Strain identifier
BacDive ID: 6766
Type strain:
Species: Legionella waltersii
Strain Designation: 2074-AUS-E
Strain history: CIP <- 1996, R.F. Benson, CDC: strain 2074-AUS-E <- R.P. Walters
NCBI tax ID(s): 66969 (species)
General
@ref: 15903
BacDive-ID: 6766
DSM-Number: 21908
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped
description: Legionella waltersii 2074-AUS-E is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from drinking water distribution system.
NCBI tax id
- NCBI tax id: 66969
- Matching level: species
strain history
@ref | history |
---|---|
15903 | <- CCUG <- CIP <- R. F. Benson, CDC; 2074-AUS-E <- R. P. Walters |
123904 | CIP <- 1996, R.F. Benson, CDC: strain 2074-AUS-E <- R.P. Walters |
doi: 10.13145/bacdive6766.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella waltersii
- full scientific name: Legionella waltersii Benson et al. 1996
@ref: 15903
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella waltersii
full scientific name: Legionella waltersii Benson et al. 1996
strain designation: 2074-AUS-E
type strain: yes
Morphology
cell morphology
- @ref: 123904
- gram stain: negative
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 15903
- incubation period: 3-7 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15903 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
39149 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
123904 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 | |
123904 | CIP Medium 748 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=748 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15903 | positive | growth | 30 | mesophilic |
39149 | positive | growth | 37 | mesophilic |
56546 | positive | growth | 37 | mesophilic |
123904 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
15903 | microaerophile |
56546 | microaerophile |
enzymes
@ref | value | activity | ec |
---|---|---|---|
15903 | catalase | + | 1.11.1.6 |
15903 | cytochrome-c oxidase | - | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
123904 | oxidase | + | |
123904 | catalase | + | 1.11.1.6 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123904 | - | + | - | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15903 | drinking water distribution system | Adelaide | Australia | AUS | Australia and Oceania |
56546 | Drinking water distribution system | Adelaide | Australia | AUS | Australia and Oceania |
123904 | Environment, Drinking water distribution system | Adelaide | Australia | AUS | Australia and Oceania |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Freshwater
taxonmaps
- @ref: 69479
- File name: preview.99_6757.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_73;98_4984;99_6757&stattab=map
- Last taxonomy: Legionella waltersii
- 16S sequence: AF122886
- Sequence Identity:
- Total samples: 305
- soil counts: 96
- aquatic counts: 141
- animal counts: 45
- plant counts: 23
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15903 | 1 | Risk group (German classification) |
123904 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15903
- description: Legionella waltersii strain 2074-AUS-E 16S ribosomal RNA gene, partial sequence
- accession: AF122886
- length: 1447
- database: ena
- NCBI tax ID: 66969
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Legionella waltersii 2074-AUS-E | GCA_900640055 | contig | ncbi | 66969 |
66792 | Legionella waltersii ATCC 51914 | GCA_001468085 | scaffold | ncbi | 66969 |
66792 | Legionella waltersii strain ATCC 51914 | 66969.6 | wgs | patric | 66969 |
66792 | Legionella waltersii ATCC 51914 | 2663762636 | draft | img | 66969 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 79.485 | no |
gram-positive | no | 97.836 | no |
anaerobic | no | 98.531 | yes |
aerobic | no | 87.659 | yes |
halophile | no | 87.772 | no |
spore-forming | no | 92.74 | no |
glucose-util | yes | 69.462 | no |
motile | yes | 76.903 | no |
thermophile | no | 97.767 | no |
glucose-ferment | no | 85.947 | no |
External links
@ref: 15903
culture collection no.: DSM 21908, ATCC 51914, CCUG 44924, CIP 104965
straininfo link
- @ref: 76236
- straininfo: 361673
literature
- topic: Phylogeny
- Pubmed-ID: 8782669
- title: Legionella waltersii sp. nov. and an unnamed Legionella genomospecies isolated from water in Australia.
- authors: Benson RF, Thacker WL, Daneshvar MI, Brenner DJ
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-46-3-631
- year: 1996
- mesh: Agglutination Tests, Australia, DNA, Bacterial/classification, Fatty Acids/metabolism, Legionella/*classification/genetics/isolation & purification/metabolism, Quinones/metabolism, *Water Microbiology, Water Supply
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15903 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21908) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21908 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
39149 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16931 | ||||
56546 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 44924) | https://www.ccug.se/strain?id=44924 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76236 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID361673.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123904 | Curators of the CIP | Collection of Institut Pasteur (CIP 104965) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104965 |