Strain identifier

BacDive ID: 6766

Type strain: Yes

Species: Legionella waltersii

Strain Designation: 2074-AUS-E

Strain history: CIP <- 1996, R.F. Benson, CDC: strain 2074-AUS-E <- R.P. Walters

NCBI tax ID(s): 66969 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15903

BacDive-ID: 6766

DSM-Number: 21908

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella waltersii 2074-AUS-E is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from drinking water distribution system.

NCBI tax id

  • NCBI tax id: 66969
  • Matching level: species

strain history

@refhistory
15903<- CCUG <- CIP <- R. F. Benson, CDC; 2074-AUS-E <- R. P. Walters
123904CIP <- 1996, R.F. Benson, CDC: strain 2074-AUS-E <- R.P. Walters

doi: 10.13145/bacdive6766.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella waltersii
  • full scientific name: Legionella waltersii Benson et al. 1996

@ref: 15903

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella waltersii

full scientific name: Legionella waltersii Benson et al. 1996

strain designation: 2074-AUS-E

type strain: yes

Morphology

cell morphology

  • @ref: 123904
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 15903
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15903BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
39149MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123904CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23
123904CIP Medium 748yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=748

culture temp

@refgrowthtypetemperaturerange
15903positivegrowth30mesophilic
39149positivegrowth37mesophilic
56546positivegrowth37mesophilic
123904positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15903microaerophile
56546microaerophile

enzymes

@refvalueactivityec
15903catalase+1.11.1.6
15903cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
123904oxidase+
123904catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123904-+-+-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15903drinking water distribution systemAdelaideAustraliaAUSAustralia and Oceania
56546Drinking water distribution systemAdelaideAustraliaAUSAustralia and Oceania
123904Environment, Drinking water distribution systemAdelaideAustraliaAUSAustralia and Oceania

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_6757.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_73;98_4984;99_6757&stattab=map
  • Last taxonomy: Legionella waltersii
  • 16S sequence: AF122886
  • Sequence Identity:
  • Total samples: 305
  • soil counts: 96
  • aquatic counts: 141
  • animal counts: 45
  • plant counts: 23

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
159031Risk group (German classification)
1239042Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15903
  • description: Legionella waltersii strain 2074-AUS-E 16S ribosomal RNA gene, partial sequence
  • accession: AF122886
  • length: 1447
  • database: ena
  • NCBI tax ID: 66969

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella waltersii 2074-AUS-EGCA_900640055contigncbi66969
66792Legionella waltersii ATCC 51914GCA_001468085scaffoldncbi66969
66792Legionella waltersii strain ATCC 5191466969.6wgspatric66969
66792Legionella waltersii ATCC 519142663762636draftimg66969

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno79.485no
gram-positiveno97.836no
anaerobicno98.531yes
aerobicno87.659yes
halophileno87.772no
spore-formingno92.74no
glucose-utilyes69.462no
motileyes76.903no
thermophileno97.767no
glucose-fermentno85.947no

External links

@ref: 15903

culture collection no.: DSM 21908, ATCC 51914, CCUG 44924, CIP 104965

straininfo link

  • @ref: 76236
  • straininfo: 361673

literature

  • topic: Phylogeny
  • Pubmed-ID: 8782669
  • title: Legionella waltersii sp. nov. and an unnamed Legionella genomospecies isolated from water in Australia.
  • authors: Benson RF, Thacker WL, Daneshvar MI, Brenner DJ
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-46-3-631
  • year: 1996
  • mesh: Agglutination Tests, Australia, DNA, Bacterial/classification, Fatty Acids/metabolism, Legionella/*classification/genetics/isolation & purification/metabolism, Quinones/metabolism, *Water Microbiology, Water Supply
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15903Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21908)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21908
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39149Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16931
56546Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44924)https://www.ccug.se/strain?id=44924
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76236Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID361673.1StrainInfo: A central database for resolving microbial strain identifiers
123904Curators of the CIPCollection of Institut Pasteur (CIP 104965)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104965