Strain identifier

BacDive ID: 6627

Type strain: Yes

Species: Lactobacillus kitasatonis

Strain Designation: Ch-J-2-11, S1-99

Strain history: CIP <- 2003, JCM <- T. Mitsuoka: strain Ch-J-2-11

NCBI tax ID(s): 1423767 (strain), 237446 (species)

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General

@ref: 6496

BacDive-ID: 6627

DSM-Number: 16761

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped

description: Lactobacillus kitasatonis Ch-J-2-11 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from chicken, intestine.

NCBI tax id

NCBI tax idMatching level
1423767strain
237446species

strain history

@refhistory
6496<- JCM <- T. Mitsuoka, strain Ch-J-2-11
67770T. Mitsuoka Ch-J-2-11.
67771<- JCM <- IPCR, S1-99 <- T Mitsuoka, Ch-J-2-11
120450CIP <- 2003, JCM <- T. Mitsuoka: strain Ch-J-2-11

doi: 10.13145/bacdive6627.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus kitasatonis
  • full scientific name: Lactobacillus kitasatonis Mukai et al. 2003

@ref: 6496

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus kitasatonis

full scientific name: Lactobacillus kitasatonis Mukai et al. 2003

strain designation: Ch-J-2-11, S1-99

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stainconfidence
67771rod-shapedno
67771positive
69480no93.956
69480positive100
120450rod-shapednopositive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6496MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38264MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120450CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
6496positivegrowth37mesophilic
38264positivegrowth37mesophilic
60044positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
120450positivegrowth30-45
120450nogrowth10psychrophilic
120450nogrowth15psychrophilic
120450nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6496microaerophile
60044microaerophile
67771microaerophile

spore formation

@refspore formationconfidence
67771no
69481no100
69480no99.999

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120450D-fructose-degradation15824
120450D-glucose-degradation17634
120450D-mannose-degradation16024
120450lactose-degradation17716
120450maltose-degradation17306
120450sucrose-degradation17992
120450nitrate-reduction17632
120450nitrite-reduction16301
120450nitrate+respiration17632

antibiotic resistance

  • @ref: 120450
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 120450
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120450oxidase-
120450alcohol dehydrogenase-1.1.1.1
120450catalase-1.11.1.6
120450lysine decarboxylase-4.1.1.18
120450ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120450--+--+-+--+++--+++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
6496-----------+++--------+--+-+/-+--+----+/--------------
6496-----------+++--------+--+-++--+-------+----------
120450---------++++/---------++/-+/-+/-+/-++--+----+/---+----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
6496chicken, intestineJapanJPNAsia
60044Chicken intestineJapanJPNAsia
67770Chicken intestine
67771From chicken intestineJapanJPNAsia
120450Chicken intestineJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Gastrointestinal tract#Stomach

taxonmaps

  • @ref: 69479
  • File name: preview.99_7369.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_146;97_157;98_175;99_7369&stattab=map
  • Last taxonomy: Lactobacillus
  • 16S sequence: LC064885
  • Sequence Identity:
  • Total samples: 6159
  • soil counts: 44
  • aquatic counts: 67
  • animal counts: 5968
  • plant counts: 80

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64961Risk group (German classification)
1204501Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus kitasatonis gene for 16S rRNA, partial sequence, strain: JCM 1039AB289190648ena237446
6496Lactobacillus kitasatonis gene for 16S ribosomal RNA, partial sequenceAB1076381506ena237446
67770Lactobacillus kitasatonis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1039LC0648851464ena237446

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus kitasatonis DSM 16761 = JCM 10391423767.3wgspatric1423767
66792Lactobacillus kitasatonis JCM 10391236941.3wgspatric1423767
66792Lactobacillus kitasatonis DSM 167612660238082draftimg1423767
66792Lactobacillus kitasatonis JCM 10392565956592draftimg1423767
67770Lactobacillus kitasatonis DSM 16761 = JCM 1039GCA_001434435scaffoldncbi1423767
67771Lactobacillus kitasatonis DSM 16761 = JCM 1039GCA_000615285contigncbi1423767

GC content

@refGC-contentmethod
649637
6777137.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.762no
gram-positiveyes95.927yes
anaerobicyes61.637yes
halophileyes85.634no
spore-formingno97.39yes
thermophileno99.538yes
glucose-utilyes92.323no
aerobicno97.937yes
flagellatedno97.842yes
glucose-fermentyes89.888no

External links

@ref: 6496

culture collection no.: CCUG 53874, CIP 108218, DSM 16761, JCM 1039, KCTC 3155, BCRC 17387, LMG 23133

straininfo link

  • @ref: 76101
  • straininfo: 63137

literature

  • topic: Phylogeny
  • Pubmed-ID: 14657145
  • title: Lactobacillus kitasatonis sp. nov., from chicken intestine.
  • authors: Mukai T, Arihara K, Ikeda A, Nomura K, Suzuki F, Ohori H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02815-0
  • year: 2003
  • mesh: Animals, Chickens/*microbiology, DNA, Bacterial/genetics, Genome, Bacterial, Glucose/metabolism, Intestines/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Lactobacillus acidophilus/classification/genetics, Molecular Sequence Data, *Phylogeny, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6496Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16761)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16761
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38264Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5816
60044Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53874)https://www.ccug.se/strain?id=53874
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76101Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID63137.1StrainInfo: A central database for resolving microbial strain identifiers
120450Curators of the CIPCollection of Institut Pasteur (CIP 108218)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108218