Strain identifier

BacDive ID: 6761

Type strain: Yes

Species: Legionella geestiana

Strain Designation: 1308

Strain history: CIP <- 1998, CDC <- P.J. Dennis: strain 1308

NCBI tax ID(s): 1122161 (strain), 45065 (species)

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General

@ref: 15583

BacDive-ID: 6761

DSM-Number: 21217

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella geestiana 1308 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from domestic hot water.

NCBI tax id

NCBI tax idMatching level
45065species
1122161strain

strain history

@refhistory
15583<- CIP; strain 1308 {2007} <- CDC <- P. J. Dennis
119560CIP <- 1998, CDC <- P.J. Dennis: strain 1308

doi: 10.13145/bacdive6761.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella geestiana
  • full scientific name: Legionella geestiana Dennis et al. 1993

@ref: 15583

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella geestiana

full scientific name: Legionella geestiana Dennis et al. 1993

strain designation: 1308

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.995
119560negativerod-shapedyes

colony morphology

  • @ref: 15583
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15583BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34051MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119560CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15583positivegrowth37mesophilic
34051positivegrowth37mesophilic
56531positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56531microaerophile
119560obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11956017632nitrate-reduction
11956016301nitrite-reduction

metabolite production

  • @ref: 119560
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
15583catalase-1.11.1.6
15583cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119560oxidase-
119560catalase+1.11.1.6
119560urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    56531C14:02.614
    56531C15:01.715
    56531C16:013.116
    56531C20:00.720
    56531C13:0 iso0.912.612
    56531C14:1 ω5c2.813.899
    56531C15:0 ANTEISO814.711
    56531C15:0 ISO13.414.621
    56531C15:0 ISO 3OH0.416.135
    56531C15:1 ω6c2.614.856
    56531C16:0 iso0.515.626
    56531C16:1 ω7c47.515.819
    56531C17:0 anteiso1.816.722
    56531C17:0 iso2.916.629
    56531C17:1 ω9c ISO0.816.416
    56531Unidentified0.417.953
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119560-+++++++--++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
15583domestic hot waterUnited KingdomGBREurope
56531Domestic hot waterUnited KingdomGBREuropeLondon
119560Environment, Domestic hot waterUnited KingdomGBREuropeLondon

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_7473.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_3489;97_4277;98_5475;99_7473&stattab=map
  • Last taxonomy: Legionella geestiana subclade
  • 16S sequence: Z49723
  • Sequence Identity:
  • Total samples: 1955
  • soil counts: 391
  • aquatic counts: 1241
  • animal counts: 259
  • plant counts: 64

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
155831Risk group (German classification)
1195602Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15583
  • description: L.geestiana gene for ribosomal RNA, small subunit
  • accession: Z49723
  • length: 1417
  • database: ena
  • NCBI tax ID: 45065

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella geestiana 1308GCA_004571195completencbi45065
66792Legionella geestiana NCTC12373GCA_900452375contigncbi45065
66792Legionella geestiana 1308GCA_900639805contigncbi45065
66792Legionella geestiana DSM 21217GCA_000621365scaffoldncbi1122161
66792Legionella geestiana ATCC 49504GCA_001467645scaffoldncbi45065
66792Legionella geestiana DSM 212171122161.4wgspatric1122161
66792Legionella geestiana strain 130845065.6completepatric45065
66792Legionella geestiana strain ATCC 4950445065.4wgspatric45065
66792Legionella geestiana strain NCTC1237345065.5wgspatric45065
66792Legionella geestiana NCTC 123732808606774draftimg45065
66792Legionella geestiana DSM 212172556921651draftimg1122161
66792Legionella geestiana 13082859579839completeimg45065
66792Legionella geestiana ATCC 495042693429649draftimg45065

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno79.12no
gram-positiveno98.535no
anaerobicno97.499yes
aerobicno93.147yes
halophileno87.974no
spore-formingno94.721no
glucose-utilyes62.118no
thermophileno97.68yes
motileyes78.026no
glucose-fermentno89.511no

External links

@ref: 15583

culture collection no.: DSM 21217, ATCC 49504, CCUG 44893, CIP 105569, NCTC 12373

straininfo link

  • @ref: 76231
  • straininfo: 42521

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15583Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21217)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21217
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34051Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17603
56531Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44893)https://www.ccug.se/strain?id=44893
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76231Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42521.1StrainInfo: A central database for resolving microbial strain identifiers
119560Curators of the CIPCollection of Institut Pasteur (CIP 105569)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105569