Strain identifier

BacDive ID: 6820

Type strain: Yes

Species: Leuconostoc mesenteroides subsp. cremoris

Strain Designation: LF2

Strain history: CIP <- 1987, NCFB <- 1955, E.I. Garvie, Reading, UK: strain LF2

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8751

BacDive-ID: 6820

DSM-Number: 20346

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Leuconostoc mesenteroides subsp. cremoris LF2 is an anaerobe, mesophilic bacterium that was isolated from Hansen's dried starter powder.

NCBI tax id

NCBI tax idMatching level
33965subspecies
586220strain

strain history

@refhistory
8751<- W. Holzapfel <- NCDO <- E.I. Garvie, LF2
67770LMG 6909 <-- NCIB 12008 <-- NCDO 543 <-- E. Garvie LF2.
120901CIP <- 1987, NCFB <- 1955, E.I. Garvie, Reading, UK: strain LF2

doi: 10.13145/bacdive6820.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Leuconostoc
  • species: Leuconostoc mesenteroides subsp. cremoris
  • full scientific name: Leuconostoc mesenteroides subsp. cremoris (Knudsen and Sørensen 1929) Garvie 1983
  • synonyms

    @refsynonym
    20215Betacoccus cremoris
    20215Leuconostoc cremoris

@ref: 8751

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc mesenteroides subsp. cremoris

full scientific name: Leuconostoc mesenteroides subsp. cremoris (Knudsen and Sørensen 1929) Garvie 1983

strain designation: LF2

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no97.587
69480100positive
120901nopositiveoval-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8751MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38110MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120901CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40
120901CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8751positivegrowth30mesophilic
38110positivegrowth25mesophilic
47530positivegrowth30mesophilic
67770positivegrowth30mesophilic
120901positivegrowth22-30
120901nogrowth10psychrophilic
120901nogrowth15psychrophilic
120901nogrowth37mesophilic
120901nogrowth41thermophilic
120901nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
47530anaerobe
120901facultative anaerobe

spore formation

@refspore formationconfidence
69481no98
69480no100

murein

  • @ref: 8751
  • murein short key: A11.20
  • type: A3alpha L-Lys-L-Ser-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120901nitrate-reduction17632
120901nitrite-reduction16301
120901nitrate+respiration17632

antibiotic resistance

  • @ref: 120901
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120901
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 120901
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
120901oxidase-
120901alcohol dehydrogenase-1.1.1.1
120901catalase-1.11.1.6
120901lysine decarboxylase-4.1.1.18
120901ornithine decarboxylase-4.1.1.17
120901urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120901----------++--------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8751----------++----------+---------------------------
8751----------++----------+--------+/-------------------
8751----------++----------+--------+------------------
120901---------++----------+--------+/-------------------

Isolation, sampling and environmental information

isolation

@refsample type
8751Hansen's dried starter powder
47530Hansen's dried starter powder
120901Food, Dried cheese starter powder

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Engineered#Food production#Starter culture

taxonmaps

  • @ref: 69479
  • File name: preview.99_41.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_36;98_39;99_41&stattab=map
  • Last taxonomy: Leuconostoc
  • 16S sequence: M23034
  • Sequence Identity:
  • Total samples: 44756
  • soil counts: 3647
  • aquatic counts: 6333
  • animal counts: 32069
  • plant counts: 2707

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87511Risk group (German classification)
1209011Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218L.cremoris rrn operon, 16S-23S rRNA spacer, tRNA-Ala geneZ75494376ena586220
20218Leuconostoc mesenteroides subsp. cremoris strain CCUG 21965 16S-23S ribosomal RNA intergenic spacer, complete sequenceJQ012780371ena33965
20218L.cremoris 16S small subunit ribosomal RNAM230341493ena33965
20218Leuconostoc mesenteroides strain LMG 6909 16S-23S ribosomal RNA intergenic spacer, partial sequenceHM443965373ena1245
20218Leuconostoc mesenteroides gene for 16S ribosomal RNA, partial sequenceD31670197ena33965
20218Leuconostoc mesenteroides DNA for 16S ribosomal RNA, strain NCFB 543AB0232471453ena33965
67770Leuconostoc mesenteroides subsp. cremoris gene for 16S rRNA, partial sequence, strain: NBRC 107766AB6826631476ena33965
67770Leuconostoc mesenteroides subsp. cremoris gene for 16S ribosomal RNA, partial sequence, strain: JCM 16943LC0970811484ena33965

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leuconostoc mesenteroides subsp. cremoris ATCC 19254586220.3wgspatric586220
66792Leuconostoc mesenteroides subsp. cremoris strain NBRC 10776633965.25wgspatric33965
66792Leuconostoc mesenteroides cremoris ATCC 19254643886109draftimg586220
67770Leuconostoc mesenteroides subsp. cremoris ATCC 19254GCA_000160595scaffoldncbi586220
67770Leuconostoc mesenteroides subsp. cremoris NBRC 107766GCA_007992255contigncbi33965
66792Leuconostoc mesenteroides DSM 20346GCA_029823385scaffoldncbi1245

GC content

@refGC-contentmethod
875143.4
6777038.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
gram-positiveyes96.868no
anaerobicno85.527yes
halophileyes94.575no
spore-formingno96.432no
glucose-utilyes87.8no
thermophileno99.567yes
flagellatedno97.636no
aerobicno97.121no
motileno96.888no
glucose-fermentyes91.088no

External links

@ref: 8751

culture collection no.: DSM 20346, ATCC 19254, CCM 2078, NCDO 543, CCUG 21965, LMG 6909, NCIMB 12008, JCM 16943, CCTM 2731, CIP 103009, IMET 10693, NBRC 107766, NCFB 543, NRRL B-3252, VKM B-1420

straininfo link

  • @ref: 76286
  • straininfo: 46641

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism30712128A genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris.Ozcan E, Selvi SS, Nikerel E, Teusink B, Toksoy Oner E, Cakir TAppl Microbiol Biotechnol10.1007/s00253-019-09630-42019Adenosine Triphosphate/metabolism, Aerobiosis, Carbon/metabolism, Cheese/microbiology, Citrates/metabolism, Fermentation, Food Microbiology, Genes, Bacterial, *Genome, Bacterial, Leuconostoc mesenteroides/*genetics/metabolism, *Metabolic Networks and Pathways, *Odorants, Oxidation-ReductionBiotechnology
Phylogeny32228772Leuconostoc litchii sp. nov., a novel lactic acid bacterium isolated from lychee.Chen YS, Wang LT, Wu YC, Mori K, Tamura T, Chang CH, Chang YC, Wu HC, Yi HH, Wang PYInt J Syst Evol Microbiol10.1099/ijsem.0.0039382020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Fruit/*microbiology, Lactic Acid, Leuconostoc/*classification/isolation & purification, Leuconostoc mesenteroides, Litchi/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TaiwanTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8751Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20346)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20346
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38110Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14759
47530Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 21965)https://www.ccug.se/strain?id=21965
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76286Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46641.1StrainInfo: A central database for resolving microbial strain identifiers
120901Curators of the CIPCollection of Institut Pasteur (CIP 103009)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103009