Strain identifier

BacDive ID: 6579

Type strain: Yes

Species: Companilactobacillus bobalius

Strain Designation: 203, Lb 203

Strain history: CIP <- 2008, CECT, Lactobacillus bobalius <- R. Manes <- A.M. Rodas, Valencia Univ., Valencia, Spain: strain 203

NCBI tax ID(s): 1423788 (strain), 2801451 (species)

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General

@ref: 8248

BacDive-ID: 6579

DSM-Number: 19674

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Companilactobacillus bobalius 203 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Spanish Bobal grape must.

NCBI tax id

NCBI tax idMatching level
2801451species
1423788strain

strain history

@refhistory
8248<- R. Manes Lazaro, Enolab, Valencia, Spain; strain 203
67770DSM 19674 <-- R. Mañes-Lázaro 203.
121566CIP <- 2008, CECT, Lactobacillus bobalius <- R. Manes <- A.M. Rodas, Valencia Univ., Valencia, Spain: strain 203

doi: 10.13145/bacdive6579.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Companilactobacillus
  • species: Companilactobacillus bobalius
  • full scientific name: Companilactobacillus bobalius (Mañes-Lázaro et al. 2008) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus bobalius

@ref: 8248

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Companilactobacillus

species: Companilactobacillus bobalius

full scientific name: Companilactobacillus bobalius (Mañes-Lázaro et al. 2008) Zheng et al. 2020

strain designation: 203, Lb 203

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32562positive1.65-3.41 µm0.71-1.03 µmrod-shapedno
69480no95.879
69480positive100
121566positiverod-shapedno

pigmentation

  • @ref: 32562
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8248MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33686MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121566CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8248positivegrowth28mesophilic
32562positivegrowth15-45
32562positiveoptimum15-45
33686positivegrowth37mesophilic
67770positivegrowth28mesophilic
121566positivegrowth25-37mesophilic
121566nogrowth15psychrophilic
121566nogrowth45thermophilic

culture pH

@refabilitytypepHPH range
32562positivegrowth3.3-8acidophile
32562positiveoptimum3.3-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8248microaerophile
32562anaerobe
121566facultative anaerobe

spore formation

@refspore formationconfidence
32562no
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentration
32562NaClpositivegrowth5 %
32562NaClpositiveoptimum5 %

murein

  • @ref: 8248
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 32562
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3256216947citrate+carbon source
3256225115malate+carbon source
325624853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12156617632nitrate-reduction
12156616301nitrite-reduction
12156617632nitrate+respiration

metabolite production

  • @ref: 121566
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 121566
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
121566oxidase-
121566alcohol dehydrogenase-1.1.1.1
121566catalase-1.11.1.6
121566lysine decarboxylase-4.1.1.18
121566ornithine decarboxylase-4.1.1.17
121566urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121566-+--------+++--+++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8248-----+-----+++--------+++++++--++-+----+--------+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8248Spanish Bobal grape mustCuevas de UtielSpainESPEurope
67770Spanish Bobal grape must
121566Bobal grape mustSpainESPEurope1997

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Beverage
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2939.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_954;97_1113;98_2270;99_2939&stattab=map
  • Last taxonomy: Companilactobacillus paralimentarius
  • 16S sequence: AY681134
  • Sequence Identity:
  • Total samples: 738
  • soil counts: 72
  • aquatic counts: 52
  • animal counts: 569
  • plant counts: 45

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
82481Risk group (German classification)
1215661Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8248
  • description: Lactobacillus paralimentarius strain 203 16S ribosomal RNA gene, complete sequence
  • accession: AY681134
  • length: 1527
  • database: ena
  • NCBI tax ID: 83526

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Companilactobacillus bobalius 203GCA_009764355contigncbi2801451
66792Lactobacillus paralimentarius DSM 196741423788.3wgspatric1423788
66792Lactobacillus paralimentarius strain KACC 1634383526.4wgspatric83526
66792Lactobacillus paralimentarius strain NBRC 10714983526.11wgspatric83526
66792Lactobacillus paralimentarius DSM 196742695420442draftimg1423788
67770Companilactobacillus bobalius DSM 19674GCA_001434855scaffoldncbi1423788
67770Companilactobacillus paralimentarius NBRC 107149GCA_007991895contigncbi83526
67770Companilactobacillus bobalius KACC 16343GCA_002179915contigncbi2801451

GC content

@refGC-contentmethod
824836.8high performance liquid chromatography (HPLC)
824834
6777035.3genome sequence analysis
6777034.03high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.57yes
gram-positiveyes94.581no
anaerobicno85.427no
halophileyes90.815no
spore-formingno94.01yes
glucose-utilyes91.161no
aerobicno96.367yes
flagellatedno98.209yes
glucose-fermentyes89.886no
thermophileno99.699yes

External links

@ref: 8248

culture collection no.: DSM 19674, CECT 7310, JCM 16180, CIP 109926, KACC 16343, LMG 25239, NBRC 107149

straininfo link

  • @ref: 76053
  • straininfo: 360273

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19060043Lactobacillus bobalius sp. nov., a lactic acid bacterium isolated from Spanish Bobal grape must.Manes-Lazaro R, Ferrer S, Rodas AM, Urdiain M, Pardo IInt J Syst Evol Microbiol10.1099/ijs.0.65695-02008DNA, Ribosomal Spacer/genetics, Lactobacillus/chemistry/*classification/genetics/*physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Species Specificity, Vitis/*microbiologyGenetics
Phylogeny22140166Reclassification of Lactobacillus kimchii and Lactobacillus bobalius as later subjective synonyms of Lactobacillus paralimentarius.Pang H, Kitahara M, Tan Z, Wang Y, Qin G, Ohkuma M, Cai YInt J Syst Evol Microbiol10.1099/ijs.0.035329-02011DNA, Bacterial/genetics, Fermentation, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Silage/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
8248Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19674)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19674
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32562Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877828776041
33686Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7725
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76053Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID360273.1StrainInfo: A central database for resolving microbial strain identifiers
121566Curators of the CIPCollection of Institut Pasteur (CIP 109926)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109926