Strain identifier

BacDive ID: 6744

Type strain: Yes

Species: Legionella sainthelensi

Strain Designation: MSH-4, Mt. St. Helens 4, strain 4

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.F. Bibb: strain MSH-4 <- J. Campbell: strain 4

NCBI tax ID(s): 1408445 (strain), 28087 (species)

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General

@ref: 7980

BacDive-ID: 6744

DSM-Number: 19231

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella sainthelensi MSH-4 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from spring water.

NCBI tax id

NCBI tax idMatching level
28087species
1408445strain

strain history

@refhistory
7980<- H. E. Müller <- ATCC <- W. F. Bibb; MSH-4 <- J. Campbell; strain 4
122351CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.F. Bibb: strain MSH-4 <- J. Campbell: strain 4

doi: 10.13145/bacdive6744.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella sainthelensi
  • full scientific name: Legionella sainthelensi Campbell et al. 1984

@ref: 7980

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella sainthelensi

full scientific name: Legionella sainthelensi Campbell et al. 1984

strain designation: MSH-4, Mt. St. Helens 4, strain 4

type strain: yes

Morphology

cell morphology

  • @ref: 122351
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 7980
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7980BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34085MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122351CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7980positivegrowth37mesophilic
34085positivegrowth37mesophilic
49708positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7980microaerophile
49708microaerophile

enzymes

@refvalueactivityec
7980catalase+1.11.1.6
7980cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122351--------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7980spring waterMt. St. HelensUSAUSANorth America
49708Stream water1981
122351Environment, Spring waterMount Saint Helens, WashingtonUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_5784.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_671;99_5784&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: Z49734
  • Sequence Identity:
  • Total samples: 1414
  • soil counts: 142
  • aquatic counts: 1090
  • animal counts: 151
  • plant counts: 31

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79802Risk group (German classification)
1223512Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella sainthelensi strain MDC1588 16S ribosomal RNA gene, partial sequenceJF720399524ena28087
20218L.sainthelensi gene for ribosomal RNA, small subunitZ497341405ena28087
7980L.sainthelensi (ATCC 35248) gene for 16S rRNAX733991467ena28087

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella sainthelensi NCTC11988GCA_900637685completencbi28087
66792Legionella sainthelensi MSH-4GCA_900640185contigncbi28087
66792Legionella sainthelensi ATCC 35248 = DSM 19231GCA_000621685scaffoldncbi1408445
66792Legionella sainthelensi ATCC 352481408445.3wgspatric1408445
66792Legionella sainthelensi strain Mt.St.Helens-428087.4wgspatric28087
66792Legionella sainthelensi strain NCTC1198828087.10completepatric28087
66792Legionella sainthelensi NCTC119882848179527completeimg28087
66792Legionella sainthelensi ATCC 352482558309002draftimg1408445

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.969no
gram-positiveno98.233no
anaerobicno99.176no
aerobicno81.544no
halophileno87.448no
spore-formingno93.015no
thermophileno99.402yes
glucose-utilyes68.321no
motileno71.204no
glucose-fermentno89.143no

External links

@ref: 7980

culture collection no.: DSM 19231, ATCC 35248, CCUG 29672, CIP 103885, NCTC 11988

straininfo link

  • @ref: 76214
  • straininfo: 40945

literature

  • topic: Phylogeny
  • Pubmed-ID: 6712210
  • title: Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.
  • authors: Campbell J, Bibb WF, Lambert MA, Eng S, Steigerwalt AG, Allard J, Moss CW, Brenner DJ
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/aem.47.2.369-373.1984
  • year: 1984
  • mesh: Disasters, *Fresh Water, Legionella/classification/*isolation & purification/metabolism, Washington, *Water, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7980Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19231)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19231
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34085Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15731
49708Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29672)https://www.ccug.se/strain?id=29672
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76214Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40945.1StrainInfo: A central database for resolving microbial strain identifiers
122351Curators of the CIPCollection of Institut Pasteur (CIP 103885)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103885