Strain identifier

BacDive ID: 6852

Type strain: Yes

Species: Weissella hellenica

Strain Designation: LV346

Strain history: CIP <- 2008, JCM <- M.D. Collins, Reading lab., UK <- NCFB 2973 <- J. Samelis LV346: strain 10103

NCBI tax ID(s): 46256 (species)

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General

@ref: 3134

BacDive-ID: 6852

DSM-Number: 7378

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, oval-shaped

description: Weissella hellenica LV346 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from sausage; naturally-fermented.

NCBI tax id

  • NCBI tax id: 46256
  • Matching level: species

strain history

@refhistory
3134<- NCFB <- J. Samelis, LV346
67770NCFB 2973 <-- J. Samelis LV346.
119862CIP <- 2008, JCM <- M.D. Collins, Reading lab., UK <- NCFB 2973 <- J. Samelis LV346: strain 10103

doi: 10.13145/bacdive6852.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Weissella
  • species: Weissella hellenica
  • full scientific name: Weissella hellenica Collins et al. 1994

@ref: 3134

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Weissella

species: Weissella hellenica

full scientific name: Weissella hellenica Collins et al. 1994

strain designation: LV346

type strain: yes

Morphology

cell morphology

  • @ref: 119862
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3134MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40564MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119862CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
3134positivegrowth30mesophilic
40564positivegrowth25
51552positivegrowth20-25
67770positivegrowth30mesophilic
119862positivegrowth15-30
119862nogrowth37mesophilic
119862nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51552aerobe
119862facultative anaerobe

murein

  • @ref: 3134
  • murein short key: A11.09
  • type: A3alpha L-Lys-L-Ala-L-Ala(L-Ser)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119862nitrate-reduction17632
119862nitrite-reduction16301
119862nitrate+respiration17632

metabolite tests

  • @ref: 119862
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119862oxidase-
119862alcohol dehydrogenase-1.1.1.1
119862catalase-1.11.1.6
119862lysine decarboxylase-4.1.1.18
119862ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51552C14:02.514
    51552C16:025.916
    51552C18:04.318
    51552C16:1 ω7c415.819
    51552C16:1 ω7c/C15:0 ISO 2OH4.115.85
    51552C18:1 ω7c /12t/9t43.917.824
    51552C18:1 ω9c7.117.769
    51552C18:2 ω6,9c/C18:0 ANTE8.317.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119862-+--------++-+-+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
3134----+------+++--------+/------+--++------------+-+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
3134sausage; naturally-fermentedGreeceGRCEurope
51552Dry naturally fermented Greek sausage
67770Fermented sausage
119862Fermented sausageUnited KingdomGBREuropeReading1993

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_7596.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_155;96_519;97_597;98_686;99_7596&stattab=map
  • Last taxonomy: Weissella hellenica
  • 16S sequence: S67831
  • Sequence Identity:
  • Total samples: 17284
  • soil counts: 1440
  • aquatic counts: 1465
  • animal counts: 13049
  • plant counts: 1330

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31341Risk group (German classification)
1198621Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Weissella hellenica gene for 16S rRNA, partial sequence, strain: NBRC 15553AB6809021496ena46256
20218Weissella hellenica strain NCFB 2973 16S ribosomal RNA gene, partial sequenceS678311527ena46256
20218W.hellenica 16S rRNA geneX959811334ena46256
67770Weissella hellenica gene for 16S ribosomal RNA, partial sequence, strain: JCM 10103LC0962261512ena46256

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Weissella hellenica strain CCUG 3349446256.17wgspatric46256
66792Weissella hellenica strain NBRC 1555346256.14wgspatric46256
66792Weissella hellenica strain NBRC 1555346256.12wgspatric46256
67770Weissella hellenica NBRC 15553GCA_005405545scaffoldncbi46256
67770Weissella hellenica NBRC 15553GCA_006540065contigncbi46256
67770Weissella hellenica CCUG 33494GCA_012396435contigncbi46256

GC content

  • @ref: 3134
  • GC-content: 39.4
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes97.376no
anaerobicno94.499no
halophileyes96.813no
spore-formingno96.787no
glucose-utilyes90.495no
aerobicno90.634yes
flagellatedno98.556no
motileno96.808no
thermophileno99.827yes
glucose-fermentyes90.298no

External links

@ref: 3134

culture collection no.: DSM 7378, ATCC 51523, NCFB 2973, CCUG 33494, LMG 15125, JCM 10103, CECT 7033, CIP 109879, IFO 15553, KCTC 3668, NBRC 15553, NCIMB 702973

straininfo link

  • @ref: 76317
  • straininfo: 8831

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
12501410Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine.Cai Y, Benno Y, Nakase T, Oh TKJ Gen Appl Microbiol10.2323/jgam.44.3111998
25783976Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov.Praet J, Meeus I, Cnockaert M, Houf K, Smagghe G, Vandamme PAntonie Van Leeuwenhoek10.1007/s10482-015-0429-z2015PhylogenyAnimals, Base Composition, Bees/*microbiology, DNA, Bacterial/genetics, Gastrointestinal Tract/microbiology, Lactic Acid/metabolism, Lactobacillales/classification/genetics/*isolation & purification/metabolism, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Weissella/classification/genetics/*isolation & purification/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3134Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7378)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7378
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40564Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7673
51552Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33494)https://www.ccug.se/strain?id=33494
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76317Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8831.1StrainInfo: A central database for resolving microbial strain identifiers
119862Curators of the CIPCollection of Institut Pasteur (CIP 109879)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109879