Strain identifier

BacDive ID: 6814

Type strain: No

Species: Leuconostoc lactis

Strain Designation: LL76

Variant: Isotype of BacDive ID 137658

Strain history: CIP <- 1993, L.M.T. Dicks, Food Ind. Microbiol., Stellenbosch, South Africa, Leuconostoc argentinum: strain LL76 <- F. Dellaglio <- F.C. Gonzales

NCBI tax ID(s): 886872 (strain), 1246 (species)

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General

@ref: 3310

BacDive-ID: 6814

DSM-Number: 8581

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Leuconostoc lactis LL76 is an anaerobe, mesophilic human pathogen that was isolated from raw milk.

NCBI tax id

NCBI tax idMatching level
886872strain
1246species

strain history

@refhistory
3310<- L.M.T. Dicks <- F. Dellaglio <- F.C. Gonzalez et al., LL76
67770DSM 8581 <-- L. M. T. Dicks LL76 <-- F. Dellaglio <-- F. C. Gonzalez.
38487CIP <- 1993, L.M.T. Dicks, Food Ind. Microbiol., Stellenbosch, South Africa, Leuconostoc argentinum: strain LL76 <- F. Dellaglio <- F.C. Gonzales

doi: 10.13145/bacdive6814.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Leuconostoc
  • species: Leuconostoc lactis
  • full scientific name: Leuconostoc lactis Garvie 1960 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Leuconostoc argentinum

@ref: 3310

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc lactis

full scientific name: Leuconostoc lactis Garvie 1960

strain designation: LL76

variant: Isotype of BacDive ID 137658

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.938
69480100positive
38487nopositivecoccus-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3310MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38487CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
3310positivegrowth30mesophilic
54529positivegrowth30mesophilic
54529positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
38487positivegrowth10-41
38487nogrowth5psychrophilic
38487nogrowth44thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
54529anaerobe
54529microaerophile
38487facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

murein

  • @ref: 3310
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
38487esculin-hydrolysis4853
38487nitrate-reduction17632
38487nitrite-reduction16301

antibiotic resistance

  • @ref: 38487
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 38487
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
38487oxidase-
38487alcohol dehydrogenase+1.1.1.1
38487catalase-1.11.1.6
38487lysine decarboxylase-4.1.1.18
38487ornithine decarboxylase-4.1.1.17

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38487---+/-+/-----+/-+/-+/-+/-----+/----+/-+/-+/-+/-+/-+/-+/-+/-+/-+/-+/-------+/---+/--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3310raw milkArgentinaARGMiddle and South America
54529Milk,rawArgentinaARGMiddle and South America
67770Argentine raw milk
38487Food, Raw milkArgentinaARGMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Host#Mammals

taxonmaps

  • @ref: 69479
  • File name: preview.99_2892.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_348;98_2237;99_2892&stattab=map
  • Last taxonomy: Leuconostoc
  • 16S sequence: LC260036
  • Sequence Identity:
  • Total samples: 31080
  • soil counts: 2149
  • aquatic counts: 3705
  • animal counts: 23973
  • plant counts: 1253

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
3310yes, in single cases1Risk group (German classification)
384871Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Leuconostoc argentinum 16S ribosomal RNA gene, partial sequenceAF1754031471ena886872
67770Leuconostoc lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 11052LC2600361485ena1246

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leuconostoc lactis KCTC 3773GCA_000179875contigncbi886872
66792Leuconostoc argentinum KCTC 3773886872.3wgspatric886872
66792Leuconostoc argentinum LL76, KCTC 3773651324066draftimg886872

GC content

@refGC-contentmethod
331041.0thermal denaturation, midpoint method (Tm)
6777040.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.791no
flagellatedno98.003no
gram-positiveyes96.111no
anaerobicno93.403no
halophileyes92.439no
spore-formingno94.919no
thermophileno99.739yes
glucose-utilyes87.609no
aerobicno95.723yes
glucose-fermentyes90.281no

External links

@ref: 3310

culture collection no.: DSM 8581, JCM 11052, CCUG 38887, CECT 5746, CIP 103889, KCTC 3773, LMG 18543

straininfo link

  • @ref: 76280
  • straininfo: 12409

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics20952569Genome sequence of Leuconostoc argentinum KCTC 3773.Nam SH, Choi SH, Kang A, Kim DW, Kim RN, Kim A, Park HSJ Bacteriol10.1128/JB.01148-102010Fermentation, Food Microbiology, Gene Expression Regulation, Bacterial, *Genome, Bacterial, Leuconostoc/*classification/*genetics, Molecular Sequence DataBiotechnology
Genetics25935305Genome sequence analysis of potential probiotic strain Leuconostoc lactis EFEL005 isolated from kimchi.Moon JS, Choi HS, Shin SY, Noh SJ, Jeon CO, Han NSJ Microbiol10.1007/s12275-015-5090-82015Amino Acid Transport Systems/genetics, Bacteriocins/genetics, Base Composition, Base Sequence, Carbohydrate Metabolism/genetics, Carboxy-Lyases/genetics, Cation Transport Proteins/genetics, Fermentation, *Food Microbiology, *Genome, Bacterial, Leuconostoc/*genetics/*isolation & purification, Phylogeny, *Probiotics, *Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3310Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8581)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8581
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38487Curators of the CIPCollection of Institut Pasteur (CIP 103889)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103889
54529Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38887)https://www.ccug.se/strain?id=38887
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76280Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12409.1StrainInfo: A central database for resolving microbial strain identifiers