-Number: 20349

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Fructobacillus fructosus 353 is an aerobe, mesophilic bacterium that was isolated from flowers.

NCBI tax id

NCBI tax idMatching level
930946strain
1631species

strain history

@refhistory
8753<- K.O. Stetter <- Fujikii <- IFO <- R. Kodama, 353
67770ATCC 13162 <-- IFO 3516 <-- R. Kodama 353.
119471CIP <- 1987, DSM <- K.O. Stetter <- Fujikii <- IFO <- Kodama: strain 353

doi: 10.13145/bacdive6861.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructobacillus
  • species: Fructobacillus fructosus
  • full scientific name: Fructobacillus fructosus (Kodama 1956) Endo and Okada 2008

  • synonyms

    @refsynonym
    20215Leuconostoc fructosum
    20215Lactobacillus fructosus

@ref: 8753

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Fructobacillus

species: Fructobacillus fructosus

full scientific name: Fructobacillus fructosus (Kodama 1956) Endo and Okada 2008

strain designation: 353

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no96
69480100positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8753MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40495MEDIUM 44 - for Lactobacillus fructosusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g);Fructose (10.000 g)
119471CIP Medium 44yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=44

culture temp

@refgrowthtypetemperaturerange
8753positivegrowth30mesophilic
40495positivegrowth30mesophilic
50927positivegrowth37mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50927aerobe
50927microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no100

compound production

@refcompound
8753NAD pyrophosphorylase
8753phosphoribosyl nicotinamide transferase

murein

  • @ref: 8753
  • murein short key: A11.04
  • type: A3alpha L-Lys-L-Ala

observation

  • @ref: 67770
  • observation: Assay of Nicotinamide and fructose

Isolation, sampling and environmental information

isolation

@refsample type
8753flowers
50927Flowers
67770Flowers
119471Plant, Flowers

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Plant
  • Cat3: #Flower

taxonmaps

  • @ref: 69479
  • File name: preview.99_3183.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_1658;97_1984;98_2440;99_3183&stattab=map
  • Last taxonomy: Fructobacillus fructosus subclade
  • 16S sequence: LC062898
  • Sequence Identity:
  • Total samples: 1549
  • soil counts: 38
  • aquatic counts: 49
  • animal counts: 1369
  • plant counts: 93

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87531Risk group (German classification)
1194711Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Leuconostoc fructosus 16S ribosomal RNA gene, partial sequenceAF3607371475ena930946
20218Fructobacillus fructosus gene for 16S rRNA, partial sequence, strain: NBRC 3516AB6800981475ena1631
20218L.fructosus 16S rRNA geneX611401332ena930946
20218Fructobacillus fructosus DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1058AB290446352ena1631
67770Fructobacillus fructosus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1119LC0628981494ena1631

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fructobacillus fructosus KCTC 3544930946.3wgspatric930946
66792Fructobacillus fructosus KCTC 3544 strain DSM 20349930946.7wgspatric930946
66792Fructobacillus fructosus strain NRIC 10581631.5wgspatric1631
66792Fructobacillus fructosus NRIC 10582814123064draftimg1631
66792Fructobacillus fructosus 353, KCTC 3544651324031draftimg930946
66792Fructobacillus fructosus DSM 203492839451520draftimg930946
67770Fructobacillus fructosus KCTC 3544GCA_000185045contigncbi930946
67770Fructobacillus fructosus NRIC 1058GCA_001047095scaffoldncbi1631
67770Fructobacillus fructosus KCTC 3544 DSM 20349GCA_001438695scaffoldncbi930946

GC content

  • @ref: 67770
  • GC-content: 43.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.231no
flagellatedno98.561no
gram-positiveyes92.309no
anaerobicno89.708yes
halophileyes92.816no
spore-formingno96.502no
glucose-utilyes85.559no
aerobicno97.304no
thermophileno99.273yes
glucose-fermentyes87.806no

External links

@ref: 8753

culture collection no.: DSM 20349, ATCC 13162, IFO 3516, NBRC 3516, NCIB 10784, CCUG 32246, NRRL B-2041, LMG 9498, NCIMB 10784, JCM 1119, BCRC 14019, CIP 102985, KCTC 3544, NCFB 2345, NRIC 1058, NCDO 2345

straininfo link

  • @ref: 76326
  • straininfo: 297006

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18768629Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov.Endo A, Okada SInt J Syst Evol Microbiol10.1099/ijs.0.65609-02008Acetic Acid/metabolism, Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Ethanol/metabolism, Genes, rRNA, Genotype, Glucose/metabolism, Leuconostoc/*classification/cytology/genetics/metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidMetabolism
Metabolism30291951Introduction of bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) in Fructobacillus fructosus settled its fructophilic characteristics.Maeno S, Kajikawa A, Dicks L, Endo ARes Microbiol10.1016/j.resmic.2018.09.0042018Acetates/metabolism, Alcohol Dehydrogenase/chemistry/*genetics/*metabolism, Aldehyde Oxidoreductases/chemistry/genetics/*metabolism, Bacterial Proteins/chemistry/*genetics/*metabolism, Fructose/*metabolism, *Gene Expression, Glucose/metabolism, Leuconostoc/*enzymology/genetics, Leuconostocaceae/*genetics/growth & development/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8753Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20349)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20349
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40495Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14731
50927Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32246)https://www.ccug.se/strain?id=32246
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76326Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297006.1StrainInfo: A central database for resolving microbial strain identifiers
119471Curators of the CIPCollection of Institut Pasteur (CIP 102985)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102985