Strain identifier

BacDive ID: 6740

Type strain: Yes

Species: Legionella cincinnatiensis

Strain Designation: 72-OH-H

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 72-OH-H <- J.L. Staneck

NCBI tax ID(s): 28085 (species)

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General

@ref: 7982

BacDive-ID: 6740

DSM-Number: 19233

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Legionella cincinnatiensis 72-OH-H is a mesophilic, Gram-negative bacterium that was isolated from human lung tissue.

NCBI tax id

  • NCBI tax id: 28085
  • Matching level: species

strain history

@refhistory
7982<- H. E. Müller <- ATCC <- W. L. Thacker; 72-OH-H <- J. L. Stanek
118283CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 72-OH-H <- J.L. Staneck

doi: 10.13145/bacdive6740.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella cincinnatiensis
  • full scientific name: Legionella cincinnatiensis Thacker et al. 1989

@ref: 7982

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella cincinnatiensis

full scientific name: Legionella cincinnatiensis Thacker et al. 1989

strain designation: 72-OH-H

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.998
118283negativerod-shapedyes

colony morphology

  • @ref: 7982
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7982BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40843MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
118283CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7982positivegrowth37mesophilic
40843positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.981

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

enzymes

@refvalueactivityec
7982catalase+1.11.1.6
7982cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
118283oxidase-
118283catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118283-+++-+++--++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118283-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118283---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7982human lung tissueCincinnatiUSAUSANorth America
50485Human lung tissueOhio,CincinnatiUSAUSANorth America
118283Human, Lung tissueCincinnati, OhioUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_6709.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_671;99_6709&stattab=map
  • Last taxonomy: Legionella cincinnatiensis
  • 16S sequence: Z49721
  • Sequence Identity:
  • Total samples: 10
  • soil counts: 4
  • aquatic counts: 2
  • animal counts: 2
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79822Risk group (German classification)
1182832Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella cincinnatiensis strain MDC1590 16S ribosomal RNA gene, partial sequenceJF720400525ena28085
20218L.cincinatiensis (ATCC 43753) gene for 16S rRNAX734071468ena28085
7982L.cicinatiensis gene for ribosomal RNA, small subunitZ497211416ena28085

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella cincinnatiensis NCTC12438GCA_900452415contigncbi28085
66792Legionella cincinnatiensis strain CDC#72-OH-1428085.5wgspatric28085
66792Legionella cincinnatiensis strain NCTC1243828085.6wgspatric28085
66792Legionella cincinnatiensis NCTC 124382907323874draftimg28085
66792Legionella cincinnatiensis ATCC437532693429757draftimg28085

GC content

  • @ref: 7982
  • GC-content: 36.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno71.434no
gram-positiveno97.956no
anaerobicno98.791no
halophileno86.256no
spore-formingno93.171no
glucose-utilyes67.104no
flagellatedno94.49no
aerobicno84.818no
thermophileno99.45yes
glucose-fermentno86.928no

External links

@ref: 7982

culture collection no.: DSM 19233, ATCC 43753, CCUG 31230 A, CIP 103875, NCTC 12438

straininfo link

  • @ref: 76210
  • straininfo: 42022

literature

  • topic: Phylogeny
  • Pubmed-ID: 3281971
  • title: Legionella cincinnatiensis sp. nov. isolated from a patient with pneumonia.
  • authors: Thacker WL, Benson RF, Staneck JL, Vincent SR, Mayberry WR, Brenner DJ, Wilkinson HW
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.26.3.418-420.1988
  • year: 1988
  • mesh: Adult, Agglutination Tests, Biopsy, Bronchopneumonia/*microbiology, Chromatography, Gas, DNA, Bacterial/genetics, Fluorescent Antibody Technique, Humans, Legionella/classification/genetics/*isolation & purification, Legionellosis/*microbiology, Lung/microbiology, Male, Nucleic Acid Hybridization
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7982Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19233)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19233
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40843Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15720
50485Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31230 A)https://www.ccug.se/strain?id=31230
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76210Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42022.1StrainInfo: A central database for resolving microbial strain identifiers
118283Curators of the CIPCollection of Institut Pasteur (CIP 103875)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103875