Strain identifier

BacDive ID: 6654

Type strain: No

Species: Levilactobacillus brevis

Strain Designation: D 13

Strain history: CIP <- 1992, DSM <- W. Back: strain D 13, Lactobacillus frigidus

NCBI tax ID(s): 1580 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2551

BacDive-ID: 6654

DSM-Number: 6235

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Levilactobacillus brevis D 13 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from spoilt beer.

NCBI tax id

  • NCBI tax id: 1580
  • Matching level: species

strain history

@refhistory
2551<- W. Back, D 13. (Lactobacillus frigidus)
117043CIP <- 1992, DSM <- W. Back: strain D 13, Lactobacillus frigidus

doi: 10.13145/bacdive6654.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Levilactobacillus
  • species: Levilactobacillus brevis
  • full scientific name: Levilactobacillus brevis (Orla-Jensen 1919) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus brevis
    20215Betabacterium breve

@ref: 2551

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Levilactobacillus

species: Levilactobacillus brevis

full scientific name: Levilactobacillus brevis (Orla-Jensen 1919) Zheng et al. 2020

strain designation: D 13

type strain: no

Morphology

cell morphology

  • @ref: 117043
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2551MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
41669MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
117043CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
117043CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
2551positivegrowth30mesophilic
41669positivegrowth37mesophilic
117043positivegrowth10-37
117043nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117043
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117043nitrate-reduction17632
117043nitrite-reduction16301
117043nitrate+respiration17632

metabolite tests

  • @ref: 117043
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
117043oxidase-
117043alcohol dehydrogenase+1.1.1.1
117043catalase-1.11.1.6
117043lysine decarboxylase-4.1.1.18
117043ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117043--++-+++--+++++++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
2551----+++---+++------------+--+---------------------
2551----+++---+++--------++--+--+-+----------------+/---
2551----+++/----+/-++/----------+/---+--+/--+-------------------
2551----+++---+++---------+-------+----------------+--
2551----+++---+++-------++---+--+-+----------------+/-+/--

Isolation, sampling and environmental information

isolation

@refsample type
2551spoilt beer
117043Other, Spoilt beer

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Environmental#Microbial community
#Engineered#Food production#Beverage

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
25511Risk group (German classification)
1170431Risk group (French classification)

External links

@ref: 2551

culture collection no.: DSM 6235, CCM 7932, CIP 103474

straininfo link

  • @ref: 76128
  • straininfo: 8909

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity32179950Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.Munford ARG, Chaves RD, Granato D, Sant'Ana ASAppl Microbiol Biotechnol10.1007/s00253-020-10534-x2020Beer/microbiology, Chlorine Compounds/*pharmacology, *Fermentation, Hydrogen-Ion Concentration, Lactobacillus brevis/drug effects/*physiology, *Microbial Viability, Oxides/*pharmacology, Phosphoric Acids/*pharmacology, Saccharomyces cerevisiae/drug effects/*physiology, Temperature
32529375Correction to: Modeling the inactivation of Lactobacillus brevis DSM 6235 and retaining the viability of brewing pitching yeast submitted to acid and chlorine washing.Munford ARG, Chaves RD, Granato D, Sant'Ana ASAppl Microbiol Biotechnol10.1007/s00253-020-10738-12020
Enzymology32539960Inactivation kinetics of beer spoilage bacteria (Lactobacillus brevis, Lactobacillus casei, and Pediococcus damnosus) during acid washing of brewing yeast.Munford ARG, Chaves RD, Sant'Ana ASFood Microbiol10.1016/j.fm.2020.1035132020Beer/*microbiology, Bioreactors/microbiology, Fermentation, Food Contamination/prevention & control, Food Microbiology, Hydrogen-Ion Concentration, Kinetics, Lactobacillaceae/classification/*drug effects/growth & development/metabolism, Phosphoric Acids/*pharmacology, Yeasts/*growth & development/metabolismBiotechnology

Reference

@idauthorscataloguedoi/urltitle
2551Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6235)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6235
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41669Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15275
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
76128Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8909.1StrainInfo: A central database for resolving microbial strain identifiers
117043Curators of the CIPCollection of Institut Pasteur (CIP 103474)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103474