Strain identifier
BacDive ID: 6762
Type strain:
Species: Legionella nautarum
Strain Designation: 1224, Thacker 1224
Strain history: CIP <- 1997, CDC <- W.L. Thacker: strain 1224 <- P.J. Dennis
NCBI tax ID(s): 45070 (species)
General
@ref: 15966
BacDive-ID: 6762
DSM-Number: 21805
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative
description: Legionella nautarum 1224 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Hot water tap.
NCBI tax id
- NCBI tax id: 45070
- Matching level: species
strain history
@ref | history |
---|---|
15966 | <- CCUG <- CIP {2001} <- CDC {1997} <- W. L. Thacker; 1224 <- P. J. Dennis |
121245 | CIP <- 1997, CDC <- W.L. Thacker: strain 1224 <- P.J. Dennis |
doi: 10.13145/bacdive6762.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella nautarum
- full scientific name: Legionella nautarum Dennis et al. 1993
@ref: 15966
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella nautarum
full scientific name: Legionella nautarum Dennis et al. 1993
strain designation: 1224, Thacker 1224
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.998 | ||
121245 | negative | rod-shaped | no |
colony morphology
- @ref: 15966
- incubation period: 8-14 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15966 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
40139 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
121245 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15966 | positive | growth | 37 | mesophilic |
40139 | positive | growth | 37 | mesophilic |
56537 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
15966 | microaerophile |
56537 | microaerophile |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.972 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121245 | 17632 | nitrate | - | reduction |
121245 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 121245
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
121245 | oxidase | + | |
121245 | catalase | + | 1.11.1.6 |
121245 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121245 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15966 | Hot water tap | London | United Kingdom | GBR | Europe |
56537 | Hot water tap | England,London | United Kingdom | GBR | Europe |
121245 | Environment, Hot water tap | London | United Kingdom | GBR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_85194.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_3174;97_3892;98_59009;99_85194&stattab=map
- Last taxonomy: Legionella
- 16S sequence: Z49730
- Sequence Identity:
- Total samples: 8
- soil counts: 6
- animal counts: 1
- plant counts: 1
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15966 | 1 | Risk group (German classification) |
121245 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15966
- description: Legionella londiniensis gene for ribosomal RNA, small subunit
- accession: Z49730
- length: 1411
- database: ena
- NCBI tax ID: 45068
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Legionella nautarum ATCC 49506 | GCA_001467895 | scaffold | ncbi | 45070 |
66792 | Legionella nautarum 1224 | GCA_900639935 | scaffold | ncbi | 45070 |
66792 | Legionella nautarum strain ATCC 49506 | 45070.6 | wgs | patric | 45070 |
66792 | Legionella nautarum ATCC 49506 | 2681813188 | draft | img | 45070 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 76.208 | no |
gram-positive | no | 98.629 | no |
anaerobic | no | 96.908 | yes |
halophile | no | 84.925 | no |
spore-forming | no | 92.82 | no |
glucose-util | yes | 57.882 | no |
motile | yes | 80.616 | no |
aerobic | no | 88.412 | yes |
thermophile | no | 98.422 | yes |
glucose-ferment | no | 89.067 | no |
External links
@ref: 15966
culture collection no.: DSM 21805, ATCC 49506, CCUG 44900, CIP 105270, NCTC 12375
straininfo link
- @ref: 76232
- straininfo: 42522
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
15966 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21805) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21805 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
40139 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17271 | ||
56537 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 44900) | https://www.ccug.se/strain?id=44900 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
76232 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42522.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
121245 | Curators of the CIP | Collection of Institut Pasteur (CIP 105270) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105270 |