Strain identifier

BacDive ID: 6762

Type strain: Yes

Species: Legionella nautarum

Strain Designation: 1224, Thacker 1224

Strain history: CIP <- 1997, CDC <- W.L. Thacker: strain 1224 <- P.J. Dennis

NCBI tax ID(s): 45070 (species)

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General

@ref: 15966

BacDive-ID: 6762

DSM-Number: 21805

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella nautarum 1224 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Hot water tap.

NCBI tax id

  • NCBI tax id: 45070
  • Matching level: species

strain history

@refhistory
15966<- CCUG <- CIP {2001} <- CDC {1997} <- W. L. Thacker; 1224 <- P. J. Dennis
121245CIP <- 1997, CDC <- W.L. Thacker: strain 1224 <- P.J. Dennis

doi: 10.13145/bacdive6762.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella nautarum
  • full scientific name: Legionella nautarum Dennis et al. 1993

@ref: 15966

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella nautarum

full scientific name: Legionella nautarum Dennis et al. 1993

strain designation: 1224, Thacker 1224

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.998
121245negativerod-shapedno

colony morphology

  • @ref: 15966
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15966BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40139MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121245CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15966positivegrowth37mesophilic
40139positivegrowth37mesophilic
56537positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15966microaerophile
56537microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.972

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12124517632nitrate-reduction
12124516301nitrite-reduction

metabolite production

  • @ref: 121245
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121245oxidase+
121245catalase+1.11.1.6
121245urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121245-+++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15966Hot water tapLondonUnited KingdomGBREurope
56537Hot water tapEngland,LondonUnited KingdomGBREurope
121245Environment, Hot water tapLondonUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_85194.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_3174;97_3892;98_59009;99_85194&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: Z49730
  • Sequence Identity:
  • Total samples: 8
  • soil counts: 6
  • animal counts: 1
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
159661Risk group (German classification)
1212452Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15966
  • description: Legionella londiniensis gene for ribosomal RNA, small subunit
  • accession: Z49730
  • length: 1411
  • database: ena
  • NCBI tax ID: 45068

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella nautarum ATCC 49506GCA_001467895scaffoldncbi45070
66792Legionella nautarum 1224GCA_900639935scaffoldncbi45070
66792Legionella nautarum strain ATCC 4950645070.6wgspatric45070
66792Legionella nautarum ATCC 495062681813188draftimg45070

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno76.208no
gram-positiveno98.629no
anaerobicno96.908yes
halophileno84.925no
spore-formingno92.82no
glucose-utilyes57.882no
motileyes80.616no
aerobicno88.412yes
thermophileno98.422yes
glucose-fermentno89.067no

External links

@ref: 15966

culture collection no.: DSM 21805, ATCC 49506, CCUG 44900, CIP 105270, NCTC 12375

straininfo link

  • @ref: 76232
  • straininfo: 42522

Reference

@idauthorscataloguedoi/urltitle
15966Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21805)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21805
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40139Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17271
56537Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44900)https://www.ccug.se/strain?id=44900
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76232Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42522.1StrainInfo: A central database for resolving microbial strain identifiers
121245Curators of the CIPCollection of Institut Pasteur (CIP 105270)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105270