Strain identifier
BacDive ID: 6820
Type strain:
Species: Leuconostoc mesenteroides subsp. cremoris
Strain Designation: LF2
Strain history: CIP <- 1987, NCFB <- 1955, E.I. Garvie, Reading, UK: strain LF2
NCBI tax ID(s): 586220 (strain), 33965 (subspecies)
General
@ref: 8751
BacDive-ID: 6820
DSM-Number: 20346
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Leuconostoc mesenteroides subsp. cremoris LF2 is an anaerobe, mesophilic bacterium that was isolated from Hansen's dried starter powder.
NCBI tax id
NCBI tax id | Matching level |
---|---|
33965 | subspecies |
586220 | strain |
strain history
@ref | history |
---|---|
8751 | <- W. Holzapfel <- NCDO <- E.I. Garvie, LF2 |
67770 | LMG 6909 <-- NCIB 12008 <-- NCDO 543 <-- E. Garvie LF2. |
120901 | CIP <- 1987, NCFB <- 1955, E.I. Garvie, Reading, UK: strain LF2 |
doi: 10.13145/bacdive6820.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Leuconostoc
- species: Leuconostoc mesenteroides subsp. cremoris
- full scientific name: Leuconostoc mesenteroides subsp. cremoris (Knudsen and Sørensen 1929) Garvie 1983
synonyms
@ref synonym 20215 Betacoccus cremoris 20215 Leuconostoc cremoris
@ref: 8751
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Leuconostocaceae
genus: Leuconostoc
species: Leuconostoc mesenteroides subsp. cremoris
full scientific name: Leuconostoc mesenteroides subsp. cremoris (Knudsen and Sørensen 1929) Garvie 1983
strain designation: LF2
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 97.587 | ||
69480 | 100 | positive | ||
120901 | no | positive | oval-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8751 | MRS MEDIUM (DSMZ Medium 11) | yes | https://mediadive.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
38110 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
120901 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 | |
120901 | CIP Medium 41 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8751 | positive | growth | 30 | mesophilic |
38110 | positive | growth | 25 | mesophilic |
47530 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
120901 | positive | growth | 22-30 | |
120901 | no | growth | 10 | psychrophilic |
120901 | no | growth | 15 | psychrophilic |
120901 | no | growth | 37 | mesophilic |
120901 | no | growth | 41 | thermophilic |
120901 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
47530 | anaerobe |
120901 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 98 |
69480 | no | 100 |
murein
- @ref: 8751
- murein short key: A11.20
- type: A3alpha L-Lys-L-Ser-L-Ala2
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | - | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | - | builds acid from | 16024 |
68371 | D-fructose | - | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
120901 | nitrate | - | reduction | 17632 |
120901 | nitrite | - | reduction | 16301 |
120901 | nitrate | + | respiration | 17632 |
antibiotic resistance
- @ref: 120901
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
- @ref: 120901
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 120901
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
120901 | oxidase | - | |
120901 | alcohol dehydrogenase | - | 1.1.1.1 |
120901 | catalase | - | 1.11.1.6 |
120901 | lysine decarboxylase | - | 4.1.1.18 |
120901 | ornithine decarboxylase | - | 4.1.1.17 |
120901 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120901 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8751 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8751 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8751 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
120901 | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
8751 | Hansen's dried starter powder |
47530 | Hansen's dried starter powder |
120901 | Food, Dried cheese starter powder |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Dairy product |
#Engineered | #Food production | #Starter culture |
taxonmaps
- @ref: 69479
- File name: preview.99_41.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_36;98_39;99_41&stattab=map
- Last taxonomy: Leuconostoc
- 16S sequence: M23034
- Sequence Identity:
- Total samples: 44756
- soil counts: 3647
- aquatic counts: 6333
- animal counts: 32069
- plant counts: 2707
