Strain identifier

BacDive ID: 6583

Type strain: Yes

Species: Lacticaseibacillus manihotivorans

Strain history: CIP <- 1999, J. Morlon-Guyot, IRD (ex ORSTOM), Montpellier, France: strain OND 32

NCBI tax ID(s): 88233 (species)

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General

@ref: 5005

BacDive-ID: 6583

DSM-Number: 13343

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lacticaseibacillus manihotivorans CCUG 42894 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from cassava sour starch fermentation.

NCBI tax id

  • NCBI tax id: 88233
  • Matching level: species

strain history

@refhistory
5005<- LMG <- J. Morlon-Guyot <- M. Raimbault; OND 32
67770CIP 105851 <-- J. Marlon-Guyot OND32.
120776CIP <- 1999, J. Morlon-Guyot, IRD (ex ORSTOM), Montpellier, France: strain OND 32

doi: 10.13145/bacdive6583.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus manihotivorans
  • full scientific name: Lacticaseibacillus manihotivorans (Morlon-Guyot et al. 1998) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus manihotivorans

@ref: 5005

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus manihotivorans

full scientific name: Lacticaseibacillus manihotivorans (Morlon-Guyot et al. 1998) Zheng et al. 2020

type strain: yes

Morphology

cell morphology

  • @ref: 120776
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5005MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34638MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120776CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
5005positivegrowth30mesophilic
34638positivegrowth30mesophilic
67770positivegrowth30mesophilic
120776positivegrowth10-45

Physiology and metabolism

oxygen tolerance

  • @ref: 120776
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 5005
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120776nitrate-reduction17632
120776nitrite-reduction16301
120776nitrate+respiration17632

metabolite tests

  • @ref: 120776
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
120776oxidase-
120776alcohol dehydrogenase-1.1.1.1
120776catalase-1.11.1.6
120776lysine decarboxylase-4.1.1.18
120776ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120776-+---++---++---++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
5005----------++++-+/------++-+/-++++-+++--+---+/-----------
120776---------++++-+-----+++/--+++++/-+++--+++-+/-----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5005cassava sour starch fermentationColombiaCOLMiddle and South America
67770Cassava fermenterColombiaCOLMiddle and South America
120776Cassava fermentation processColombiaCOLMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Vegetable (incl. Grains)
#Engineered#Food production#Food
#Host#Plants#Shrub (Scrub)
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_66110.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_2517;97_3711;98_46648;99_66110&stattab=map
  • Last taxonomy: Lacticaseibacillus
  • 16S sequence: LC383830
  • Sequence Identity:
  • Total samples: 3577
  • soil counts: 244
  • aquatic counts: 655
  • animal counts: 2488
  • plant counts: 190

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50051Risk group (German classification)
1207761Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5005Lactobacillus manihotivorans strain OND 32 16S ribosomal RNA gene, complete sequenceAF0001621559ena88233
67770Lactobacillus manihotivorans JCM 12514 gene for 16S ribosomal RNA, partial sequenceLC3838301506ena88233

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus manihotivorans DSM 13343 = JCM 125141423769.4wgspatric1423769
66792Lactobacillus manihotivorans DSM 13343 = JCM 125141423769.3wgspatric1423769
66792Lacticaseibacillus manihotivorans JCM 125142728369672draftimg1423769
66792Lacticaseibacillus manihotivorans DSM 133432713896728draftimg1423769
67770Lacticaseibacillus manihotivorans DSM 13343 = JCM 12514GCA_001435035scaffoldncbi1423769
67770Lacticaseibacillus manihotivorans DSM 13343 = JCM 12514GCA_001311155contigncbi1423769

GC content

@refGC-contentmethod
500548.4high performance liquid chromatography (HPLC)
6777047.7genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.344no
gram-positiveyes94.422no
anaerobicno90.419no
halophileyes83.991no
spore-formingno92.02no
thermophileno99.535yes
glucose-utilyes91.411no
aerobicno97.137no
flagellatedno96.514no
glucose-fermentyes86.065no

