Strain identifier
BacDive ID: 6770
Type strain:
Species: Legionella gresilensis
Strain Designation: Gréoux 11D13
Strain history: CIP <- 2000, J. Freney, E. Herriot Hosp., Lyon, France: strain Gréoux 11D13 <- CNRL
NCBI tax ID(s): 91823 (species)
General
@ref: 15520
BacDive-ID: 6770
DSM-Number: 21218
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped
description: Legionella gresilensis Gréoux 11D13 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from thermal spring.
NCBI tax id
- NCBI tax id: 91823
- Matching level: species
strain history
@ref | history |
---|---|
15520 | <- CIP; Greoux 11 D13 {2008} <- J. Freney |
119738 | CIP <- 2000, J. Freney, E. Herriot Hosp., Lyon, France: strain Gréoux 11D13 <- CNRL |
doi: 10.13145/bacdive6770.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella gresilensis
- full scientific name: Legionella gresilensis Lo Presti et al. 2001
@ref: 15520
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella gresilensis
full scientific name: Legionella gresilensis Lo Presti et al. 2001
strain designation: Gréoux 11D13
type strain: yes
Morphology
cell morphology
- @ref: 119738
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15520 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
34125 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
119738 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15520 | positive | growth | 37 | mesophilic |
34125 | positive | growth | 37 | mesophilic |
56983 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 56983
- oxygen tolerance: microaerophile
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
119738 | oxidase | + | |
119738 | catalase | - | 1.11.1.6 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119738 | - | + | + | + | - | + | + | + | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | isolation date |
---|---|---|---|---|---|---|
15520 | thermal spring | France | FRA | Europe | ||
56983 | Water,thermal spa | France | FRA | Europe | Gréoux | |
119738 | Environment, Thermal spring | France | FRA | Europe | Lyon | 1988 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15520 | 1 | Risk group (German classification) |
119738 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15520
- description: Legionella gresilensis strain Greoux 11 D13 16S ribosomal RNA gene, partial sequence
- accession: AF122883
- length: 1436
- database: ena
- NCBI tax ID: 91823
GC content
- @ref: 15520
- GC-content: 33
- method: high performance liquid chromatography (HPLC)
External links
@ref: 15520
culture collection no.: DSM 21218, ATCC 700509, CIP 106631, CCUG 45920, Greoux 11 D13
straininfo link
- @ref: 76240
- straininfo: 87702
literature
- topic: Phylogeny
- Pubmed-ID: 11760933
- title: Legionella gresilensis sp. nov. and Legionella beliardensis sp. nov., isolated from water in France.
- authors: Lo Presti F, Riffard S, Meugnier H, Reyrolle M, Lasne Y, Grimont PA, Grimont F, Benson RF, Brenner DJ, Steigerwalt AG, Etienne J, Freney J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-51-6-1949
- year: 2001
- mesh: Bacterial Proteins/analysis/chemistry, DNA, Ribosomal Spacer/genetics, Fatty Acids/analysis, France, Legionella/*classification/*genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Ubiquinone/analysis, *Water Microbiology
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
15520 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21218) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21218 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
34125 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18783 | ||
56983 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 45920) | https://www.ccug.se/strain?id=45920 | |
68382 | Automatically annotated from API zym | |||
76240 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID87702.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
119738 | Curators of the CIP | Collection of Institut Pasteur (CIP 106631) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106631 |