Strain identifier
BacDive ID: 6787
Type strain:
Species: Legionella cardiaca
Strain Designation: H63
Strain history: N. Cianciotto H63 <-- Northwestern Memorial Hosp., Chicago, IL, USA.
NCBI tax ID(s): 1071983 (species)
General
@ref: 17825
BacDive-ID: 6787
DSM-Number: 25049
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped
description: Legionella cardiaca H63 is a mesophilic, Gram-negative, motile bacterium that was isolated from aortic valve tissue of a patient with native valve endocarditis.
NCBI tax id
- NCBI tax id: 1071983
- Matching level: species
strain history
@ref | history |
---|---|
17825 | <- N. Cianciotto, Northwestern Univ., Chicaco, USA; H63 <- Northwestern Memorial Hospital, Clinical Microbiol. Lab., USA |
67770 | N. Cianciotto H63 <-- Northwestern Memorial Hosp., Chicago, IL, USA. |
doi: 10.13145/bacdive6787.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella cardiaca
- full scientific name: Legionella cardiaca Pearce et al. 2012
@ref: 17825
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella cardiaca
full scientific name: Legionella cardiaca Pearce et al. 2012
strain designation: H63
type strain: yes
Morphology
cell morphology
- @ref: 30583
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 17825
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 17825
- name: BCYE-AGAR (DSMZ Medium 585)
- growth: yes
- link: https://mediadive.dsmz.de/medium/585
- composition: Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17825 | positive | growth | 37 | mesophilic |
30583 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68377 | 15824 | D-fructose | - | builds acid from |
68377 | 17306 | maltose | - | builds acid from |
68377 | 17992 | sucrose | - | builds acid from |
68377 | 18257 | ornithine | - | degradation |
68377 | 16199 | urea | - | hydrolysis |
68377 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
17825 | catalase | + | 1.11.1.6 |
17825 | cytochrome-c oxidase | - | 1.9.3.1 |
30583 | catalase | + | 1.11.1.6 |
30583 | gelatinase | + | |
30583 | cytochrome oxidase | + | 1.9.3.1 |
68377 | tryptophan deaminase | - | 4.1.99.1 |
68377 | beta-galactosidase | - | 3.2.1.23 |
68377 | urease | - | 3.5.1.5 |
68377 | ornithine decarboxylase | - | 4.1.1.17 |
68377 | beta-lactamase | - | 3.5.2.6 |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
17825 | - | - | - | - | - | - | - | + | - | - | + | - | - |
17825 | - | +/- | - | - | - | - | - | - | + | - | - | +/- | - |
17825 | - | - | - | - | - | - | - | - | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
17825 | aortic valve tissue of a patient with native valve endocarditis | IL, Chicago | USA | USA | North America | 41.8818 | -87.6232 |
67770 | Human aortic valve tissue and the causative agent of endocarditis |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | |
#Host Body-Site | #Organ | #Heart |
taxonmaps
- @ref: 69479
- File name: preview.99_14152.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_1036;97_3328;98_4195;99_14152&stattab=map
- Last taxonomy: Legionella cardiaca
- 16S sequence: JF831047
- Sequence Identity:
- Total samples: 145
- soil counts: 23
- aquatic counts: 96
- animal counts: 16
- plant counts: 10
Safety information
risk assessment
- @ref: 17825
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17825
- description: Legionella cardiaca strain H63 16S ribosomal RNA gene, partial sequence
- accession: JF831047
- length: 1423
- database: ena
- NCBI tax ID: 1071983
Genome sequences
- @ref: 66792
- description: Legionella cardiaca H63
- accession: GCA_029026145
- assembly level: complete
- database: ncbi
- NCBI tax ID: 1071983
GC content
@ref | GC-content | method |
---|---|---|
17825 | 41.8 | high performance liquid chromatography (HPLC) |
30583 | 41.8 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 85.124 | no |
flagellated | no | 92.467 | no |
gram-positive | no | 97.62 | yes |
anaerobic | no | 84.277 | no |
aerobic | no | 80.276 | no |
halophile | no | 72.785 | no |
spore-forming | no | 97.047 | no |
glucose-ferment | yes | 70.319 | no |
thermophile | no | 98.095 | yes |
glucose-util | yes | 80.553 | no |
External links
@ref: 17825
culture collection no.: DSM 25049, ATCC BAA 2315, JCM 17854
straininfo link
- @ref: 76257
- straininfo: 401902
literature
- topic: Phylogeny
- Pubmed-ID: 22286905
- title: Legionella cardiaca sp. nov., isolated from a case of native valve endocarditis in a human heart.
- authors: Pearce MM, Theodoropoulos N, Mandel MJ, Brown E, Reed KD, Cianciotto NP
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.039248-0
- year: 2012
- mesh: Animals, Aortic Valve/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Endocarditis/*microbiology, Fatty Acids/analysis, Humans, Legionella/*classification/genetics/*isolation & purification, Mice, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Pathogenicity
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
17825 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25049) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25049 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30583 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26914 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68377 | Automatically annotated from API NH | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76257 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID401902.1 | StrainInfo: A central database for resolving microbial strain identifiers |