Strain identifier
BacDive ID: 6582
Type strain:
Species: Paralactobacillus selangorensis
Strain history: CIP <- 2000, LMG <- G. Rusul, DFS, Univ. Pertanian, Malaysia <- J. Leisner, DVM, Univ. Frederiksberg, Denmark
NCBI tax ID(s): 81857 (species)
General
@ref: 5006
BacDive-ID: 6582
DSM-Number: 13344
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Paralactobacillus selangorensis DSM 13344 is an anaerobe, mesophilic bacterium that was isolated from "chili bo".
NCBI tax id
- NCBI tax id: 81857
- Matching level: species
strain history
@ref | history |
---|---|
5006 | <- LMG <- G. Rusul <- J. Leisner |
67770 | CCUG 43347 <-- LMG 17710 <-- G. Rusul <-- J. J. Leisner 9-22. |
122552 | CIP <- 2000, LMG <- G. Rusul, DFS, Univ. Pertanian, Malaysia <- J. Leisner, DVM, Univ. Frederiksberg, Denmark |
doi: 10.13145/bacdive6582.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Paralactobacillus
- species: Paralactobacillus selangorensis
- full scientific name: Paralactobacillus selangorensis Leisner et al. 2000
synonyms
@ref synonym 20215 Paralactobacillus selangorensis 20215 Lactobacillus selangorensis
@ref: 5006
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Paralactobacillus
species: Paralactobacillus selangorensis
full scientific name: Paralactobacillus selangorensis Leisner et al. 2000
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 96.572 | ||
69480 | 100 | positive | ||
122552 | no | positive | rod-shaped |
colony morphology
- @ref: 55805
- incubation period: 2-3 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5006 | MRS MEDIUM (DSMZ Medium 11) | yes | https://mediadive.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
38630 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
122552 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5006 | positive | growth | 30 | mesophilic |
38630 | positive | growth | 30 | mesophilic |
55805 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
122552 | positive | growth | 15-37 | |
122552 | no | growth | 10 | psychrophilic |
122552 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
55805 | anaerobe |
122552 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.99 |
murein
- @ref: 5006
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
122552 | nitrate | - | reduction | 17632 |
122552 | nitrite | - | reduction | 16301 |
122552 | nitrate | + | respiration | 17632 |
antibiotic resistance
- @ref: 122552
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite tests
- @ref: 122552
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
122552 | oxidase | - | |
122552 | alcohol dehydrogenase | - | 1.1.1.1 |
122552 | catalase | - | 1.11.1.6 |
122552 | lysine decarboxylase | - | 4.1.1.18 |
122552 | ornithine decarboxylase | - | 4.1.1.17 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122552 | - | - | - | - | - | + | + | + | - | - | + | + | - | - | - | + | + | + | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122552 | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | +/- | +/- | +/- | +/- | - | - | - | +/- | - | - | - | - | - | - | +/- | - | - | +/- | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
5006 | "chili bo" | Malaysia | MYS | Asia | |
55805 | Chili bo | Malaysia | MYS | Asia | |
67770 | Chili bo, a Malaysian food ingredient | Malaysia | MYS | Asia | Pataling Jaya, Selangor DE |
122552 | Chili bo | Malaysia | MYS | Asia |
isolation source categories
- Cat1: #Engineered
- Cat2: #Food production
- Cat3: #Food
taxonmaps
- @ref: 69479
- File name: preview.99_8048.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_3719;97_4571;98_5866;99_8048&stattab=map
- Last taxonomy: Lactobacillus selangorensis subclade
- 16S sequence: LC589221
- Sequence Identity:
- Total samples: 2613
- soil counts: 252
- aquatic counts: 166
- animal counts: 2101
- plant counts: 94
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5006 | 1 | Risk group (German classification) |
122552 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
5006 | Paralactobacillus selangorensis strain LMG 17710 16S ribosomal RNA (rrs) gene, partial sequence | AF049745 | 1495 | ena | 81857 |
67770 | Lactobacillus selangorensis JCM 19618 gene for 16S rRNA, partial sequence | LC589221 | 1494 | ena | 81857 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Lactobacillus selangorensis strain DSM 13344 | 81857.4 | wgs | patric | 81857 |
66792 | Lactobacillus selangorensis DSM 13344 | 2663763515 | draft | img | 81857 |
67770 | Lactobacillus selangorensis ATCC BAA-66 | GCA_001436805 | scaffold | ncbi | 81857 |
67770 | Lactobacillus selangorensis DSM 13344 | GCA_001437205 | contig | ncbi | 81857 |
GC content
@ref | GC-content | method |
---|---|---|
5006 | 46 | high performance liquid chromatography (HPLC) |
67770 | 46.1 | high performance liquid chromatography (HPLC) |
67770 | 46 | genome sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 93.227 | no |
flagellated | no | 98.152 | no |
gram-positive | yes | 95.905 | no |
anaerobic | no | 74.252 | yes |
halophile | yes | 81.404 | no |
spore-forming | no | 97.548 | no |
thermophile | no | 99.351 | yes |
glucose-util | yes | 92.145 | no |
aerobic | no | 97.482 | yes |
glucose-ferment | yes | 89.29 | no |
External links
@ref: 5006
culture collection no.: DSM 13344, ATCC BAA 66, CCUG 43347, CIP 106482, LMG 17710, JCM 19618, NCIMB 13672
straininfo link
- @ref: 76056
- straininfo: 10757
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 10826783 | Description of Paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a Malaysian food ingredient. | Leisner JJ, Vancanneyt M, Goris J, Christensen H, Rusul G | Int J Syst Evol Microbiol | 10.1099/00207713-50-1-19 | 2000 | Acetic Acid/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, Ethanol/metabolism, Fatty Acids/analysis, *Food Microbiology, Lactic Acid/metabolism, Lactobacillaceae/*classification/genetics/*isolation & purification/physiology, Malaysia, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics | Metabolism |
Phylogeny | 21296926 | Reclassification of Paralactobacillus selangorensis Leisner et al. 2000 as Lactobacillus selangorensis comb. nov. | Haakensen M, Pittet V, Ziola B | Int J Syst Evol Microbiol | 10.1099/ijs.0.027755-0 | 2011 | Bacterial Proteins/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Lactobacillaceae/*classification/genetics/isolation & purification, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics | Enzymology |
Phylogeny | 23002044 | Lactobacillus shenzhenensis sp. nov., isolated from a fermented dairy beverage. | Zou Y, Liu F, Fang C, Wan D, Yang R, Su Q, Yang R, Zhao J | Int J Syst Evol Microbiol | 10.1099/ijs.0.041111-0 | 2012 | Bacterial Typing Techniques, Base Composition, Beverages/*microbiology, China, DNA, Bacterial/genetics, Dairy Products/*microbiology, Fatty Acids/analysis, *Fermentation, Food Microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5006 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13344) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13344 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
38630 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18617 | ||||
55805 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 43347) | https://www.ccug.se/strain?id=43347 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
76056 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10757.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
122552 | Curators of the CIP | Collection of Institut Pasteur (CIP 106482) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106482 |