External links

@ref: 5005

culture collection no.: CCUG 42894, DSM 13343, LMG 18010, OND 32, JCM 12514, CIP 105851, KCTC 21010

straininfo link

  • @ref: 76057
  • straininfo: 14473

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9828412Lactobacillus manihotivorans sp. nov., a new starch-hydrolysing lactic acid bacterium isolated during cassava sour starch fermentation.Morlon-Guyot J, Guyot JP, Pot B, Jacobe de Haut I, Raimbault MInt J Syst Bacteriol10.1099/00207713-48-4-11011998Base Composition, DNA Fingerprinting, DNA, Bacterial/chemistry, Electrophoresis, Polyacrylamide Gel, Fermentation, Food Microbiology, Genes, rRNA, Lactic Acid/metabolism, Lactobacillus/*classification/growth & development/isolation & purification/metabolism, Manihot/*metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Starch/*metabolism, Terminology as TopicMetabolism
Metabolism10735257Effect of pH control on lactic acid fermentation of starch by Lactobacillus manihotivorans LMG 18010T.Guyot JP, Calderon M, Morlon-Guyot JJ Appl Microbiol10.1046/j.1365-2672.2000.00953.x2000Biomass, Bioreactors, Fermentation, Glucose/metabolism, Hydrogen-Ion Concentration, Lactic Acid/*metabolism, Lactobacillus/growth & development/*metabolism, Starch/*metabolism, alpha-Amylases/metabolism
Enzymology10938420Purification and characterization of an extracellular alpha-amylase produced by Lactobacillus manihotivorans LMG 18010(T), an amylolytic lactic acid bacterium.Aguilar G, Morlon-Guyot J, Trejo-Aguilar B, Guyot JPEnzyme Microb Technol10.1016/s0141-0229(00)00230-12000
Metabolism11518431Effect of different cultivation conditions on Lactobacillus manihotivorans OND32T, an amylolytic lactobacillus isolated from sour starch cassava fermentation.Guyot JP, Morlon-Guyot JInt J Food Microbiol10.1016/s0168-1605(01)00444-52001Amylases/*metabolism, Carbohydrate Metabolism, Carbon Dioxide/metabolism, Culture Media, Fermentation, Lactic Acid/metabolism, Lactobacillus/enzymology/growth & development/*metabolism, Manihot/metabolism/*microbiology, Nitrogen/metabolism, Oxygen/metabolism, Starch/metabolismCultivation
Phylogeny12361282Lactobacillus pantheris sp. nov., isolated from faeces of a jaguar.Liu B, Dong XInt J Syst Evol Microbiol10.1099/00207713-52-5-17452002Animals, Animals, Zoo/microbiology, Base Composition, Carnivora/microbiology, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Feces/microbiology, Fermentation, Lactobacillus/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicGenetics
Phylogeny12927722Characterization and differentiation of Lactobacillus manihotivorans strains isolated from cassava sour starch.Guyot JP, Brizuela MA, Rodriguez Sanoja R, Morlon-Guyot JInt J Food Microbiol10.1016/s0168-1605(03)00048-52003Fermentation, *Food Microbiology, Hydrogen-Ion Concentration, Lactic Acid/metabolism, Lactobacillus/*classification/isolation & purification, Manihot/*microbiology, Phylogeny, Starch/*chemistry/metabolism, TemperatureBiotechnology
Phylogeny21724957Lactobacillus nasuensis sp. nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus.Cai Y, Pang H, Kitahara M, Ohkuma MInt J Syst Evol Microbiol10.1099/ijs.0.031781-02011Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gases/metabolism, Glucose/metabolism, Lactic Acid/*metabolism, Lactobacillus/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Silage/*microbiologyMetabolism
Phylogeny33724174Lacticaseibacillus absianus sp. nov., isolated from the cecum of a mini-pig.Bai L, Paek J, Shin Y, Park HY, Chang YHInt J Syst Evol Microbiol10.1099/ijsem.0.0047522021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5005Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13343)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13343
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34638Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17916
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76057Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14473.1StrainInfo: A central database for resolving microbial strain identifiers
120776Curators of the CIPCollection of Institut Pasteur (CIP 105851)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105851