Strain identifier

BacDive ID: 6699

Type strain: Yes

Species: Apilactobacillus ozensis

Strain Designation: Mizu2-1

Strain history: S. Kawasaki Mizu2-1.

NCBI tax ID(s): 866801 (species)

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General

@ref: 17392

BacDive-ID: 6699

DSM-Number: 23829

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Apilactobacillus ozensis Mizu2-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Inula ciliaris var. glandulosa, a chrysanthemum.

NCBI tax id

  • NCBI tax id: 866801
  • Matching level: species

strain history

@refhistory
17392<- S. Kawasaki, Dept. Bio-Science, Tokyo Univ. Agriculture, Japan; JCM 17196 <- S. Kawasaki, Dept. Bio-Science, Tokyo Univ. Agriculture, Japan
67770S. Kawasaki Mizu2-1.

doi: 10.13145/bacdive6699.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Apilactobacillus
  • species: Apilactobacillus ozensis
  • full scientific name: Apilactobacillus ozensis (Kawasaki et al. 2011) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus ozensis

@ref: 17392

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Apilactobacillus

species: Apilactobacillus ozensis

full scientific name: Apilactobacillus ozensis (Kawasaki et al. 2011) Zheng et al. 2020

strain designation: Mizu2-1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
125438no91.5
12543892.777positive

Culture and growth conditions

culture medium

  • @ref: 17392
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperature
17392positivegrowth30
67770positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17392microaerophile
125439obligate aerobe98.5

spore formation

  • @ref: 125438
  • spore formation: no
  • confidence: 90.759

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
17392-----------++/--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
17392Inula ciliaris var. glandulosa, a chrysanthemumInula ciliaris var. glandulosaGunma prefecture, Oze National ParkJapanJPNAsia
67770Mountain flowersOze National ParkJapanJPNAsia

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_8228.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_3783;97_4664;98_5990;99_8228&stattab=map
  • Last taxonomy: Apilactobacillus ozensis subclade
  • 16S sequence: LC519864
  • Sequence Identity:
  • Total samples: 2423
  • soil counts: 51
  • aquatic counts: 88
  • animal counts: 2085
  • plant counts: 199

Safety information

risk assessment

  • @ref: 17392
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
17392Lactobacillus ozensis gene for 16S rRNA, partial sequence, strain: Mizu2-1AB5725881541nuccore1423781
67770Lactobacillus ozensis JCM 17196 gene for 16S rRNA, partial sequenceLC5198641511nuccore866801

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus ozensis DSM 23829 = JCM 171961423781.4wgspatric1423781
66792Lactobacillus ozensis DSM 23829 = JCM 171961423781.3wgspatric1423781
66792Apilactobacillus ozensis DSM 238292660238120draftimg1423781
66792Apilactobacillus ozensis JCM 171962675903667draftimg1423781
67770Apilactobacillus ozensis DSM 23829 = JCM 17196GCA_001435995scaffoldncbi1423781
67770Apilactobacillus ozensis DSM 23829 = JCM 17196GCA_001311485contigncbi1423781

GC content

@refGC-contentmethod
1739241high performance liquid chromatography (HPLC)
6777031.9genome sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes92.777no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no84.239yes
125438spore-formingspore-formingAbility to form endo- or exosporesno90.759no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no88.299yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno91.557no
125438motile2+flagellatedAbility to perform flagellated movementno91.5no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno74.9
125439BacteriaNetmotilityAbility to perform movementyes53.2
125439BacteriaNetgram_stainReaction to gram-stainingpositive73
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe98.5

External links

@ref: 17392

culture collection no.: DSM 23829, JCM 17196, LMG 29536

straininfo link

  • @ref: 76173
  • straininfo: 379062

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21075903Lactobacillus ozensis sp. nov., isolated from mountain flowers.Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Niimura YInt J Syst Evol Microbiol10.1099/ijs.0.027847-02010Aerobiosis, Anaerobiosis, Bacterial Proteins/genetics, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fermentation, Flowers/*microbiology, Heterotrophic Processes, Japan, Lactobacillus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Enzymology29051101Molecular characterization and bioinformatics studies of a lipase from Bacillus thermoamylovorans BHK67.Sharma A, Meena KR, Kanwar SSInt J Biol Macromol10.1016/j.ijbiomac.2017.10.0922017Amino Acid Sequence, Anions, Bacillus/*enzymology, Chromatography, Ion Exchange, Computational Biology/*methods, Dynamic Light Scattering, Enzyme Stability, Extracellular Space/enzymology, Hydrogen-Ion Concentration, Kinetics, Lipase/chemistry/*genetics/isolation & purification, Models, Molecular, Peptide Mapping, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA, Solvents, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Structural Homology, Protein, Substrate Specificity, TemperaturePhylogeny
Phylogeny29353462Lactobacillus kosoi sp. nov., a fructophilic species isolated from koso, a Japanese sugar-vegetable fermented beverage.Chiou TY, Suda W, Oshima K, Hattori M, Matsuzaki C, Yamamoto K, Takahashi TAntonie Van Leeuwenhoek10.1007/s10482-018-1019-72018Base Composition, Beverages/analysis/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Fermentation, Fermented Foods/analysis/*microbiology, Fructose/*metabolism, Hydrogen-Ion Concentration, Japan, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sugars/chemistry/*metabolism, Vegetables/chemistry/*microbiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17392Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23829)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23829
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76173Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID379062.1StrainInfo: A central database for resolving microbial strain identifiers
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1