Strain identifier

BacDive ID: 6588

Type strain: Yes

Species: Latilactobacillus fuchuensis

Strain Designation: B5M10

Strain history: CIP <- 2002, JCM <- 2001, Y. Kato <- H. Hayashidani: strain B5M10

NCBI tax ID(s): 164393 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5311

BacDive-ID: 6588

DSM-Number: 14340

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, psychrophilic, rod-shaped

description: Latilactobacillus fuchuensis B5M10 is an anaerobe, psychrophilic, rod-shaped bacterium that was isolated from vacuum-packaged beef.

NCBI tax id

  • NCBI tax id: 164393
  • Matching level: species

strain history

@refhistory
5311<- Y. Kato <- H. Hayashida; B5M10
67770Y. Kato B5M10 <-- H. Hayashidani.
67771<- JCM <- Y Kato B5M10 <- H Hayashidani
119476CIP <- 2002, JCM <- 2001, Y. Kato <- H. Hayashidani: strain B5M10

doi: 10.13145/bacdive6588.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Latilactobacillus
  • species: Latilactobacillus fuchuensis
  • full scientific name: Latilactobacillus fuchuensis (Sakala et al. 2002) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus fuchuensis

@ref: 5311

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Latilactobacillus

species: Latilactobacillus fuchuensis

full scientific name: Latilactobacillus fuchuensis (Sakala et al. 2002) Zheng et al. 2020

strain designation: B5M10

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771rod-shapedno
67771curved-shaped
67771positive
119476rod-shapednopositive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5311MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39718MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119476CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
5311positivegrowth20psychrophilic
39718positivegrowth20psychrophilic
57526positivegrowth30mesophilic
67770positivegrowth20psychrophilic
67771positivegrowth20psychrophilic
119476positivegrowth15-30
119476nogrowth10psychrophilic
119476nogrowth37mesophilic
119476nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57526anaerobe
67771facultative anaerobe
119476facultative anaerobe

spore formation

  • @ref: 67771
  • spore formation: no

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119476nitrate-reduction17632
119476nitrite-reduction16301
119476nitrate+respiration17632

antibiotic resistance

  • @ref: 119476
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 119476
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119476oxidase-
119476alcohol dehydrogenase-1.1.1.1
119476catalase-1.11.1.6
119476lysine decarboxylase-4.1.1.18
119476ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119476-+---++---++----++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
5311-----++---++++-+------++++++++--+---------+-------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5311vacuum-packaged beefJapanJPNAsia
57526Vacuum-packaged refrigerated beef
67770Vacuum-packaged refrigerated beef
67771From vacuum-packaged refrigerated beefJapanJPNAsia
119476Food, Beef, vacuum packageJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_7993.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_411;97_466;98_5829;99_7993&stattab=map
  • Last taxonomy: Latilactobacillus fuchuensis subclade
  • 16S sequence: JX986973
  • Sequence Identity:
  • Total samples: 6808
  • soil counts: 436
  • aquatic counts: 968
  • animal counts: 4850
  • plant counts: 554

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53111Risk group (German classification)
1194761Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus fuchuensis gene for 16S rRNA, partial sequence, strain: JCM 11249AB289120641ena164393
20218Lactobacillus fuchuensis gene for 16S ribosomal RNA, partial sequence, strain: B5M10 (=JCM 11249, =DSM 14340)AB3708751495ena164393
5311Lactobacillus fuchuensis gene for 16S rRNAAB0634791329ena164393
67770Lactobacillus fuchuensis strain LMG 21669 16S ribosomal RNA gene, partial sequenceJX9869731524ena164393
67771Lactobacillus fuchuensis JCM 11249 gene for 16S ribosomal RNA, partial sequenceLC4807991508ena164393

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus fuchuensis DSM 14340 = JCM 112491423747.3wgspatric1423747
66792Lactobacillus fuchuensis JCM 112491236949.3wgspatric1423747
66792Lactobacillus fuchuensis DSM 143402700989144draftimg1423747
66792Lactobacillus fuchuensis JCM 112492585427876draftimg1423747
67770Latilactobacillus fuchuensis DSM 14340 = JCM 11249GCA_001435065scaffoldncbi1423747
67771Latilactobacillus fuchuensis DSM 14340 = JCM 11249GCA_000615805contigncbi1423747

GC content

@refGC-contentmethod
531141.4high performance liquid chromatography (HPLC)
6777141.8genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.071no
gram-positiveyes94.982yes
anaerobicno95.612no
halophileyes89.291no
spore-formingno93.503no
thermophileno99.475yes
glucose-utilyes91.284no
aerobicno97.849yes
flagellatedno98.478yes
glucose-fermentyes86.838no

External links

@ref: 5311

culture collection no.: DSM 14340, JCM 11249, CCUG 47133, CCM 7144, CIP 107633, KCTC 3797, LMG 21669, NCIMB 14046, CIP 07633

straininfo link

  • @ref: 76062
  • straininfo: 265464

literature

  • topic: Phylogeny
  • Pubmed-ID: 12148620
  • title: Lactobacillus fuchuensis sp. nov., isolated from vacuum-packaged refrigerated beef.
  • authors: Sakala RM, Kato Y, Hayashidani H, Murakami M, Kaneuchi C, Ogawa M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-4-1151
  • year: 2002
  • mesh: Animals, Bacterial Proteins/chemistry, Bacterial Typing Techniques, Cattle, DNA, Ribosomal/analysis, Food Microbiology, Food Packaging/methods, Genes, rRNA, Lactobacillus/*classification/genetics/isolation & purification/metabolism, Meat/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Refrigeration, Sequence Analysis, DNA, Vacuum
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5311Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14340)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14340
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39718Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5090
57526Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47133)https://www.ccug.se/strain?id=47133
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76062Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265464.1StrainInfo: A central database for resolving microbial strain identifiers
119476Curators of the CIPCollection of Institut Pasteur (CIP 107633)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107633