Strain identifier

BacDive ID: 6759

Type strain: Yes

Species: Legionella gratiana

Strain Designation: 8420412

Strain history: CIP <- 1997, CDC <- N. Bornstein, Lyon, France: strain 8420412

NCBI tax ID(s): 45066 (species)

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General

@ref: 15586

BacDive-ID: 6759

DSM-Number: 21233

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Legionella gratiana 8420412 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from thermal spa.

NCBI tax id

  • NCBI tax id: 45066
  • Matching level: species

strain history

@refhistory
15586<- CIP; Lyon 8420412 {2008} <- CDC <- N. Bornstein
119732CIP <- 1997, CDC <- N. Bornstein, Lyon, France: strain 8420412

doi: 10.13145/bacdive6759.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella gratiana
  • full scientific name: Legionella gratiana Bornstein et al. 1991

@ref: 15586

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella gratiana

full scientific name: Legionella gratiana Bornstein et al. 1991

strain designation: 8420412

type strain: yes

Morphology

cell morphology

  • @ref: 119732
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 15586
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15586BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
39243MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119732CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15586positivegrowth37mesophilic
39243positivegrowth37mesophilic
56532positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15586microaerophile
56532microaerophile

enzymes

@refvalueactivityec
15586catalase+1.11.1.6
15586cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1

fatty acid profile

  • @reffatty acidpercentageECL
    56532C14:00.914
    56532C15:02.615
    56532C16:06.716
    56532C17:00.817
    56532C14:0 ISO11.413.618
    56532C14:1 ω5c1.413.899
    56532C15:0 ANTEISO12.714.711
    56532C15:0 ISO0.514.621
    56532C15:1 ω6c4.814.856
    56532C16:0 iso23.615.626
    56532C16:1 ω5c1.415.908
    56532C16:1 ω7c24.915.819
    56532C17:0 anteiso1.716.722
    56532C17:0 CYCLO6.516.888
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    56532C14:00.614
    56532C15:01.215
    56532C16:04.816
    56532C17:00.617
    56532C14:0 ISO12.113.618
    56532C14:1 ω5c0.913.899
    56532C15:0 ANTEISO11.914.711
    56532C15:0 ISO0.614.621
    56532C15:1 ω6c4.414.856
    56532C16:0 iso25.115.626
    56532C16:0 iso 3OH0.717.145
    56532C16:1 ISO H0.515.461
    56532C16:1 ω5c115.908
    56532C16:1 ω7c26.215.819
    56532C17:0 anteiso1.416.722
    56532C17:0 CYCLO7.916.888
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119732-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
15586thermal spaFranceFRAEurope
56532Thermal spa waterFranceFRAEuropeSavoie
119732Environment, Thermal spa waterFranceFRAEuropeSavoie

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
15586yes, in single cases1Risk group (German classification)
1197322Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15586
  • description: L.gratiana gene for ribosomal RNA, small subunit
  • accession: Z49725
  • length: 1400
  • database: ena
  • NCBI tax ID: 45066

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella gratiana NCTC12388GCA_900452545contigncbi45066
66792Legionella gratiana strain Lyon 842041245066.5wgspatric45066
66792Legionella gratiana strain NCTC1238845066.7wgspatric45066
66792Legionella gratiana NCTC 123882925233983draftimg45066

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.359no
anaerobicno98.489no
halophileno90.509no
spore-formingno92.986no
glucose-utilyes71.293no
aerobicno90.852yes
flagellatedno95.478no
motileno72.942no
thermophileno98.95yes
glucose-fermentno88.976no

External links

@ref: 15586

culture collection no.: DSM 21233, ATCC 49413, CCUG 44894, CIP 105267, NCTC 12388, Lyon 8420412

straininfo link

  • @ref: 76229
  • straininfo: 93040

literature

  • topic: Phylogeny
  • Pubmed-ID: 2696060
  • title: Legionella gratiana sp. nov. isolated from French spa water.
  • authors: Bornstein N, Marmet D, Surgot M, Nowicki M, Meugnier H, Fleurette J, Ageron E, Grimont F, Grimont PA, Thacker WL, et al.
  • journal: Res Microbiol
  • DOI: 10.1016/0923-2508(89)90086-7
  • year: 1989
  • mesh: Antibodies/analysis, Bacterial Proteins/analysis, DNA, Bacterial/analysis, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Fluorescent Antibody Technique, Humans, Legionella/immunology/*isolation & purification/metabolism, Mineral Waters/*analysis, Ubiquinone/analysis
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitle
15586Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21233)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21233
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39243Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17268
56532Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44894)https://www.ccug.se/strain?id=44894
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
76229Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID93040.1StrainInfo: A central database for resolving microbial strain identifiers
119732Curators of the CIPCollection of Institut Pasteur (CIP 105267)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105267