Strain identifier

BacDive ID: 6748

Type strain: Yes

Species: Legionella hackeliae

Strain history: CIP <- 1993, R.F. Benson, CDC: strain Lansing 2 <- B.B. Wentworth <- M. Hackel

NCBI tax ID(s): 449 (species)

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General

@ref: 7972

BacDive-ID: 6748

DSM-Number: 19214

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, motile

description: Legionella hackeliae DSM 19214 is a microaerophile, mesophilic, motile bacterium that was isolated from human bronchial biopsy.

NCBI tax id

  • NCBI tax id: 449
  • Matching level: species

strain history

@refhistory
7972<- H. E. Müller <- ATCC <- W. F. Bibb <- B. B. Wentworth <- M. Hackel
67770GIFU 10740 <-- CDC, strain Lansing 2 <-- B. B. Wentworth <-- M. Hackel.
119759CIP <- 1993, R.F. Benson, CDC: strain Lansing 2 <- B.B. Wentworth <- M. Hackel

doi: 10.13145/bacdive6748.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella hackeliae
  • full scientific name: Legionella hackeliae Brenner et al. 1985

@ref: 7972

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella hackeliae

full scientific name: Legionella hackeliae Brenner et al. 1985

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.543
6948099.999negative
119759nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7972BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40810MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119759CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7972positivegrowth37mesophilic
40810positivegrowth37mesophilic
50487positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7972microaerophile
50487microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11975917632nitrate-reduction
11975916301nitrite-reduction

metabolite production

  • @ref: 119759
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119759oxidase-
119759catalase+1.11.1.6
119759urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119759-+---+++--++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7972human bronchial biopsyAnn ArborUSAUSANorth America
50487Human bronchial biopsyMichigan,Ann ArborUSAUSANorth America
67770Bronchial biopsy, human pneumoniaAnn Arbor, MIUSAUSANorth America
119759Human, Bronchial biopsyAnn Arbor, MichiganUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Biopsy
#Host#Human
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_1835.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_1036;97_1210;98_1450;99_1835&stattab=map
  • Last taxonomy: Legionella hackeliae subclade
  • 16S sequence: LN681225
  • Sequence Identity:
  • Total samples: 131
  • soil counts: 13
  • aquatic counts: 102
  • animal counts: 14
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79722Risk group (German classification)
1197592Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella hackeliae strain MDC1585 16S ribosomal RNA gene, partial sequenceJF720375525ena449
7972Legionella hackeliae genome assembly LHA, chromosome : ILN6812253440975ena449
67770Legionella hackeliae partial 16S rRNA gene, strain Lancing 2HF5583771518ena449
67770Legionella hackeliae JCM 7563 gene for 16S ribosomal RNA, partial sequenceLC5040401467ena449
67770Legionella hackeliae 16S rRNA sequenceM360281473ena449

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella hackeliae ATCC 35250GCA_000953655completencbi449
66792Legionella hackeliae strain ATCC35250449.5completepatric449
66792Legionella hackeliae strain NCTC11979449.8wgspatric449
66792Legionella hackeliae ATCC352502654588001completeimg449
67770Legionella hackeliae NCTC11979GCA_900452565contigncbi449

GC content

  • @ref: 67770
  • GC-content: 40
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes81.09no
flagellatedno79.681no
gram-positiveno98.051no
anaerobicno98.389yes
aerobicno87.129yes
halophileno90.364no
spore-formingno93.028no
glucose-utilyes72.066no
thermophileno99.226yes
glucose-fermentno85.275no

External links

@ref: 7972

culture collection no.: DSM 19214, ATCC 35250, CIP 103844, JCM 7563, Lansing 2, NCTC 11979, CCUG 31232 A

straininfo link

  • @ref: 76218
  • straininfo: 125847

literature

  • topic: Phylogeny
  • Pubmed-ID: 4077960
  • title: Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.
  • authors: Wilkinson HW, Thacker WL, Steigerwalt AG, Brenner DJ, Ampel NM, Wing EJ
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.22.4.488-489.1985
  • year: 1985
  • mesh: Agglutination Tests, DNA, Bacterial/analysis, Female, Humans, Legionella/classification/*isolation & purification/physiology, Legionnaires' Disease/*microbiology, Middle Aged, Nucleic Acid Hybridization, Pneumonia/*microbiology, Serotyping
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7972Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19214)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19214
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40810Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15686
50487Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31232 A)https://www.ccug.se/strain?id=31232
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76218Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125847.1StrainInfo: A central database for resolving microbial strain identifiers
119759Curators of the CIPCollection of Institut Pasteur (CIP 103844)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103844