Strain identifier

BacDive ID: 6733

Type strain: Yes

Species: Legionella erythra

Strain Designation: SE-32A-C8

Strain history: CIP <- 1993, R.F. Benson, CDC: strain SE-32A-C8 <- G.W. Gormann

NCBI tax ID(s): 448 (species)

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General

@ref: 7106

BacDive-ID: 6733

DSM-Number: 17644

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, human pathogen

description: Legionella erythra SE-32A-C8 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from water cooling tower.

NCBI tax id

  • NCBI tax id: 448
  • Matching level: species

strain history

@refhistory
7106<- H. E. Müller, Medizinal-Untersuchungsamt Braunschweig <- ATCC <- G. W. Gorman, CDC; SE-32A-C8
67770GIFU 11748 <-- ATCC 35303 <-- G. W. Gorman SE-32A-C8.
119129CIP <- 1993, R.F. Benson, CDC: strain SE-32A-C8 <- G.W. Gormann

doi: 10.13145/bacdive6733.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella erythra
  • full scientific name: Legionella erythra Brenner et al. 1985

@ref: 7106

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella erythra

full scientific name: Legionella erythra Brenner et al. 1985

strain designation: SE-32A-C8

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.996
119129negativerod-shapedyes

colony morphology

@refincubation period
71062-3 days
497033 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7106BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40809MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119129CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7106positivegrowth37mesophilic
40809positivegrowth37mesophilic
49703positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 7106
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.989

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837727897tryptophan-energy source

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377lipase+
68377ornithine decarboxylase-4.1.1.17
119129oxidase+
119129catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119129-+++-+++--++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
7106+-----++--+--
7106-+-----++--+-
7106+------++--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7106water cooling towerSeattleUSAUSANorth America
49703Cooling tower water1981
67770Water in cooling towerSeattle, WAUSAUSANorth America
119129Environment, Water in cooling towerSeattle, WashingtonUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_1469.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_839;97_981;98_1159;99_1469&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: Z32638
  • Sequence Identity:
  • Total samples: 682
  • soil counts: 154
  • aquatic counts: 438
  • animal counts: 55
  • plant counts: 35

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7106yes, in single cases1Risk group (German classification)
1191292Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella erythra strain MDC1578 16S ribosomal RNA gene, partial sequenceJF720410525ena448
20218Legionella erythra 16S rRNA sequenceM360271446ena448
7106L.erythra (SE-32A-C8) gene for 16S ribosomal RNAZ326381454ena448
67770Legionella erythra JCM 7564 gene for 16S ribosomal RNA, partial sequenceLC5040411467ena448

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella erythra SE-32A-C8GCA_001467615scaffoldncbi448
66792Legionella erythra SE-32A-C8GCA_900639845scaffoldncbi448
66792Legionella erythra strain SE-32A-C8448.7wgspatric448
66792Legionella erythra SE-32A-C82695420771draftimg448

GC content

  • @ref: 67770
  • GC-content: 51
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno78.14no
gram-positiveno98.178no
anaerobicno98.412no
aerobicno89.234no
halophileno86.233no
spore-formingno93.274no
glucose-utilyes68.201no
motileyes85.285no
thermophileno95.655yes
glucose-fermentno90.24no

External links

@ref: 7106

culture collection no.: CCUG 29667, DSM 17644, ATCC 35303, CIP 103843, NCTC 11977, JCM 7564

straininfo link

  • @ref: 76203
  • straininfo: 40983

literature

  • topic: Pathogenicity
  • Pubmed-ID: 2715320
  • title: Cellular fatty acid compositions and isoprenoid quinone contents of 23 Legionella species.
  • authors: Lambert MA, Moss CW
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.27.3.465-473.1989
  • year: 1989
  • mesh: Chromatography, Gas, Chromatography, High Pressure Liquid, Fatty Acids/*analysis, Humans, Legionella/*analysis/classification/isolation & purification, Ubiquinone/*analysis
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7106Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17644)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17644
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40809Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15685
49703Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29667)https://www.ccug.se/strain?id=29667
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76203Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40983.1StrainInfo: A central database for resolving microbial strain identifiers
119129Curators of the CIPCollection of Institut Pasteur (CIP 103843)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103843