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
8751 | 1 | Risk group (German classification) |
120901 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | L.cremoris rrn operon, 16S-23S rRNA spacer, tRNA-Ala gene | Z75494 | 376 | ena | 586220 |
20218 | Leuconostoc mesenteroides subsp. cremoris strain CCUG 21965 16S-23S ribosomal RNA intergenic spacer, complete sequence | JQ012780 | 371 | ena | 33965 |
20218 | L.cremoris 16S small subunit ribosomal RNA | M23034 | 1493 | ena | 33965 |
20218 | Leuconostoc mesenteroides strain LMG 6909 16S-23S ribosomal RNA intergenic spacer, partial sequence | HM443965 | 373 | ena | 1245 |
20218 | Leuconostoc mesenteroides gene for 16S ribosomal RNA, partial sequence | D31670 | 197 | ena | 33965 |
20218 | Leuconostoc mesenteroides DNA for 16S ribosomal RNA, strain NCFB 543 | AB023247 | 1453 | ena | 33965 |
67770 | Leuconostoc mesenteroides subsp. cremoris gene for 16S rRNA, partial sequence, strain: NBRC 107766 | AB682663 | 1476 | ena | 33965 |
67770 | Leuconostoc mesenteroides subsp. cremoris gene for 16S ribosomal RNA, partial sequence, strain: JCM 16943 | LC097081 | 1484 | ena | 33965 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Leuconostoc mesenteroides subsp. cremoris ATCC 19254 | 586220.3 | wgs | patric | 586220 |
66792 | Leuconostoc mesenteroides subsp. cremoris strain NBRC 107766 | 33965.25 | wgs | patric | 33965 |
66792 | Leuconostoc mesenteroides cremoris ATCC 19254 | 643886109 | draft | img | 586220 |
67770 | Leuconostoc mesenteroides subsp. cremoris ATCC 19254 | GCA_000160595 | scaffold | ncbi | 586220 |
67770 | Leuconostoc mesenteroides subsp. cremoris NBRC 107766 | GCA_007992255 | contig | ncbi | 33965 |
66792 | Leuconostoc mesenteroides DSM 20346 | GCA_029823385 | scaffold | ncbi | 1245 |
GC content
@ref | GC-content | method |
---|---|---|
8751 | 43.4 | |
67770 | 38.6 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 98 | no |
gram-positive | yes | 96.868 | no |
anaerobic | no | 85.527 | yes |
halophile | yes | 94.575 | no |
spore-forming | no | 96.432 | no |
glucose-util | yes | 87.8 | no |
thermophile | no | 99.567 | yes |
flagellated | no | 97.636 | no |
aerobic | no | 97.121 | no |
motile | no | 96.888 | no |
glucose-ferment | yes | 91.088 | no |
External links
@ref: 8751
culture collection no.: DSM 20346, ATCC 19254, CCM 2078, NCDO 543, CCUG 21965, LMG 6909, NCIMB 12008, JCM 16943, CCTM 2731, CIP 103009, IMET 10693, NBRC 107766, NCFB 543, NRRL B-3252, VKM B-1420
straininfo link
- @ref: 76286
- straininfo: 46641
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 30712128 | A genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris. | Ozcan E, Selvi SS, Nikerel E, Teusink B, Toksoy Oner E, Cakir T | Appl Microbiol Biotechnol | 10.1007/s00253-019-09630-4 | 2019 | Adenosine Triphosphate/metabolism, Aerobiosis, Carbon/metabolism, Cheese/microbiology, Citrates/metabolism, Fermentation, Food Microbiology, Genes, Bacterial, *Genome, Bacterial, Leuconostoc mesenteroides/*genetics/metabolism, *Metabolic Networks and Pathways, *Odorants, Oxidation-Reduction | Biotechnology |
Phylogeny | 32228772 | Leuconostoc litchii sp. nov., a novel lactic acid bacterium isolated from lychee. | Chen YS, Wang LT, Wu YC, Mori K, Tamura T, Chang CH, Chang YC, Wu HC, Yi HH, Wang PY | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003938 | 2020 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Fruit/*microbiology, Lactic Acid, Leuconostoc/*classification/isolation & purification, Leuconostoc mesenteroides, Litchi/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8751 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20346) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20346 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
38110 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14759 | ||||
47530 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 21965) | https://www.ccug.se/strain?id=21965 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
76286 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46641.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120901 | Curators of the CIP | Collection of Institut Pasteur (CIP 103009) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103009 |