Strain identifier

BacDive ID: 6735

Type strain: No

Species: Legionella feeleii

Strain Designation: R29, 691-WI-H

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- L. Thacker: strain 691-WI-H <- Wisconsin State Lab. Hyg., USA

NCBI tax ID(s): 453 (species)

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General

@ref: 17995

BacDive-ID: 6735

DSM-Number: 25316

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, motile

description: Legionella feeleii R29 is a microaerophile, mesophilic, motile bacterium that was isolated from human lung tissue.

NCBI tax id

  • NCBI tax id: 453
  • Matching level: species

strain history

@refhistory
17995<- H. E. Müller; ATCC 35849 <- ATCC <- W. L. Thacker <- Wisconsin State Lab. Hyg.; 691-WI-H
119296CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- L. Thacker: strain 691-WI-H <- Wisconsin State Lab. Hyg., USA

doi: 10.13145/bacdive6735.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella feeleii
  • full scientific name: Legionella feeleii Herwaldt et al. 1984

@ref: 17995

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella feeleii

full scientific name: Legionella feeleii Herwaldt et al. 1984

strain designation: R29, 691-WI-H

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes90.688
6948099.999negative
119296nonegativerod-shaped

colony morphology

  • @ref: 17995
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17995BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
35152MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119296CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
17995positivegrowth37mesophilic
35152positivegrowth37mesophilic
49704positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17995microaerophile
49704microaerophile

spore formation

@refspore formationconfidence
69481no99
69480no99.997

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11929617632nitrate-reduction
11929616301nitrite-reduction

metabolite production

  • @ref: 119296
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
17995catalase+1.11.1.6
17995cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119296oxidase-
119296catalase+1.11.1.6
119296urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119296-+---++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17995human lung tissueWisconsinUSAUSANorth America
49704Human lung tissueWisconsinUSAUSANorth America
119296Human, Lung tissueWisconsinUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung
#Infection#Patient

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179952Risk group (German classification)
1192962Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: L.feeleii sgp2 (ATCC 35849) gene for 16S rRNA
  • accession: X73406
  • length: 1466
  • database: ena
  • NCBI tax ID: 453

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella feeleii NCTC11978GCA_900461565contigncbi453
66792Legionella feeleii strain NCTC11978453.7wgspatric453

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
gram-positiveno98.665no
anaerobicno97.393yes
halophileno87.55no
spore-formingno92.217no
glucose-utilyes59.531no
aerobicno93.808yes
flagellatedno64.255no
thermophileno96.194yes
motileyes88.071no
glucose-fermentno85.973no

External links

@ref: 17995

culture collection no.: DSM 25316, ATCC 35849, CCUG 29668, CIP 103878, NCTC 11978

straininfo link

  • @ref: 76205
  • straininfo: 46315

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny3894411Second serogroup of Legionella feeleii strains isolated from humans.Thacker WL, Wilkinson HW, Plikaytis BB, Steigerwalt AG, Mayberry WR, Moss CW, Brenner DJJ Clin Microbiol10.1128/jcm.22.1.1-4.19851985Animals, Antigens, Bacterial/immunology, Chick Embryo, DNA, Bacterial, Fatty Acids/analysis, Fluorescent Antibody Technique, Humans, Legionella/analysis/*classification/immunology/isolation & purification, Legionnaires' Disease/*microbiology, Nucleic Acid Hybridization, Pneumonia/*microbiology, Rabbits, Serotyping, Ubiquinone/analysisPathogenicity
Pathogenicity30386929Legionella feeleii: pneumonia or Pontiac fever? Bacterial virulence traits and host immune response.Wang C, Chuai X, Liang MMed Microbiol Immunol10.1007/s00430-018-0571-02018Animals, Cell Line, Cytokines/*metabolism, Humans, Legionella/growth & development/*pathogenicity, Legionnaires' Disease/*microbiology/*pathology, Mice, Polysaccharides, Bacterial/*metabolism/toxicity, Virulence, Virulence Factors/*metabolism/toxicityMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17995Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25316)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25316
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35152Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15724
49704Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29668)https://www.ccug.se/strain?id=29668
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76205Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46315.1StrainInfo: A central database for resolving microbial strain identifiers
119296Curators of the CIPCollection of Institut Pasteur (CIP 103878)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103878