Strain identifier

BacDive ID: 6764

Type strain: Yes

Species: Legionella worsleiensis

Strain Designation: 1347, 95/83-1347

Strain history: CIP <- 1997, NCTC <- 1993, T. Harrison, LMR Colindale <- P.J. Dennis: strain 95/83-1347

NCBI tax ID(s): 45076 (species)

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General

@ref: 15902

BacDive-ID: 6764

DSM-Number: 21907

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella worsleiensis 1347 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from cooling tower return flow.

NCBI tax id

  • NCBI tax id: 45076
  • Matching level: species

strain history

@refhistory
15902<- CCUG <- CIP <- NCTC <- T. Harrison, Lab. Microbiol. Reagents (LMR), Central Public Health Lab., Colindale, London, UK <- P. J. Dennis, PHLS Salisbury, UK; 95/83-1347
123961CIP <- 1997, NCTC <- 1993, T. Harrison, LMR Colindale <- P.J. Dennis: strain 95/83-1347

doi: 10.13145/bacdive6764.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella worsleiensis
  • full scientific name: Legionella worsleiensis Dennis et al. 1993

@ref: 15902

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella worsleiensis

full scientific name: Legionella worsleiensis Dennis et al. 1993

strain designation: 1347, 95/83-1347

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.99
123961negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15902BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
35175MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123961CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15902positivegrowth37mesophilic
35175positivegrowth37mesophilic
56545positivegrowth37mesophilic
123961positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15902microaerophile
56545microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.995

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123961oxidase+
123961catalase-1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123961-+++-++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123961---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15902cooling tower return flowWorsleyUnited KingdomGBREurope
56545Cooling tower return flowWorsleyUnited KingdomGBREurope
123961Cooling tower return flowWorsleyUnited KingdomGBREurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial
  • Cat3: #Cooling tower

taxonmaps

  • @ref: 69479
  • File name: preview.99_5612.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_527;97_1875;98_2302;99_5612&stattab=map
  • Last taxonomy: Legionella worsleiensis
  • 16S sequence: Z49739
  • Sequence Identity:
  • Total samples: 30
  • soil counts: 3
  • aquatic counts: 24
  • animal counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
159021Risk group (German classification)
1239612Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15902
  • description: L.worsliensis gene for ribosomal RNA, small subunit
  • accession: Z49739
  • length: 1415
  • database: ena
  • NCBI tax ID: 45076

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella worsleiensis NCTC12377GCA_900453045contigncbi45076
66792Legionella worsleiensis ATCC 49508GCA_001467535scaffoldncbi45076
66792Legionella worsleiensis strain ATCC 4950845076.6wgspatric45076
66792Legionella worsleiensis strain NCTC1237745076.7wgspatric45076
66792Legionella worsleiensis NCTC 123772808606675draftimg45076
66792Legionella worsleiensis ATCC 495082660238685draftimg45076

GC content

  • @ref: 15902
  • GC-content: 41

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno77.176no
gram-positiveno98.173no
anaerobicno97.551yes
halophileno88.142no
spore-formingno93.154no
glucose-utilyes72.924no
motileyes77.949no
aerobicno93.163yes
thermophileno98.489yes
glucose-fermentno84.198no

External links

@ref: 15902

culture collection no.: DSM 21907, ATCC 49508, CCUG 44923, CIP 105114, NCTC 12377

straininfo link

  • @ref: 76234
  • straininfo: 361674

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15902Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21907)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21907
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35175Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17098
56545Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44923)https://www.ccug.se/strain?id=44923
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76234Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID361674.1StrainInfo: A central database for resolving microbial strain identifiers
123961Curators of the CIPCollection of Institut Pasteur (CIP 105114)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105114