Strain identifier
BacDive ID: 6827
Type strain:
Species: Leuconostoc carnosum
Strain Designation: SML40
Strain history: CIP <- 1989, NCFB <- 1988, Shaw: strain SML40
NCBI tax ID(s): 1046593 (strain), 1252 (species)
General
@ref: 2228
BacDive-ID: 6827
DSM-Number: 5576
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped
description: Leuconostoc carnosum SML40 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from vakuum-packaged beef.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1252 | species |
1046593 | strain |
strain history
@ref | history |
---|---|
2228 | <- NCFB <- B.G. Shaw, SML40 |
67770 | NRIC 1722 <-- NCFB 2776 <-- B. G. Shaw SML40. |
117189 | CIP <- 1989, NCFB <- 1988, Shaw: strain SML40 |
doi: 10.13145/bacdive6827.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Leuconostoc
- species: Leuconostoc carnosum
- full scientific name: Leuconostoc carnosum Shaw and Harding 1989
@ref: 2228
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Leuconostocaceae
genus: Leuconostoc
species: Leuconostoc carnosum
full scientific name: Leuconostoc carnosum Shaw and Harding 1989
strain designation: SML40
type strain: yes
Morphology
cell morphology
- @ref: 117189
- gram stain: positive
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2228 | MRS MEDIUM (DSMZ Medium 11) | yes | https://mediadive.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
34246 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
117189 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2228 | positive | growth | 28 | mesophilic |
34246 | positive | growth | 25 | |
49879 | positive | growth | 22 | psychrophilic |
67770 | positive | growth | 30 | mesophilic |
117189 | positive | growth | 10-30 | |
117189 | no | growth | 37 | mesophilic |
117189 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 117189
- oxygen tolerance: facultative anaerobe
murein
- @ref: 2228
- murein short key: A11.05
- type: A3alpha L-Lys-L-Ala2
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | + | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | - | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | + | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
117189 | nitrate | - | reduction | 17632 |
117189 | nitrite | - | reduction | 16301 |
117189 | nitrate | + | respiration | 17632 |
metabolite production
- @ref: 117189
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 117189
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
117189 | oxidase | - | |
117189 | alcohol dehydrogenase | - | 1.1.1.1 |
117189 | catalase | - | 1.11.1.6 |
117189 | lysine decarboxylase | - | 4.1.1.18 |
117189 | ornithine decarboxylase | - | 4.1.1.17 |
117189 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 49879 C14:0 10.7 14 49879 C16:0 31.7 16 49879 C18:0 6.5 18 49879 C16:1 ω7c 13.7 15.819 49879 C18:1 ω7c /12t/9t 17.1 17.824 49879 C18:1 ω9c 13.2 17.769 49879 C18:2 ω6,9c/C18:0 ANTE 7.1 17.724 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117189 | - | + | - | - | - | + | - | - | - | - | + | + | - | - | - | + | - | - | - | - |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2228 | - | - | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | + | + | - | - | +/- | - | - | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
2228 | - | - | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | +/- | - | - | + | + | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - |
117189 | - | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117189 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
2228 | vakuum-packaged beef |
49879 | Meat,vacuum packed |
67770 | Vacuum-packed meats |
117189 | Food, Vacuum packed meat |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Bottled, canned, packed food |
#Engineered | #Food production | #Meat |
taxonmaps
- @ref: 69479
- File name: preview.99_6848.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_36;98_5042;99_6848&stattab=map
- Last taxonomy: Leuconostoc carnosum subclade
- 16S sequence: LC096219
- Sequence Identity:
- Total samples: 5291
- soil counts: 373
- aquatic counts: 682
- animal counts: 4024
- plant counts: 212
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2228 | 1 | Risk group (German classification) |
117189 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | L.carnosum 16S ribosomal RNA | X95977 | 1333 | ena | 1252 |
20218 | Leuconostoc carnosum gene for 16S rRNA, strain:NRIC 1722 | AB022925 | 1450 | ena | 1252 |
20218 | Leuconostoc carnosum DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1722 | AB290436 | 370 | ena | 1252 |
67770 | Leuconostoc carnosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9695 | LC096219 | 1497 | ena | 1252 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Leuconostoc carnosum DSM 5576 | GCA_014207505 | scaffold | ncbi | 1252 |
66792 | Leuconostoc carnosum JCM 9695 | GCA_019655915 | contig | ncbi | 1252 |
66792 | Leuconostoc carnosum KCTC 3525 | GCA_000260375 | contig | ncbi | 1046593 |
66792 | Leuconostoc carnosum KCTC 3525 | 1046593.3 | wgs | patric | 1046593 |
66792 | Leuconostoc carnosum strain DSM 5576 | 1252.25 | wgs | patric | 1252 |
66792 | Leuconostoc carnosum strain JCM 9695 | 1252.31 | wgs | patric | 1252 |
66792 | Leuconostoc carnosum DSM 5576 | 2861435432 | draft | img | 1252 |
66792 | Leuconostoc carnosum KCTC 3525 | 2547132109 | draft | img | 1046593 |
GC content
@ref | GC-content | method |
---|---|---|
2228 | 39.0 | |
67770 | 39 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 96.232 | no |
flagellated | no | 97.905 | no |
gram-positive | yes | 96.807 | no |
anaerobic | no | 93.503 | no |
aerobic | no | 96.101 | no |
halophile | yes | 91.089 | no |
spore-forming | no | 94.634 | no |
thermophile | no | 99.821 | yes |
glucose-util | yes | 88.12 | no |
glucose-ferment | yes | 90.029 | no |
External links
@ref: 2228
culture collection no.: DSM 5576, ATCC 49367, NCFB 2776, CCUG 30059, JCM 9695, CECT 4024, CIP 103319, KCTC 3525, LMG 11498, NCIMB 12898, NRIC 1722
straininfo link
- @ref: 76292
- straininfo: 125691
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 16995536 | Leuconostoc carnosum associated with spoilage of refrigerated whole cooked hams in Greece. | Samelis J, Bjorkroth J, Kakouri A, Rementzis J | J Food Prot | 10.4315/0362-028x-69.9.2268 | 2006 | Aldehyde-Lyases/*analysis, Animals, Consumer Product Safety, Cytochrome P-450 Enzyme System/*analysis, Food Contamination/*analysis, Food Preservation/*methods/standards, Greece, Humans, Lactobacillus/classification/genetics/growth & development, Leuconostoc/classification/genetics/*growth & development, Meat Products/*microbiology, *Phylogeny, Polymorphism, Restriction Fragment Length, Refrigeration, Ribotyping, Species Specificity, Swine | Pathogenicity |
Genetics | 21994929 | Genome sequence of Leuconostoc carnosum KCTC 3525. | Nam SH, Kim A, Choi SH, Kang A, Kim DW, Kim RN, Kim DS, Park HS | J Bacteriol | 10.1128/JB.06028-11 | 2011 | Base Composition, DNA, Bacterial/*chemistry/*genetics, Food Industry/methods, Food Microbiology, *Genome, Bacterial, Leuconostoc/*genetics/isolation & purification, Molecular Sequence Data, Sequence Analysis, DNA/methods | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2228 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5576) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5576 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
34246 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15104 | ||||
49879 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 30059) | https://www.ccug.se/strain?id=30059 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76292 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID125691.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
117189 | Curators of the CIP | Collection of Institut Pasteur (CIP 103319) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103319 |