Strain identifier

BacDive ID: 6602

Type strain: Yes

Species: Latilactobacillus sakei subsp. carnosus

Strain Designation: R 14b/a

Strain history: CIP <- 1998, CCUG <- G. Klein and G. Reuter, Berlin, Germany

NCBI tax ID(s): 525363 (strain), 214325 (subspecies)

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General

@ref: 6043

BacDive-ID: 6602

DSM-Number: 15831

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Latilactobacillus sakei subsp. carnosus R 14b/a is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from fermented meat produkt.

NCBI tax id

NCBI tax idMatching level
525363strain
214325subspecies

strain history

@refhistory
6043<- JCM <- G. Klein <- G. Reuter
67770CIP 105422 <-- CCUG 31331 <-- G. Klein and G. Reuter.
67771<- CCUG <- G Klein and G Reuter
122356CIP <- 1998, CCUG <- G. Klein and G. Reuter, Berlin, Germany

doi: 10.13145/bacdive6602.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Latilactobacillus
  • species: Latilactobacillus sakei subsp. carnosus
  • full scientific name: Latilactobacillus sakei subsp. carnosus (Torriani et al. 1996) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus sake subsp. carnosus
    20215Lactobacillus curvatus subsp. melibiosus
    20215Lactobacillus sakei subsp. carnosus

@ref: 6043

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Latilactobacillus

species: Latilactobacillus sakei subsp. carnosus

full scientific name: Latilactobacillus sakei subsp. carnosus (Torriani et al. 1996) Zheng et al. 2020

strain designation: R 14b/a

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidencecell shape
67771positive
69480no92.66
69480positive100
122356positivenorod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6043MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40506MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122356CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
6043positivegrowth37mesophilic
40506positivegrowth37mesophilic
50526positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
122356positivegrowth10-41
122356nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6043microaerophile
50526anaerobe
50526microaerophile
67771facultative anaerobe
122356facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.99

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122356nitrate-reduction17632
122356nitrite-reduction16301
122356nitrate+respiration17632

metabolite tests

  • @ref: 122356
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
122356oxidase-
122356alcohol dehydrogenase-1.1.1.1
122356catalase-1.11.1.6
122356lysine decarboxylase-4.1.1.18
122356ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122356-+---++---+++-------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
6043----++----++++--------+--+--+-+++------+-------+--
122356---++----++++--------+--+/---+-+++------+-------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6043fermented meat produktBerlinGermanyDEUEurope
50526Raw sausage
67770Fermented meat product
67771From fermented meat product(raw sausage)BerlinGermanyDEUEurope
122356Raw sausageGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_668.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_411;97_466;98_537;99_668&stattab=map
  • Last taxonomy: Latilactobacillus
  • 16S sequence: LC145559
  • Sequence Identity:
  • Total samples: 26696
  • soil counts: 1455
  • aquatic counts: 2674
  • animal counts: 21518
  • plant counts: 1049

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
60431Risk group (German classification)
1223561Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus sakei subsp. carnosus strain CCUG 31331 16S small subunit ribosomal RNA gene, partial sequenceAY2048921414ena214325
20218Lactobacillus sakei subsp. carnosus gene for 16S rRNA, partial sequence, strain: JCM 11031AB289288658ena214325
67770Lactobacillus sakei subsp. carnosus gene for 16S ribosomal RNA, partial sequence, strain: JCM 11031LC1455591513ena214325

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Latilactobacillus sakei subsp. carnosus NBRC 107868GCA_007992315contigncbi214325
66792Lactobacillus sakei subsp. carnosus DSM 15831525363.3wgspatric525363
66792Lactobacillus sakei subsp. carnosus strain NBRC 107868214325.5wgspatric214325
67770Latilactobacillus sakei subsp. carnosus DSM 15831GCA_001435715scaffoldncbi525363

GC content

@refGC-contentmethod
604342-44
6777042high performance liquid chromatography (HPLC)
6777041genome sequence analysis
6777142.0high performance liquid chromatography (HPLC)
6777141.0genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveyes96.415yes
anaerobicno93.777no
halophileyes93.539no
spore-formingno93.82no
glucose-utilyes91.555no
thermophileno99.429yes
motileno95.126no
aerobicno97.194yes
flagellatedno98.657no
glucose-fermentyes86.596no

External links

@ref: 6043

culture collection no.: DSM 15831, CCUG 31331, CIP 105422, JCM 11031, KCTC 3802, BCRC 17500, CECT 5766, KCTC 5053, LMG 17302, NBRC 107868

straininfo link

  • @ref: 76077
  • straininfo: 9261

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6043Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15831)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15831
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40506Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17440
50526Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31331)https://www.ccug.se/strain?id=31331
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76077Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9261.1StrainInfo: A central database for resolving microbial strain identifiers
122356Curators of the CIPCollection of Institut Pasteur (CIP 105422)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105422