Strain identifier

BacDive ID: 6851

Type strain: Yes

Species: Weissella paramesenteroides

Strain Designation: R 80, R80

Strain history: CIP <- 1986, DSM, Leuconostoc paramesenteroides <- NCDO <- C.S. Pederson: strain R80

NCBI tax ID(s): 585506 (strain), 1249 (species)

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General

@ref: 8693

BacDive-ID: 6851

DSM-Number: 20288

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Weissella paramesenteroides R 80 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

NCBI tax idMatching level
585506strain
1249species

strain history

@refhistory
8693<- NCDO <- C.S. Pederson, R 80
67770NRIC 1542 <-- ATCC 33313 <-- NCDO 803 <-- C. S. Pederson R80.
121569CIP <- 1986, DSM, Leuconostoc paramesenteroides <- NCDO <- C.S. Pederson: strain R80

doi: 10.13145/bacdive6851.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Weissella
  • species: Weissella paramesenteroides
  • full scientific name: Weissella paramesenteroides (Garvie 1967) Collins et al. 1994

  • synonyms

    • @ref: 20215
    • synonym: Leuconostoc paramesenteroides

@ref: 8693

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Weissella

species: Weissella paramesenteroides

full scientific name: Weissella paramesenteroides (Garvie 1967) Collins et al. 1994

strain designation: R 80, R80

type strain: yes

Morphology

cell morphology

  • @ref: 121569
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8693MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34908MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121569CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8693positivegrowth30mesophilic
34908positivegrowth25mesophilic
49885positivegrowth37mesophilic
67770positivegrowth30mesophilic
121569positivegrowth15-37
121569nogrowth10psychrophilic
121569nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
49885facultative anaerobe
49885microaerophile
121569facultative anaerobe

murein

  • @ref: 8693
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121569nitrate-reduction17632
121569nitrite-reduction16301
121569nitrate+respiration17632

metabolite tests

  • @ref: 121569
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121569oxidase-
121569alcohol dehydrogenase-1.1.1.1
121569catalase-1.11.1.6
121569lysine decarboxylase-4.1.1.18
121569ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49885C14:04.814
    49885C16:037.216
    49885C18:09.218
    49885C16:1 ω7c415.819
    49885C17:0 CYCLO116.888
    49885C18:1 ω7c /12t/9t6.317.824
    49885C18:1 ω9c3.117.769
    49885C18:2 ω6,9c/C18:0 ANTE7.717.724
    49885C19:0 CYCLO ω8c22.518.9
    49885C19:0 CYCLO ω9c4.418.87
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121569-+++------+-++-+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8693----++----++++-------++-----+-+++--+/-----+------+--
121569---++----++++-------++-----+-+++-------+---------

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_5255.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_155;96_519;97_597;98_686;99_5255&stattab=map
  • Last taxonomy: Weissella paramesenteroides
  • 16S sequence: AB023238
  • Sequence Identity:
  • Total samples: 12477
  • soil counts: 1214
  • aquatic counts: 1157
  • animal counts: 9091
  • plant counts: 1015

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86931Risk group (German classification)
1215691Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Weissella paramesenteroides 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY454527592ena1249
20218Weissella paramesenteroides 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY454528495ena1249
20218Weissella paramesenteroides 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY454529399ena1249
20218Leuconostoc paramesenteroides gene for 16S ribosomal RNA, partial sequenceD31672171ena1249
20218W.paramesenteroides 16S ribosomal RNAX959821331ena1249
20218Weissella paramesenteroides DNA for 16S ribosomal RNA, strain NRIC 1542AB0232381473ena1249
67770Weissella paramesenteroides gene for 16S ribosomal RNA, partial sequence, strain: JCM 9890LC0962241518ena1249

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Weissella paramesenteroides ATCC 33313585506.3wgspatric585506
66792Weissella paramesenteroides ATCC 33313645058793draftimg585506
67770Weissella paramesenteroides ATCC 33313GCA_000160575scaffoldncbi585506

GC content

@refGC-contentmethod
869341.5
6777039.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes97.048no
anaerobicno94.535yes
halophileyes96.721no
spore-formingno96.457no
glucose-utilyes88.512no
thermophileno99.747yes
flagellatedno98.468no
aerobicno95.334no
motileno96.537no
glucose-fermentyes92.798no

External links

@ref: 8693

culture collection no.: DSM 20288, ATCC 33313, NCDO 803, CCUG 30068, NRRL B-3471, JCM 9890, BCRC 14006, CECT 4268, CECT 4787, CIP 102421, JCM 6126, KACC 10213, KCTC 3531, LMG 9852, NBRC 109620, NCIMB 13092, NRIC 1542, NRRL B-1186, VTT E-98967, IMET 10704

straininfo link

  • @ref: 76316
  • straininfo: 3523

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity8486542Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides ssp. mesenteroides FR 52.Mathieu F, Suwandhi IS, Rekhif N, Milliere JB, Lefebvre GJ Appl Bacteriol10.1111/j.1365-2672.1993.tb05141.x1993Bacteriocins/*biosynthesis/chemistry/pharmacology, Drug Resistance, Microbial, Endopeptidases, Fermentation, Food Microbiology, Food Preservation, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Hot Temperature, Kinetics, Leuconostoc/drug effects/isolation & purification/*metabolism, Molecular WeightEnzymology
Phylogeny26410078Weissella jogaejeotgali sp. nov., isolated from jogae jeotgal, a traditional Korean fermented seafood.Lee SH, Ku HJ, Ahn MJ, Hong JS, Lee SH, Shin H, Lee KC, Lee JS, Ryu S, Jeon CO, Lee JHInt J Syst Evol Microbiol10.1099/ijsem.0.0006312015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, *Food Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seafood/*microbiology, Sequence Analysis, DNA, Weissella/*classification/genetics/isolation & purificationBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8693Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20288)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20288
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34908Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14106
49885Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30068)https://www.ccug.se/strain?id=30068
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76316Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3523.1StrainInfo: A central database for resolving microbial strain identifiers
121569Curators of the CIPCollection of Institut Pasteur (CIP 102421)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102421