Strain identifier

BacDive ID: 678

Type strain: Yes

Species: Priestia flexa

Strain Designation: 131

Strain history: CIP <- 2001, CCUG <- 1991, L.K. Nakamura, NRRL, Peoria, USA <- R.E. Gordon: strain NRS 665 <- B.S. Henry: strain 131

NCBI tax ID(s): 1348625 (strain), 86664 (species)

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General

@ref: 518

BacDive-ID: 678

DSM-Number: 1320

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Priestia flexa 131 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

NCBI tax idMatching level
86664species
1348625strain

strain history

@refhistory
518<- R.E. Gordon, NRS 665 (Bacillus megaterium) <- B.S. Henry, 131
67770DSM 1320 <-- R. E. Gordon NRS 665 <-- B. S. Henry 131.
119399CIP <- 2001, CCUG <- 1991, L.K. Nakamura, NRRL, Peoria, USA <- R.E. Gordon: strain NRS 665 <- B.S. Henry: strain 131

doi: 10.13145/bacdive678.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Priestia
  • species: Priestia flexa
  • full scientific name: Priestia flexa (Priest et al. 1989 ex Batchelor 1919) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus flexus

@ref: 518

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Priestia

species: Priestia flexa

full scientific name: Priestia flexa (Priest et al. 1989) Gupta et al. 2020

strain designation: 131

type strain: yes

Morphology

cell morphology

  • @ref: 119399
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 119399
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37253MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
518NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
119399CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
518positivegrowth30
37253positivegrowth30
67770positivegrowth30

culture pH

  • @ref: 119399
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
119399obligate aerobe
125439obligate aerobe96.8

spore formation

@refspore formationconfidence
119399yes
125439yes90.3
125438yes93.01

halophily

  • @ref: 119399
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11939916947citrate-carbon source
1193994853esculin-hydrolysis
119399606565hippurate-hydrolysis
11939917632nitrate+reduction
11939916301nitrite+reduction
11939917632nitrate-respiration
68371Potassium 5-ketogluconate-builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837112936D-galactose+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117814salicin+builds acid from
683714853esculin-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

  • @ref: 119399
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11939915688acetoin-
11939917234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin+3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
119399oxidase+
119399beta-galactosidase+3.2.1.23
119399alcohol dehydrogenase-1.1.1.1
119399gelatinase+
119399amylase+
119399DNase+
119399caseinase+3.4.21.50
119399catalase+1.11.1.6
119399tween esterase+
119399gamma-glutamyltransferase+2.3.2.2
119399lecithinase+
119399lipase-
119399lysine decarboxylase-4.1.1.18
119399ornithine decarboxylase-4.1.1.17
119399urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119399-+++-+---+++++-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119399+---+----++++/----++--+/-++/---++/-+++++-+/-+++-+/-+----+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119399+++++-+++++++-++++++--+--++----+++++-----+-------++--+-----++------------+-+---++-+---+-+++++++-+-+

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
5181Risk group (German classification)
1193991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus flexus clone BFLEX-1R 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478076578nuccore86664
20218Bacillus flexus gene for 16S ribosomal RNAAB0211851529nuccore86664
20218Bacillus flexus strain IFO15715 16S ribosomal RNA gene, partial sequenceJQ4248881499nuccore86664
20218Bacillus flexus strain IFO 15715 16S ribosomal RNA gene, partial sequenceKC884853501nuccore86664
20218Bacillus flexus strain IFO15715 16S ribosomal RNA gene, partial sequenceKJ5690891480nuccore86664
20218Bacillus flexus gene for 16S rRNA, partial sequence, strain: NBRC 15715AB6809441478nuccore86664
124043Bacillus flexus strain IFO 15715 16S ribosomal RNA gene, partial sequence.KC884849501nuccore182141
124043Bacillus flexus strain IFO 15715 16S ribosomal RNA gene, partial sequence.KC884851501nuccore2743
124043Priestia flexa strain IFO15715.86 16S ribosomal RNA gene, partial sequence.OM9130681294nuccore86664
124043Priestia flexa strain NBRC 15715 16S ribosomal RNA gene, partial sequence.OQ3310481002nuccore86664

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus flexus NBRC 157151348625.4wgspatric1348625
66792Bacillus flexus NBRC 157152731957656draftimg1348625
67770Priestia flexa NBRC 15715GCA_001591565contigncbi1348625

GC content

@refGC-contentmethod
51838.8
6777037-39thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes81.368no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.16no
125438spore-formingspore-formingAbility to form endo- or exosporesyes93.01no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes75.451no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno90.766no
125438motile2+flagellatedAbility to perform flagellated movementyes84.113no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes90.3
125439BacteriaNetmotilityAbility to perform movementyes87.7
125439BacteriaNetgram_stainReaction to gram-stainingvariable80.2
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe96.8

External links

@ref: 518

culture collection no.: DSM 1320, ATCC 49095, CCUG 28525, NRRL NRS-665, CIP 106928, NRS 665, JCM 12301, BCRC 15906, CGMCC 1.3592, HAMBI 2080, IFO 15715, KCTC 3449, LMG 11155, NBRC 15715, NCIMB 13366, VTT E-97063

straininfo link

  • @ref: 70353
  • straininfo: 1119

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166719Bacillus herbersteinensis sp. nov.Wieser M, Worliczek H, Kampfer P, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.63660-02005Austria, Bacillus/chemistry/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Humans, Molecular Sequence Data, *Paintings, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityPathogenicity
Phylogeny16403867Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea.Lim JM, Jeon CO, Lee JC, Ju YJ, Park DJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63701-02006Bacillus/chemistry/*classification/isolation & purification/physiology, Base Composition, Diaminopimelic Acid, Fatty Acids, Korea, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids, Plant Roots/microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Salix/*microbiology, *Soil Microbiology, Species SpecificityGenetics
Phylogeny23973597Identification and discrimination of bacteria using Fourier transform infrared spectroscopy.Maity JP, Kar S, Lin CM, Chen CY, Chang YF, Jean JS, Kulp TRSpectrochim Acta A Mol Biomol Spectrosc10.1016/j.saa.2013.07.0622013Bacillus/*chemistry/classification/isolation & purification, Bacterial Proteins/chemistry, Lipids/chemistry, Sewage/microbiology, Spectroscopy, Fourier Transform Infrared/*methods, Stenotrophomonas maltophilia/*chemistry/classification/isolation & purificationEnzymology
Phylogeny23990650Bacillus paraflexus sp. nov., isolated from compost.Chandna P, Mayilraj S, Kuhad RCInt J Syst Evol Microbiol10.1099/ijs.0.048223-02013Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
518Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1320)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1320
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37253Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19113
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70353Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1119.1StrainInfo: A central database for resolving microbial strain identifiers
119399Curators of the CIPCollection of Institut Pasteur (CIP 106928)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106928
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1