Strain identifier

BacDive ID: 6824

Type strain: No

Species: Leuconostoc mesenteroides subsp. mesenteroides

Strain history: <- ATCC <- NRRL

NCBI tax ID(s): 33967 (subspecies)

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General

@ref: 8651

BacDive-ID: 6824

DSM-Number: 20240

keywords: Bacteria, mesophilic, polysaccharide production

description: Leuconostoc mesenteroides subsp. mesenteroides DSM 20240 is a mesophilic bacterium that produces polysaccharides.

NCBI tax id

  • NCBI tax id: 33967
  • Matching level: subspecies

strain history

  • @ref: 8651
  • history: <- ATCC <- NRRL

doi: 10.13145/bacdive6824.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Leuconostoc
  • species: Leuconostoc mesenteroides subsp. mesenteroides
  • full scientific name: Leuconostoc mesenteroides subsp. mesenteroides (Tsenkovskii 1878) Garvie 1983

@ref: 8651

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc mesenteroides subsp. mesenteroides

full scientific name: Leuconostoc mesenteroides subsp. mesenteroides (Tsenkovskii 1878) Garvie 1983

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8651COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8651TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
8651MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

  • @ref: 8651
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

compound production

@refcompound
8651dextran, class A
8651polysaccharides dextran

murein

  • @ref: 8651
  • murein short key: A11.20
  • type: A3alpha L-Lys-L-Ser-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371esculin+builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8651----+++----+++-------++--+--+-+++--+----+---------
8651----+++----+++----+--++-+++-+++++--+----+------+-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8651--+/--+-++--+++---+++------++/------

Safety information

risk assessment

  • @ref: 8651
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 8651
  • GC-content: 41.0

External links

@ref: 8651

culture collection no.: DSM 20240, ATCC 10830a, CCM 1886, NCDO 796, NCIB 8710, NRRL B-512 F

literature

  • topic: Enzymology
  • Pubmed-ID: 17109058
  • title: Structural and functional features of fructansucrases present in Leuconostoc mesenteroides ATCC 8293.
  • authors: Olvera C, Centeno-Leija S, Lopez-Munguia A
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-006-9128-0
  • year: 2006
  • mesh: Amino Acid Sequence, Carrier Proteins/metabolism, Computational Biology, Electrophoresis, Polyacrylamide Gel, Fructans/metabolism, Hexosyltransferases/chemistry/genetics/*metabolism, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Lectins/metabolism, Leuconostoc/*enzymology/genetics, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Raffinose/metabolism, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Substrate Specificity, Sucrose/metabolism, Temperature
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
8651Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20240)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20240
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR