Strain identifier

BacDive ID: 6726

Type strain: Yes

Species: Legionella pneumophila subsp. fraseri

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.E. Weaver, CDC: strain Los Angeles-1 <- P. Edelstein

NCBI tax ID(s): 91892 (subspecies)

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General

@ref: 3194

BacDive-ID: 6726

DSM-Number: 7514

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella pneumophila subsp. fraseri DSM 7514 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung tissue.

NCBI tax id

  • NCBI tax id: 91892
  • Matching level: subspecies

strain history

@refhistory
3194<- ATCC; ATCC 33156 <- R. E. Weaver; Los Angeles-1 <- P. H. Edelstein;
121909CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.E. Weaver, CDC: strain Los Angeles-1 <- P. Edelstein

doi: 10.13145/bacdive6726.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. fraseri
  • full scientific name: Legionella pneumophila subsp. fraseri Brenner et al. 1989

@ref: 3194

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. fraseri

full scientific name: Legionella pneumophila subsp. fraseri Brenner et al. 1989

type strain: yes

Morphology

cell morphology

  • @ref: 121909
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
31941-2 days
459432 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3194BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
41840MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121909CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
3194positivegrowth37mesophilic
41840positivegrowth37mesophilic
45943positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3194microaerophile
45943microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
3194catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
121909oxidase+
121909catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121909-++--++---++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3194++/--+/----+--+--
3194-++/--+/----+--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3194human lung tissue
45943Human lungLos AngelesUSAUSANorth America
121909Human, Lung

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_897.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_73;98_80;99_897&stattab=map
  • Last taxonomy: Legionella pneumophila subclade
  • 16S sequence: NR_104921
  • Sequence Identity:
  • Total samples: 2103
  • soil counts: 587
  • aquatic counts: 1091
  • animal counts: 330
  • plant counts: 95

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31942Risk group (German classification)
1219092Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella pneumophila strain ATCC33156 16S-23S rRNA intergenic regionAF000656278ena446
20218Legionella pneumophila subsp. fraseri strain Los Angelos-1 16S ribosomal RNA gene, partial sequenceHQ2879021510ena91892
20218Legionella pneumophila subsp. fraseri strain MDC1573 16S ribosomal RNA gene, partial sequenceJF720378524ena91892
3194Legionella pneumophila subsp. fraseri strain Los Angelos-1 16S ribosomal RNA, partial sequenceNR_1049211510nuccore91892

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila ATCC 33156GCA_001582825scaffoldncbi446
66792Legionella pneumophila strain ATCC 33156446.178wgspatric446
66792Legionella pneumophila fraseri ATCC 331562671181071draftimg91892
66792Legionella pneumophila subsp. fraseri ATCC 33156GCA_000586315scaffoldncbi91892

GC content

  • @ref: 3194
  • GC-content: 39.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno76.848no
gram-positiveno98.445no
anaerobicno98.49no
aerobicno91.549yes
halophileno89.141no
spore-formingno93.861no
glucose-utilyes67.485no
motileyes78.608no
thermophileno97.92yes
glucose-fermentno86.286no

External links

@ref: 3194

culture collection no.: DSM 7514, ATCC 33156, WDCM 00180, CCUG 13398, NCTC 11233, Los Angeles-1, CIP 103858, CECT 8343

straininfo link

  • @ref: 76196
  • straininfo: 40362

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3194Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7514)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7514
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41840Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15702
45943Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 13398)https://www.ccug.se/strain?id=13398
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76196Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40362.1StrainInfo: A central database for resolving microbial strain identifiers
121909Curators of the CIPCollection of Institut Pasteur (CIP 103858)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103858