Strain identifier

BacDive ID: 6664

Type strain: No

Species: Ligilactobacillus salivarius

Strain Designation: HO268, HO 268

Strain history: CIP <- 1988, NCFB, Lactobacillus salivarius subsp. salicinius <- 1961, M.E. Sharpe, Reading, UK: strain HO 268

NCBI tax ID(s): 1624 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 8904

BacDive-ID: 6664

DSM-Number: 20554

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Ligilactobacillus salivarius HO268 is a microaerophile, mesophilic bacterium that was isolated from saliva.

NCBI tax id

  • NCBI tax id: 1624
  • Matching level: species

strain history

@refhistory
8904<- ATCC <- M. Rogosa, HO268
67770ATCC 11742 <-- M. Rogosa HO268.
122388CIP <- 1988, NCFB, Lactobacillus salivarius subsp. salicinius <- 1961, M.E. Sharpe, Reading, UK: strain HO 268

doi: 10.13145/bacdive6664.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Ligilactobacillus
  • species: Ligilactobacillus salivarius
  • full scientific name: Ligilactobacillus salivarius (Rogosa et al. 1953) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus salivarius subsp. salicinius
    20215Lactobacillus salivarius

@ref: 8904

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Ligilactobacillus

species: Ligilactobacillus salivarius

full scientific name: Ligilactobacillus salivarius (Rogosa et al. 1953) Zheng et al. 2020

strain designation: HO268, HO 268

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.634
69480100positive
122388nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8904MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34120MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122388CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8904positivegrowth37mesophilic
34120positivegrowth37mesophilic
54803positivegrowth37mesophilic
67770positivegrowth37mesophilic
122388positivegrowth25-45
122388nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
54803microaerophile
122388facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.883

murein

  • @ref: 8904
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol+builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122388nitrate-reduction17632
122388nitrite-reduction16301
122388nitrate+respiration17632

metabolite production

  • @ref: 122388
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 122388
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
122388alcohol dehydrogenase-1.1.1.1
122388catalase-1.11.1.6
122388lysine decarboxylase-4.1.1.18
122388ornithine decarboxylase-4.1.1.17
122388urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122388-+--------++++------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8904----------++++----++--+-+++-+++++--+----------+---
122388----------+++----++--+-+++-+++++--+----------+---

Isolation, sampling and environmental information

isolation

@refsample type
8904saliva
54803Human saliva
67770Saliva
122388Human, Saliva

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Oral cavity and Airways
  • Cat3: #Saliva

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89041Risk group (German classification)
1223881Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus salivarius subsp. salicinius 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF080102479ena1624
20218Lactobacillus salivarius salicinius 16S ribosomal RNAM590541512ena1624

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ligilactobacillus salivarius BCRC 12574GCA_002735985chromosomencbi1624
66792Ligilactobacillus salivarius DSM 20554GCA_002079585scaffoldncbi1624
66792Lactobacillus salivarius strain BCRC 125741624.125plasmidpatric1624
66792Lactobacillus salivarius strain BCRC 125741624.112completepatric1624
66792Lactobacillus salivarius strain BCRC 125741624.126plasmidpatric1624
66792Lactobacillus salivarius strain BCRC 125741624.124plasmidpatric1624
66792Lactobacillus salivarius strain DSM 205541624.58wgspatric1624
66792Ligilactobacillus salivarius BCRC 125742898124533completeimg1624

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno78no
motileno96.064no
gram-positiveyes95.412no
anaerobicno62.808no
aerobicno96.705yes
halophileyes84.822no
spore-formingno95.247no
thermophileno97.545no
glucose-utilyes92.327no
flagellatedno98.294no
glucose-fermentyes88.351no

External links

@ref: 8904

culture collection no.: DSM 20554, ATCC 11742, NCDO 1555, CCUG 39464, JCM 1150, NRIC 1072, BCRC 12574, CECT 4062, CIP 103155, KCTC 3600, LMG 9476, NBRC 102160, NCFB 1555, NRRL B-1950

straininfo link

  • @ref: 76138
  • straininfo: 3365

literature

  • Pubmed-ID: 29167259
  • title: Whole-Genome Sequencing of Lactobacillus salivarius Strains BCRC 14759 and BCRC 12574.
  • authors: Chiu SH, Chen CC, Wang LT, Huang L
  • journal: Genome Announc
  • DOI: 10.1128/genomeA.01336-17
  • year: 2017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8904Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20554)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20554
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34120Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14921
54803Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39464)https://www.ccug.se/strain?id=39464
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76138Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3365.1StrainInfo: A central database for resolving microbial strain identifiers
122388Curators of the CIPCollection of Institut Pasteur (CIP 103155)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103155