Strain identifier

BacDive ID: 6696

Type strain: Yes

Species: Fructilactobacillus florum

Strain Designation: F9-1

Strain history: A. Endo F9-1.

NCBI tax ID(s): 640331 (species)

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General

@ref: 16464

BacDive-ID: 6696

DSM-Number: 22689

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Fructilactobacillus florum F9-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from peony .

NCBI tax id

  • NCBI tax id: 640331
  • Matching level: species

strain history

@refhistory
16464<- A. Endo, Dept. Microbiology, Stellenbosch Univ., South Africa; F9-1
67770A. Endo F9-1.

doi: 10.13145/bacdive6696.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructilactobacillus
  • species: Fructilactobacillus florum
  • full scientific name: Fructilactobacillus florum (Endo et al. 2010) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus florum

@ref: 16464

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Fructilactobacillus

species: Fructilactobacillus florum

full scientific name: Fructilactobacillus florum (Endo et al. 2010) Zheng et al. 2020

strain designation: F9-1

type strain: yes

Morphology

cell morphology

  • @ref: 29704
  • gram stain: positive
  • cell length: 1.5-7 µm
  • cell width: 0.8 µm
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 29704
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16464
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11; with strain-specific modifications) Composition: Glucose 20.0 g/l Fructose 10.0 g/l Meat extract 10.0 g/l Casein peptone 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16464positivegrowth30mesophilic
29704positivegrowth15psychrophilic
29704positiveoptimum15psychrophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
29704positivegrowth04-08acidophile
29704positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16464microaerophile
29704facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
29704NaClpositivegrowth5 %
29704NaClpositiveoptimum5 %

observation

  • @ref: 29704
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2970428757fructose+carbon source
2970417234glucose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

  • @ref: 29704
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16464-----------++----------------------------------+--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
16464peony (Paeonia suffruticosa)Paeonia suffruticosaWestern Cape, StellenboschSouth AfricaZAFAfrica
67770Peony and bietou flowersStellenbosch and Hermanus area, Western CapeSouth AfricaZAFAfrica

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Shrub (Scrub)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3236.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1683;97_2016;98_2478;99_3236&stattab=map
  • Last taxonomy: Fructilactobacillus florum subclade
  • 16S sequence: LC483564
  • Sequence Identity:
  • Total samples: 2601
  • soil counts: 76
  • aquatic counts: 473
  • animal counts: 1951
  • plant counts: 101

Safety information

risk assessment

  • @ref: 16464
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16464Lactobacillus florum gene for 16S rRNA, partial sequenceAB4980451507ena1423745
67770Lactobacillus florum gene for 16S ribosomal RNA, partial sequenceLC4835641516ena640331

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus florum DSM 22689 = JCM 160351423745.4wgspatric1423745
66792Lactobacillus florum DSM 22689 = JCM 160351423745.3wgspatric1423745
66792Fructilactobacillus florum DSM 226892657244926draftimg1423745
66792Fructilactobacillus florum JCM 160352728369675draftimg1423745
67770Fructilactobacillus florum DSM 22689 = JCM 16035GCA_001436645contigncbi1423745
67770Fructilactobacillus florum DSM 22689 = JCM 16035GCA_001311215contigncbi1423745

GC content

@refGC-contentmethod
1646442high performance liquid chromatography (HPLC)
2970442
6777041.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.364yes
gram-positiveyes95.301no
anaerobicno90.284yes
halophileyes89.917no
spore-formingno97.41no
glucose-utilyes88.806yes
thermophileno98.31yes
aerobicno95.439yes
flagellatedno98.809yes
glucose-fermentyes80.221no

External links

@ref: 16464

culture collection no.: DSM 22689, JCM 16035, NRIC 0771

straininfo link

  • @ref: 76170
  • straininfo: 371014

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19965998Lactobacillus florum sp. nov., a fructophilic species isolated from flowers.Endo A, Futagawa-Endo Y, Sakamoto M, Kitahara M, Dicks LMTInt J Syst Evol Microbiol10.1099/ijs.0.019067-02009Bacterial Typing Techniques, Carbohydrate Metabolism, Carboxylic Acids/metabolism, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ethanol/metabolism, Flowers/*microbiology, Fructose/*metabolism, Genotype, Lactobacillus/*classification/genetics/isolation & purification/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, South AfricaMetabolism
Phylogeny22137250Identification and characterization of Lactobacillus florum strains isolated from South African grape and wine samples.Mtshali PS, Divol B, du Toit MInt J Food Microbiol10.1016/j.ijfoodmicro.2011.10.0232011Beer/microbiology, DNA Primers, DNA, Ribosomal/genetics/metabolism, Hydrolases, Lactobacillus/enzymology/*genetics/isolation & purification, Phylogeny, Polymerase Chain Reaction, South Africa, Species Specificity, Vitis/*microbiology, Wine/*microbiologyEnzymology
Phylogeny27902183Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian jasmine).Techo S, Miyashita M, Shibata C, Tanaka N, Wisetkhan P, Visessanguan W, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0015472016Bacillus coagulans, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Flowers/*microbiology, Jasminum/*microbiology, Lactic Acid/biosynthesis, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, ThailandTranscriptome
34975780Apple Pomace as a Sustainable Substrate in Sourdough Fermentation.Martau GA, Teleky BE, Ranga F, Pop ID, Vodnar DCFront Microbiol10.3389/fmicb.2021.7420202021
36266426Biofunctional soy-based sourdough for improved rheological properties during storage.Teleky BE, Martau GA, Ranga F, Pop ID, Vodnar DCSci Rep10.1038/s41598-022-22551-z2022*Flour/analysis, *Bread/analysis, Triticum/chemistry, Soybean Proteins/chemistry, Powders, Glutens/chemistry, Starch, Water/chemistry

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16464Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22689)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22689
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29704Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2608728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76170Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID371014.1StrainInfo: A central database for resolving microbial strain identifiers