Strain identifier

BacDive ID: 6852

Type strain: Yes

Species: Weissella hellenica

Strain Designation: LV346

Strain history: NCFB 2973 <-- J. Samelis LV346.

NCBI tax ID(s): 46256 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3134

BacDive-ID: 6852

DSM-Number: 7378

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Weissella hellenica LV346 is an aerobe, mesophilic bacterium that was isolated from sausage; naturally-fermented.

NCBI tax id

  • NCBI tax id: 46256
  • Matching level: species

strain history

doi: 10.13145/bacdive6852.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Leuconostocaceae
  • genus: Weissella
  • species: Weissella hellenica
  • full scientific name: Weissella hellenica Collins et al. 1994

@ref: 3134

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Weissella

species: Weissella hellenica

full scientific name: Weissella hellenica Collins et al. 1994

strain designation: LV346

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3134MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40564MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)

culture temp

@refgrowthtypetemperaturerange
3134positivegrowth30mesophilic
40564positivegrowth25
51552positivegrowth20-25
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 51552
  • oxygen tolerance: aerobe

murein

  • @ref: 3134
  • murein short key: A11.09
  • type: A3alpha L-Lys-L-Ala-L-Ala(L-Ser)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51552C14:02.514
    51552C16:025.916
    51552C18:04.318
    51552C16:1 ω7c415.819
    51552C16:1 ω7c/C15:0 ISO 2OH4.115.85
    51552C18:1 ω7c /12t/9t43.917.824
    51552C18:1 ω9c7.117.769
    51552C18:2 ω6,9c/C18:0 ANTE8.317.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
3134----+------+++--------+/------+--++------------+-+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3134sausage; naturally-fermentedGreeceGRCEurope
51552Dry naturally fermented Greek sausage
67770Fermented sausage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

Safety information

risk assessment

  • @ref: 3134
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Weissella hellenica gene for 16S rRNA, partial sequence, strain: NBRC 15553AB6809021496ena46256
20218Weissella hellenica strain NCFB 2973 16S ribosomal RNA gene, partial sequenceS678311527ena46256
20218W.hellenica 16S rRNA geneX959811334ena46256
67770Weissella hellenica gene for 16S ribosomal RNA, partial sequence, strain: JCM 10103LC0962261512ena46256

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Weissella hellenica CCUG 33494GCA_012396435contigncbi46256
66792Weissella hellenica NBRC 15553GCA_006540065contigncbi46256
66792Weissella hellenica NBRC 15553GCA_005405545scaffoldncbi46256
66792Weissella hellenica strain CCUG 3349446256.17wgspatric46256
66792Weissella hellenica strain NBRC 1555346256.14wgspatric46256
66792Weissella hellenica strain NBRC 1555346256.12wgspatric46256
67770Weissella hellenica strain NBRC 15553, whole genome shotgun sequencing projectBJEG00000000ncbi46256
67770Weissella hellenica strain NBRC 15553, whole genome shotgun sequencing projectBJOF00000000ncbi46256
67770Weissella hellenica strain CCUG 33494, whole genome shotgun sequencing projectJAAXPM000000000ncbi46256

GC content

  • @ref: 3134
  • GC-content: 39.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3134

culture collection no.: DSM 7378, ATCC 51523, NCFB 2973, CCUG 33494, LMG 15125, JCM 10103, CECT 7033, CIP 109879, IFO 15553, KCTC 3668, NBRC 15553, NCIMB 702973

straininfo link

@refpassport
20218http://www.straininfo.net/strains/35652
20218http://www.straininfo.net/strains/35650
20218http://www.straininfo.net/strains/35654

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
12501410Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine.Cai Y, Benno Y, Nakase T, Oh TKJ Gen Appl Microbiol10.2323/jgam.44.3111998
25783976Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov.Praet J, Meeus I, Cnockaert M, Houf K, Smagghe G, Vandamme PAntonie Van Leeuwenhoek10.1007/s10482-015-0429-z2015PhylogenyAnimals, Base Composition, Bees/*microbiology, DNA, Bacterial/genetics, Gastrointestinal Tract/microbiology, Lactic Acid/metabolism, Lactobacillales/classification/genetics/*isolation & purification/metabolism, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Weissella/classification/genetics/*isolation & purification/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3134Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7378)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7378
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40564Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7673
51552Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33494)https://www.ccug.se/strain?id=33494
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid