Strain identifier
BacDive ID: 6788
Type strain: 
Species: Legionella massiliensis
Strain Designation: LegA
Strain history: <- A. Campocasso, URMITE, Univ. Méditerranée, Marseille, France; LegA
NCBI tax ID(s): 1034943 (species)
version 9.1 (current version)
General
@ref: 17777
BacDive-ID: 6788
DSM-Number: 24804
keywords: genome sequence, 16S sequence, Bacteria, Gram-negative, rod-shaped
description: Legionella massiliensis LegA is a Gram-negative, rod-shaped bacterium that was isolated from water from a cooling tower.
NCBI tax id
- NCBI tax id: 1034943
- Matching level: species
strain history
- @ref: 17777
- history: <- A. Campocasso, URMITE, Univ. Méditerranée, Marseille, France; LegA
doi: 10.13145/bacdive6788.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella massiliensis
- full scientific name: Legionella massiliensis Campocasso et al. 2012
@ref: 17777
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella massiliensis
full scientific name: Legionella massiliensis Campocasso et al. 2012
strain designation: LegA
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 30516 | negative | 2 µm | 0.66 µm | rod-shaped | no | |
| 69480 | negative | 98.5 |
colony morphology
- @ref: 17777
- incubation period: 2-3 days
pigmentation
- @ref: 30516
- production: yes
Culture and growth conditions
culture medium
- @ref: 17777
- name: BCYE-AGAR (DSMZ Medium 585)
- growth: yes
- link: https://mediadive.dsmz.de/medium/585
- composition: Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 17777 | positive | growth | 30 |
| 30516 | positive | growth | 28-32 |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69480 | no | 93.446 |
| 69481 | no | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 17777
- sample type: water from a cooling tower
- geographic location: Bouches du Rhone
- country: France
- origin.country: FRA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Cooling tower |
| #Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_1325.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_767;97_895;98_1049;99_1325&stattab=map
- Last taxonomy: Legionella massiliensis subclade
- 16S sequence: JF779685
- Sequence Identity:
- Total samples: 576
- soil counts: 241
- aquatic counts: 279
- animal counts: 48
- plant counts: 8
Safety information
risk assessment
- @ref: 17777
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17777
- description: Legionella massiliensis strain LegA 16S ribosomal RNA gene, partial sequence
- accession: JF779685
- length: 1512
- database: nuccore
- NCBI tax ID: 1034943
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Legionella massiliensis LegA | GCA_000756815 | contig | ncbi | 1034943 |
| 66792 | Legionella massiliensis LegA | 1034943.4 | wgs | patric | 1034943 |
| 66792 | Legionella massiliensis LegA | 2627853956 | draft | img | 1034943 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 98.5 | yes |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 95.65 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 93.446 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 63.796 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 93.907 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 54.196 | no |
External links
@ref: 17777
culture collection no.: DSM 24804, CSUR P146
straininfo link
- @ref: 76258
- straininfo: 396864
literature
- topic: Phylogeny
- Pubmed-ID: 22307511
- title: Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.
- authors: Campocasso A, Boughalmi M, Fournous G, Raoult D, La Scola B
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.037853-0
- year: 2012
- mesh: Amoeba/growth & development, Bacterial Typing Techniques, DNA, Bacterial/genetics, France, Genes, Bacterial, Legionella/*classification/genetics/*isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, *Water Microbiology
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 17777 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24804) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24804 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30516 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26848 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 76258 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID396864.1 | StrainInfo: A central database for resolving microbial strain identifiers |