Strain identifier

BacDive ID: 6746

Type strain: Yes

Species: Legionella wadsworthii

Strain Designation: Edelstein Wadsworth 81-716A, 81-716A

Strain history: CIP <- 2000, F. Grimont, Inst. Pasteur, Paris, France <- CNR Legionella, Lyon, France <- P.H. Edelstein: strain Wadsworth 81-716A

NCBI tax ID(s): 1122170 (strain), 28088 (species)

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General

@ref: 16025

BacDive-ID: 6746

DSM-Number: 21896

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella wadsworthii Edelstein Wadsworth 81-716A is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Human sputum; patient with pneumonia.

NCBI tax id

NCBI tax idMatching level
1122170strain
28088species

strain history

@refhistory
16025<- CCUG <- CDC
123901CIP <- 2000, F. Grimont, Inst. Pasteur, Paris, France <- CNR Legionella, Lyon, France <- P.H. Edelstein: strain Wadsworth 81-716A

doi: 10.13145/bacdive6746.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella wadsworthii
  • full scientific name: Legionella wadsworthii Edelstein et al. 1983

@ref: 16025

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella wadsworthii

full scientific name: Legionella wadsworthii Edelstein et al. 1983

strain designation: Edelstein Wadsworth 81-716A, 81-716A

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.994
123901negativerod-shapedno

colony morphology

  • @ref: 16025
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16025BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34430MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
123901CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
16025positivegrowth37mesophilic
34430positivegrowth37mesophilic
123901positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 16025
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.986

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123901oxidase+
123901catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123901-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16025Human sputum; patient with pneumoniaLos Angeles, CaliforniaUSAUSANorth America
123901Human, Sputum

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host#Human
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_142115.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_94420;99_142115&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: Z49738
  • Sequence Identity:
  • Total samples: 44
  • soil counts: 10
  • aquatic counts: 33
  • animal counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
160252Risk group (German classification)
1239012Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218L.wadsworthii gene for ribosomal RNA, small subunitZ497381472ena28088
16025L.wadsworthii (ATCC 33877) gene for 16S rRNAX734011467ena28088

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella wadsworthii NCTC11532GCA_900452925contigncbi28088
66792Legionella wadsworthii DSM 21896 = ATCC 33877GCA_000701265scaffoldncbi1122170
66792Legionella wadsworthii DSM 21896 = ATCC 338771122170.4wgspatric1122170
66792Legionella wadsworthii strain NCTC1153228088.5wgspatric28088
66792Legionella wadsworthii ATCC 338772561511070draftimg1122170

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno72.536no
flagellatedno94.55no
gram-positiveno98.537no
anaerobicno98.037no
halophileno86.84no
spore-formingno94.545no
glucose-utilyes73.557no
aerobicno90.909yes
thermophileno99.039yes
glucose-fermentno85.454no

External links

@ref: 16025

culture collection no.: DSM 21896, ATCC 33877, CCUG 16415, CIP 103886, NCTC 11532, Wadsworth 81-716, CCUG 58308, Wadsworth 81-716A

straininfo link

  • @ref: 76216
  • straininfo: 40739

literature

  • topic: Pathogenicity
  • Pubmed-ID: 6756238
  • title: Legionella wadsworthii species nova: a cause of human pneumonia.
  • authors: Edelstein PH, Brenner DJ, Moss CW, Steigerwalt AG, Francis EM, George WL
  • journal: Ann Intern Med
  • DOI: 10.7326/0003-4819-97-6-809
  • year: 1982
  • mesh: Aged, Bacteriological Techniques, Erythromycin/therapeutic use, Humans, Legionella/*classification, Legionnaires' Disease/drug therapy/*microbiology, Leukemia, Lymphoid/complications, Male, Pneumonia/drug therapy/*microbiology, Rifampin/therapeutic use, Serologic Tests
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16025Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21896)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21896
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34430Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15732
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76216Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40739.1StrainInfo: A central database for resolving microbial strain identifiers
123901Curators of the CIPCollection of Institut Pasteur (CIP 103886)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103886