Strain identifier

BacDive ID: 6845

Type strain: Yes

Species: Weissella halotolerans

Strain Designation: G1

Strain history: CIP <- 1987, DSM, Lactobacillus halotolerans <- G. Reuter: strain R61, Lactobacillus viridescens subsp. halotolerans

NCBI tax ID(s): 1123500 (strain), 1615 (species)

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General

@ref: 8465

BacDive-ID: 6845

DSM-Number: 20190

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Weissella halotolerans G1 is an anaerobe, mesophilic bacterium that was isolated from sausage.

NCBI tax id

NCBI tax idMatching level
1123500strain
1615species

strain history

@refhistory
8465<- G. Reuter, G1
67770DSM 20190 <-- G. Reuter 61 ("Lactobacillus viridescens subsp. halotolerans").
119830CIP <- 1987, DSM, Lactobacillus halotolerans <- G. Reuter: strain R61, Lactobacillus viridescens subsp. halotolerans

doi: 10.13145/bacdive6845.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Weissella
  • species: Weissella halotolerans
  • full scientific name: Weissella halotolerans (Kandler et al. 1983 ex Reuter 1970) Collins et al. 1994

  • synonyms

    @refsynonym
    20215Lactobacillus viridescens subsp. halotolerans
    20215Lactobacillus halotolerans

@ref: 8465

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Weissella

species: Weissella halotolerans

full scientific name: Weissella halotolerans (Kandler et al. 1983) Collins et al. 1994

strain designation: G1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no97.062
69480100positive
119830nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8465MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34911MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119830CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8465positivegrowth30mesophilic
34911positivegrowth30mesophilic
51531positivegrowth30mesophilic
67770positivegrowth37mesophilic
119830positivegrowth15-37
119830nogrowth10psychrophilic
119830nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51531anaerobe
51531microaerophile
119830facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

halophily

  • @ref: 119830
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

murein

  • @ref: 8465
  • murein short key: A11.13
  • type: A3alpha L-Lys-L-Ala-L-Ser

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119830nitrate-reduction17632
119830nitrite-reduction16301
119830nitrate+respiration17632

antibiotic resistance

  • @ref: 119830
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119830
  • metabolite: polysaccharides
  • production: no

metabolite tests

  • @ref: 119830
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119830oxidase-
119830alcohol dehydrogenase-1.1.1.1
119830gelatinase-
119830caseinase-3.4.21.50
119830catalase-1.11.1.6
119830lysine decarboxylase-4.1.1.18
119830ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51531C14:02.614
    51531C16:03216
    51531C18:010.318
    51531C16:1 ω7c415.819
    51531C18:1 ω7c /12t/9t11.317.824
    51531C18:1 ω9c13.817.769
    51531C18:2 ω6,9c/C18:0 ANTE26.117.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119830--++------++--+++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119830----+-----+++----+/---++-----+---+--------------++-

Isolation, sampling and environmental information

isolation

@refsample type
8465sausage
51531Sausage
67770Sausage meat
119830Food, Sausage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_7977.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_155;96_3692;97_4533;98_5818;99_7977&stattab=map
  • Last taxonomy: Weissella halotolerans subclade
  • 16S sequence: LC064886
  • Sequence Identity:
  • Total samples: 1422
  • soil counts: 78
  • aquatic counts: 68
  • animal counts: 1224
  • plant counts: 52

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
84651Risk group (German classification)
1198301Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus halotolerans gene for 16S ribosomal RNA, partial sequenceD31691192ena1615
20218Weissella halotolerans gene for 16S rRNA, strain:NRIC 1627AB0229261480ena1615
67770Weissella halotolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 1114LC0648861474ena1615
67770L.halotolerans 16S small subunit ribosomal RNAM230371529ena1615

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Weissella halotolerans DSM 201901123500.3wgspatric1123500
66792Weissella halotolerans DSM 201901123500.6wgspatric1123500
66792Weissella halotolerans DSM 201902522572202draftimg1123500
66792Weissella halotolerans DSM 201902690315969draftimg1123500
67770Weissella halotolerans DSM 20190GCA_000420365scaffoldncbi1123500
67770Weissella halotolerans DSM 20190GCA_001436865scaffoldncbi1123500

GC content

@refGC-content
846544.8
6777043.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.553no
flagellatedno99.163no
gram-positiveyes96.411no
anaerobicno87.161no
aerobicno95.307yes
halophileyes94.199no
spore-formingno97.243no
glucose-utilyes92.752no
thermophileno98.791yes
glucose-fermentyes89.172no

External links

@ref: 8465

culture collection no.: CCUG 33457, LMG 9469, DSM 20190, ATCC 35410, JCM 1114, BCRC 14050, CECT 573, CGMCC 1.2440, CIP 103005, NCIMB 702781, NRIC 1627, VTT E-98965

straininfo link

  • @ref: 76310
  • straininfo: 3302

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8465Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20190)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20190
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34911Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14753
51531Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33457)https://www.ccug.se/strain?id=33457
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76310Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3302.1StrainInfo: A central database for resolving microbial strain identifiers
119830Curators of the CIPCollection of Institut Pasteur (CIP 103005)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103005