Strain identifier

BacDive ID: 6574

Type strain: No

Species: Schleiferilactobacillus perolens

Strain Designation: L 533, L533

Strain history: CIP <- 2006, DSMZ <- I. Bohak <- W. back: strain L533

NCBI tax ID(s): 100468 (species)

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General

@ref: 4799

BacDive-ID: 6574

DSM-Number: 12745

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Schleiferilactobacillus perolens L 533 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from orange lemonade.

NCBI tax id

  • NCBI tax id: 100468
  • Matching level: species

strain history

@refhistory
4799<- I. Bohak <- W. Back; L 533
119840CIP <- 2006, DSMZ <- I. Bohak <- W. back: strain L533

doi: 10.13145/bacdive6574.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Schleiferilactobacillus
  • species: Schleiferilactobacillus perolens
  • full scientific name: Schleiferilactobacillus perolens (Back et al. 2000) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus perolens

@ref: 4799

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Schleiferilactobacillus

species: Schleiferilactobacillus perolens

full scientific name: Schleiferilactobacillus perolens (Back et al. 2000) Zheng et al. 2020

strain designation: L 533, L533

type strain: no

Morphology

cell morphology

  • @ref: 119840
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4799MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
36786MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119840CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
4799positivegrowth28mesophilic
36786positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119840
  • oxygen tolerance: anaerobe

murein

  • @ref: 4799
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-lyxose-builds acid from62318
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose+builds acid from16634
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119840nitrate-reduction17632
119840nitrite-reduction16301

metabolite production

  • @ref: 119840
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119840oxidase-
119840alcohol dehydrogenase-1.1.1.1
119840catalase-1.11.1.6
119840lysine decarboxylase-4.1.1.18
119840ornithine decarboxylase-4.1.1.17
119840urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119840-+------+-----+-+---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
4799---++-----++++-----+-++++++++-+++--+------+-------
119840--+/-+-----++++-----+/--+/-++++++++/-+/-++-+/-++/---++/--+/--+/---++/--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4799orange lemonadeNetherlandsNLDEurope
119840Orange lemonadeNetherlandsNLDEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Beverage

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
47991Risk group (German classification)
1198401Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Lactobacillus perolens 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA integenic spacer gene, complete sequence; and 23S ribosomal RNA gene, partial sequence
  • accession: DQ314275
  • length: 411
  • database: ena
  • NCBI tax ID: 100468

External links

@ref: 4799

culture collection no.: DSM 12745, CIP 109156, LMG 18938

straininfo link

  • @ref: 76048
  • straininfo: 13213

literature

  • topic: Metabolism
  • Pubmed-ID: 16261858
  • title: Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables 'Suan cai' in Harbin, Northeastern China and Lactobacillus perolens DSM 12745.
  • authors: Miyamoto M, Seto Y, Hao DH, Teshima T, Sun YB, Kabuki T, Yao LB, Nakajima H
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2005.04.001
  • year: 2005
  • mesh: Acetates/metabolism, Arabinose/metabolism, Base Composition, China, DNA Probes, DNA, Bacterial/genetics, Ethanol/metabolism, Fermentation, *Food Microbiology, Gluconates/metabolism, Glucose/metabolism, Lactic Acid/biosynthesis, Lactobacillus/*classification/genetics/metabolism, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Vegetables/metabolism/*microbiology
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4799Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12745)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12745
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36786Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6866
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
76048Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13213.1StrainInfo: A central database for resolving microbial strain identifiers
119840Curators of the CIPCollection of Institut Pasteur (CIP 109156)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109156