Strain identifier

BacDive ID: 6787

Type strain: Yes

Species: Legionella cardiaca

Strain Designation: H63

Strain history: N. Cianciotto H63 <-- Northwestern Memorial Hosp., Chicago, IL, USA.

NCBI tax ID(s): 1071983 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17825

BacDive-ID: 6787

DSM-Number: 25049

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Legionella cardiaca H63 is a mesophilic, Gram-negative, motile bacterium that was isolated from aortic valve tissue of a patient with native valve endocarditis.

NCBI tax id

  • NCBI tax id: 1071983
  • Matching level: species

strain history

@refhistory
17825<- N. Cianciotto, Northwestern Univ., Chicaco, USA; H63 <- Northwestern Memorial Hospital, Clinical Microbiol. Lab., USA
67770N. Cianciotto H63 <-- Northwestern Memorial Hosp., Chicago, IL, USA.

doi: 10.13145/bacdive6787.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella cardiaca
  • full scientific name: Legionella cardiaca Pearce et al. 2012

@ref: 17825

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella cardiaca

full scientific name: Legionella cardiaca Pearce et al. 2012

strain designation: H63

type strain: yes

Morphology

cell morphology

  • @ref: 30583
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 17825
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 17825
  • name: BCYE-AGAR (DSMZ Medium 585)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/585
  • composition: Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base

culture temp

@refgrowthtypetemperaturerange
17825positivegrowth37mesophilic
30583positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
17825catalase+1.11.1.6
17825cytochrome-c oxidase-1.9.3.1
30583catalase+1.11.1.6
30583gelatinase+
30583cytochrome oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
17825-------+--+--
17825-+/-------+--+/--
17825--------+--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
17825aortic valve tissue of a patient with native valve endocarditisIL, ChicagoUSAUSANorth America41.8818-87.6232
67770Human aortic valve tissue and the causative agent of endocarditis

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body-Site#Organ#Heart

taxonmaps

  • @ref: 69479
  • File name: preview.99_14152.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_1036;97_3328;98_4195;99_14152&stattab=map
  • Last taxonomy: Legionella cardiaca
  • 16S sequence: JF831047
  • Sequence Identity:
  • Total samples: 145
  • soil counts: 23
  • aquatic counts: 96
  • animal counts: 16
  • plant counts: 10

Safety information

risk assessment

  • @ref: 17825
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17825
  • description: Legionella cardiaca strain H63 16S ribosomal RNA gene, partial sequence
  • accession: JF831047
  • length: 1423
  • database: ena
  • NCBI tax ID: 1071983

Genome sequences

  • @ref: 66792
  • description: Legionella cardiaca H63
  • accession: GCA_029026145
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 1071983

GC content

@refGC-contentmethod
1782541.8high performance liquid chromatography (HPLC)
3058341.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno85.124no
flagellatedno92.467no
gram-positiveno97.62yes
anaerobicno84.277no
aerobicno80.276no
halophileno72.785no
spore-formingno97.047no
glucose-fermentyes70.319no
thermophileno98.095yes
glucose-utilyes80.553no

External links

@ref: 17825

culture collection no.: DSM 25049, ATCC BAA 2315, JCM 17854

straininfo link

  • @ref: 76257
  • straininfo: 401902

literature

  • topic: Phylogeny
  • Pubmed-ID: 22286905
  • title: Legionella cardiaca sp. nov., isolated from a case of native valve endocarditis in a human heart.
  • authors: Pearce MM, Theodoropoulos N, Mandel MJ, Brown E, Reed KD, Cianciotto NP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.039248-0
  • year: 2012
  • mesh: Animals, Aortic Valve/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Endocarditis/*microbiology, Fatty Acids/analysis, Humans, Legionella/*classification/genetics/*isolation & purification, Mice, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17825Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25049)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25049
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30583Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2691428776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76257Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401902.1StrainInfo: A central database for resolving microbial strain identifiers