Strain identifier
BacDive ID: 6764
Type strain:
Species: Legionella worsleiensis
Strain Designation: 1347, 95/83-1347
Strain history: CIP <- 1997, NCTC <- 1993, T. Harrison, LMR Colindale <- P.J. Dennis: strain 95/83-1347
NCBI tax ID(s): 45076 (species)
General
@ref: 15902
BacDive-ID: 6764
DSM-Number: 21907
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative
description: Legionella worsleiensis 1347 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from cooling tower return flow.
NCBI tax id
- NCBI tax id: 45076
- Matching level: species
strain history
@ref | history |
---|---|
15902 | <- CCUG <- CIP <- NCTC <- T. Harrison, Lab. Microbiol. Reagents (LMR), Central Public Health Lab., Colindale, London, UK <- P. J. Dennis, PHLS Salisbury, UK; 95/83-1347 |
123961 | CIP <- 1997, NCTC <- 1993, T. Harrison, LMR Colindale <- P.J. Dennis: strain 95/83-1347 |
doi: 10.13145/bacdive6764.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella worsleiensis
- full scientific name: Legionella worsleiensis Dennis et al. 1993
@ref: 15902
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella worsleiensis
full scientific name: Legionella worsleiensis Dennis et al. 1993
strain designation: 1347, 95/83-1347
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.99 | ||
123961 | negative | rod-shaped | no |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15902 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
35175 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
123961 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15902 | positive | growth | 37 | mesophilic |
35175 | positive | growth | 37 | mesophilic |
56545 | positive | growth | 37 | mesophilic |
123961 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
15902 | microaerophile |
56545 | microaerophile |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.995 |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
123961 | oxidase | + | |
123961 | catalase | - | 1.11.1.6 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123961 | - | + | + | + | - | + | + | - | - | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123961 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15902 | cooling tower return flow | Worsley | United Kingdom | GBR | Europe |
56545 | Cooling tower return flow | Worsley | United Kingdom | GBR | Europe |
123961 | Cooling tower return flow | Worsley | United Kingdom | GBR | Europe |
isolation source categories
- Cat1: #Engineered
- Cat2: #Industrial
- Cat3: #Cooling tower
taxonmaps
- @ref: 69479
- File name: preview.99_5612.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_527;97_1875;98_2302;99_5612&stattab=map
- Last taxonomy: Legionella worsleiensis
- 16S sequence: Z49739
- Sequence Identity:
- Total samples: 30
- soil counts: 3
- aquatic counts: 24
- animal counts: 3
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15902 | 1 | Risk group (German classification) |
123961 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15902
- description: L.worsliensis gene for ribosomal RNA, small subunit
- accession: Z49739
- length: 1415
- database: ena
- NCBI tax ID: 45076
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Legionella worsleiensis NCTC12377 | GCA_900453045 | contig | ncbi | 45076 |
66792 | Legionella worsleiensis ATCC 49508 | GCA_001467535 | scaffold | ncbi | 45076 |
66792 | Legionella worsleiensis strain ATCC 49508 | 45076.6 | wgs | patric | 45076 |
66792 | Legionella worsleiensis strain NCTC12377 | 45076.7 | wgs | patric | 45076 |
66792 | Legionella worsleiensis NCTC 12377 | 2808606675 | draft | img | 45076 |
66792 | Legionella worsleiensis ATCC 49508 | 2660238685 | draft | img | 45076 |
GC content
- @ref: 15902
- GC-content: 41
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 77.176 | no |
gram-positive | no | 98.173 | no |
anaerobic | no | 97.551 | yes |
halophile | no | 88.142 | no |
spore-forming | no | 93.154 | no |
glucose-util | yes | 72.924 | no |
motile | yes | 77.949 | no |
aerobic | no | 93.163 | yes |
thermophile | no | 98.489 | yes |
glucose-ferment | no | 84.198 | no |
External links
@ref: 15902
culture collection no.: DSM 21907, ATCC 49508, CCUG 44923, CIP 105114, NCTC 12377
straininfo link
- @ref: 76234
- straininfo: 361674
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15902 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21907) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21907 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
35175 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17098 | ||||
56545 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 44923) | https://www.ccug.se/strain?id=44923 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
76234 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID361674.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123961 | Curators of the CIP | Collection of Institut Pasteur (CIP 105114) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105114 |