Strain identifier

BacDive ID: 6739

Type strain: Yes

Species: Legionella brunensis

Strain Designation: 441-1

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- H.W. Wilkinson: strain 441-1 <- V. Drasar

NCBI tax ID(s): 29422 (species)

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General

@ref: 7903

BacDive-ID: 6739

DSM-Number: 19236

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella brunensis 441-1 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water cooling tower.

NCBI tax id

  • NCBI tax id: 29422
  • Matching level: species

strain history

@refhistory
7903<- H. E. Müller <- ATCC <- H. W. Wilkinson; 441-1 <- V. Drasar
117062CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- H.W. Wilkinson: strain 441-1 <- V. Drasar

doi: 10.13145/bacdive6739.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella brunensis
  • full scientific name: Legionella brunensis Wilkinson et al. 1989

@ref: 7903

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella brunensis

full scientific name: Legionella brunensis Wilkinson et al. 1989

strain designation: 441-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
117062negativerod-shapedno
125439negative99.7
125438negative98.494

colony morphology

  • @ref: 7903
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7903BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40842MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
117062CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperature
7903positivegrowth37
40842positivegrowth37
50430positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
7903microaerophile
50430microaerophile
125439obligate aerobe99

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 99.1

Isolation, sampling and environmental information

isolation

@refsample typecountrycontinentgeographic locationorigin.country
7903water cooling towerCzechoslovakiaEurope
50430Water,cooling water towerCzechoslovakiaEuropeVyskov
117062Environment, Cooling tower waterCzech RepublicEuropeCZE

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_5629.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_1036;97_3328;98_4195;99_5629&stattab=map
  • Last taxonomy: Legionella brunensis
  • 16S sequence: NR_026520
  • Sequence Identity:
  • Total samples: 63
  • soil counts: 30
  • aquatic counts: 23
  • animal counts: 9
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7903yes, in single cases1Risk group (German classification)
1170622Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella brunensis strain MDC1593 16S ribosomal RNA gene, partial sequenceJF720377525nuccore29422
20218L.brunensis (441-1) gene for 16S ribosomal RNAZ326361473nuccore29422
7903Legionella brunensis strain 441-1 16S ribosomal RNA, partial sequenceNR_0265201473nuccore29422

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella brunensis ATCC 43878GCA_001467025scaffoldncbi29422
66792Legionella brunensis 441-1GCA_900639795scaffoldncbi29422
66792Legionella brunensis strain ATCC 4387829422.6wgspatric29422
66792Legionella brunensis ATCC 438782690316045draftimg29422

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno98.494no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.251yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no76.194yes
125438spore-formingspore-formingAbility to form endo- or exosporesno88.428no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno93.294yes
125438motile2+flagellatedAbility to perform flagellated movementno54.038no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno99.1
125439BacteriaNetmotilityAbility to perform movementyes60.3
125439BacteriaNetgram_stainReaction to gram-stainingnegative99.7
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe99

External links

@ref: 7903

culture collection no.: DSM 19236, ATCC 43878, CCUG 31114, CIP 103874, NCTC 12240

straininfo link

  • @ref: 76209
  • straininfo: 42101

literature

  • topic: Phylogeny
  • Pubmed-ID: 3179063
  • title: Legionella moravica sp. nov. and Legionella brunensis sp. nov. isolated from cooling-tower water.
  • authors: Wilkinson HW, Drasar V, Thacker WL, Benson RF, Schindler J, Potuznikova B, Mayberry WR, Brenner DJ
  • journal: Ann Inst Pasteur Microbiol
  • DOI: 10.1016/0769-2609(88)90102-0
  • year: 1988
  • mesh: Czechoslovakia, Legionella/analysis/classification/*isolation & purification, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7903Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19236)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19236
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40842Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15719
50430Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31114)https://www.ccug.se/strain?id=31114
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76209Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42101.1StrainInfo: A central database for resolving microbial strain identifiers
117062Curators of the CIPCollection of Institut Pasteur (CIP 103874)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103874
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1