Strain identifier

BacDive ID: 6582

Type strain: Yes

Species: Paralactobacillus selangorensis

Strain history: CIP <- 2000, LMG <- G. Rusul, DFS, Univ. Pertanian, Malaysia <- J. Leisner, DVM, Univ. Frederiksberg, Denmark

NCBI tax ID(s): 81857 (species)

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General

@ref: 5006

BacDive-ID: 6582

DSM-Number: 13344

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Paralactobacillus selangorensis DSM 13344 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from "chili bo".

NCBI tax id

  • NCBI tax id: 81857
  • Matching level: species

strain history

@refhistory
5006<- LMG <- G. Rusul <- J. Leisner
67770CCUG 43347 <-- LMG 17710 <-- G. Rusul <-- J. J. Leisner 9-22.
122552CIP <- 2000, LMG <- G. Rusul, DFS, Univ. Pertanian, Malaysia <- J. Leisner, DVM, Univ. Frederiksberg, Denmark

doi: 10.13145/bacdive6582.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Paralactobacillus
  • species: Paralactobacillus selangorensis
  • full scientific name: Paralactobacillus selangorensis Leisner et al. 2000

  • synonyms

    @refsynonym
    20215Paralactobacillus selangorensis
    20215Lactobacillus selangorensis

@ref: 5006

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Paralactobacillus

species: Paralactobacillus selangorensis

full scientific name: Paralactobacillus selangorensis Leisner et al. 2000

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
122552positiverod-shapedno
125438positive92.798

colony morphology

  • @ref: 55805
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5006MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38630MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122552CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
5006positivegrowth30
38630positivegrowth30
55805positivegrowth30
67770positivegrowth30
122552positivegrowth15-37
122552nogrowth10
122552nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
55805anaerobe
122552facultative anaerobe
125439microaerophile95.6

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 98.9

murein

  • @ref: 5006
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12255217632nitrate-reduction
12255216301nitrite-reduction
12255217632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

antibiotic resistance

  • @ref: 122552
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 122552
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase+3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
122552oxidase-
122552alcohol dehydrogenase-1.1.1.1
122552catalase-1.11.1.6
122552lysine decarboxylase-4.1.1.18
122552ornithine decarboxylase-4.1.1.17
68382alpha-fucosidase-3.2.1.51
68382trypsin-3.4.21.4

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122552-----+++--++---+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122552----------+++-----------+/-+/-+/-+/----+/-------+/---+/--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5006"chili bo"MalaysiaMYSAsia
55805Chili boMalaysiaMYSAsia
67770Chili bo, a Malaysian food ingredientMalaysiaMYSAsiaPataling Jaya, Selangor DE
122552Chili boMalaysiaMYSAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Food

taxonmaps

  • @ref: 69479
  • File name: preview.99_8048.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_3719;97_4571;98_5866;99_8048&stattab=map
  • Last taxonomy: Lactobacillus selangorensis subclade
  • 16S sequence: LC589221
  • Sequence Identity:
  • Total samples: 2613
  • soil counts: 252
  • aquatic counts: 166
  • animal counts: 2101
  • plant counts: 94

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50061Risk group (German classification)
1225521Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5006Paralactobacillus selangorensis strain LMG 17710 16S ribosomal RNA (rrs) gene, partial sequenceAF0497451495nuccore81857
67770Lactobacillus selangorensis JCM 19618 gene for 16S rRNA, partial sequenceLC5892211494nuccore81857

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus selangorensis strain DSM 1334481857.4wgspatric81857
66792Lactobacillus selangorensis DSM 133442663763515draftimg81857
67770Lactobacillus selangorensis ATCC BAA-66GCA_001436805scaffoldncbi81857
67770Lactobacillus selangorensis DSM 13344GCA_001437205contigncbi81857

GC content

@refGC-contentmethod
500646high performance liquid chromatography (HPLC)
6777046.1high performance liquid chromatography (HPLC)
6777046genome sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes92.798no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no81.932no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no94.477yes
125438spore-formingspore-formingAbility to form endo- or exosporesno86.07no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno95no
125438motile2+flagellatedAbility to perform flagellated movementno89.5no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.9
125439BacteriaNetmotilityAbility to perform movementno70.1
125439BacteriaNetgram_stainReaction to gram-stainingpositive65.9
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthmicroaerophile95.6

External links

@ref: 5006

culture collection no.: DSM 13344, ATCC BAA-66, CCUG 43347, CIP 106482, LMG 17710, JCM 19618, NCIMB 13672

straininfo link

  • @ref: 76056
  • straininfo: 10757

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10826783Description of Paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a Malaysian food ingredient.Leisner JJ, Vancanneyt M, Goris J, Christensen H, Rusul GInt J Syst Evol Microbiol10.1099/00207713-50-1-192000Acetic Acid/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, Ethanol/metabolism, Fatty Acids/analysis, *Food Microbiology, Lactic Acid/metabolism, Lactobacillaceae/*classification/genetics/*isolation & purification/physiology, Malaysia, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny21296926Reclassification of Paralactobacillus selangorensis Leisner et al. 2000 as Lactobacillus selangorensis comb. nov.Haakensen M, Pittet V, Ziola BInt J Syst Evol Microbiol10.1099/ijs.0.027755-02011Bacterial Proteins/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Lactobacillaceae/*classification/genetics/isolation & purification, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsEnzymology
Phylogeny23002044Lactobacillus shenzhenensis sp. nov., isolated from a fermented dairy beverage.Zou Y, Liu F, Fang C, Wan D, Yang R, Su Q, Yang R, Zhao JInt J Syst Evol Microbiol10.1099/ijs.0.041111-02012Bacterial Typing Techniques, Base Composition, Beverages/*microbiology, China, DNA, Bacterial/genetics, Dairy Products/*microbiology, Fatty Acids/analysis, *Fermentation, Food Microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5006Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13344)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13344
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38630Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18617
55805Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43347)https://www.ccug.se/strain?id=43347
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76056Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10757.1StrainInfo: A central database for resolving microbial strain identifiers
122552Curators of the CIPCollection of Institut Pasteur (CIP 106482)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106482
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1