Strain identifier
BacDive ID: 6571
Type strain:
Species: Lactobacillus amylolyticus
Strain Designation: LA 5
Strain history: CIP <- 2002, CCUG <- 1998, DSMZ <- I. Bohak: strain LA 5
NCBI tax ID(s): 585524 (strain), 83683 (species)
General
@ref: 4433
BacDive-ID: 6571
DSM-Number: 11664
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive, rod-shaped
description: Lactobacillus amylolyticus LA 5 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from acidified beer wort.
NCBI tax id
NCBI tax id | Matching level |
---|---|
83683 | species |
585524 | strain |
strain history
@ref | history |
---|---|
4433 | <- I. Bohak, LA 5 |
67770 | LMG 18796 <-- I. Bohak LA 5 <-- M. Leibhard. |
116356 | CIP <- 2002, CCUG <- 1998, DSMZ <- I. Bohak: strain LA 5 |
doi: 10.13145/bacdive6571.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Lactobacillus
- species: Lactobacillus amylolyticus
- full scientific name: Lactobacillus amylolyticus Bohak et al. 1999
@ref: 4433
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Lactobacillus
species: Lactobacillus amylolyticus
full scientific name: Lactobacillus amylolyticus Bohak et al. 1999
strain designation: LA 5
type strain: yes
Morphology
cell morphology
- @ref: 116356
- gram stain: positive
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4433 | PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) | yes | https://mediadive.dsmz.de/medium/231 | Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
40470 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
116356 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4433 | positive | growth | 45 | thermophilic |
40470 | positive | growth | 45 | thermophilic |
54985 | positive | growth | 37-45 | |
67770 | positive | growth | 37 | mesophilic |
116356 | positive | growth | 30-45 | |
116356 | no | growth | 10 | psychrophilic |
116356 | no | growth | 15 | psychrophilic |
116356 | no | growth | 22 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 54985
- oxygen tolerance: anaerobe
murein
- @ref: 4433
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
116356 | nitrate | - | reduction | 17632 |
116356 | nitrite | - | reduction | 16301 |
antibiotic resistance
- @ref: 116356
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite tests
- @ref: 116356
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
116356 | oxidase | - | |
116356 | alcohol dehydrogenase | + | 1.1.1.1 |
116356 | catalase | - | 1.11.1.6 |
116356 | lysine decarboxylase | - | 4.1.1.18 |
116356 | ornithine decarboxylase | - | 4.1.1.17 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116356 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | + | - | - | - | - |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
4433 | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
4433 | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
4433 | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
116356 | - | - | - | - | - | - | - | - | - | +/- | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
4433 | acidified beer wort | Germany | DEU | Europe |
54985 | Acidified beer wort | |||
67770 | Brewery | |||
116356 | Acidified beer wort |
isolation source categories
Cat1 | Cat2 |
---|---|
#Engineered | #Food production |
#Condition | #Acidic |
taxonmaps
- @ref: 69479
- File name: preview.99_5535.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_1120;97_2520;98_4130;99_5535&stattab=map
- Last taxonomy: Lactobacillus
- 16S sequence: LC383824
- Sequence Identity:
- Total samples: 2742
- soil counts: 39
- aquatic counts: 265
- animal counts: 2338
- plant counts: 100
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4433 | 1 | Risk group (German classification) |
116356 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
4433 | Lactobacillus amylolyticus 16S rRNA gene | Y17361 | 1558 | ena | 585524 |
67770 | Lactobacillus amylolyticus partial 16S rRNA gene, type strain CCUG 39901T | FR683095 | 1443 | ena | 83683 |
67770 | Lactobacillus amylolyticus JCM 12529 gene for 16S ribosomal RNA, partial sequence | LC383824 | 1502 | ena | 83683 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Lactobacillus amylolyticus DSM 11664 | 585524.3 | wgs | patric | 585524 |
66792 | Lactobacillus amylolyticus DSM 11664 | 585524.9 | wgs | patric | 585524 |
66792 | Lactobacillus amylolyticus strain DSM 11664 | 83683.14 | wgs | patric | 83683 |
66792 | Lactobacillus amylolyticus DSM 11664 | 2671180773 | draft | img | 585524 |
66792 | Lactobacillus amylolyticus DSM 11664 | 647000263 | draft | img | 585524 |
67770 | Lactobacillus amylolyticus DSM 11664 | GCA_000178475 | contig | ncbi | 585524 |
67770 | Lactobacillus amylolyticus DSM 11664 | GCA_001435665 | contig | ncbi | 585524 |
67770 | Lactobacillus amylolyticus DSM 11664 | GCA_004354545 | contig | ncbi | 83683 |
GC content
@ref | GC-content | method |
---|---|---|
4433 | 39 | thermal denaturation, midpoint method (Tm) |
67770 | 38.2 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
gram-positive | yes | 95.316 | no |
anaerobic | yes | 87.381 | yes |
halophile | yes | 84.622 | no |
spore-forming | no | 96.704 | no |
glucose-util | yes | 91.465 | no |
thermophile | no | 97.96 | no |
flagellated | no | 98.429 | no |
aerobic | no | 97.254 | yes |
motile | no | 96.72 | no |
glucose-ferment | yes | 87.753 | no |
External links
@ref: 4433
culture collection no.: DSM 11664, CCUG 39901, CIP 107338, JCM 12529, LMG 18796
straininfo link
- @ref: 76045
- straininfo: 12437
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 9779604 | Lactobacillus amylolyticus sp. nov., isolated from beer malt and beer wort. | Bohak I, Back W, Richter L, Ehrmann M, Ludwig W, Schleifer KH | Syst Appl Microbiol | 10.1016/S0723-2020(98)80045-3 | 1998 | Bacterial Typing Techniques, Beer/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Humans, Lactobacillus/*classification/genetics/*isolation & purification, Nucleic Acid Hybridization, Oligonucleotide Probes, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA | Pathogenicity |
Phylogeny | 31560297 | Lactobacillus xujianguonis sp. nov., isolated from faeces of Marmota himalayana. | Meng J, Jin D, Yang J, Lai XH, Pu J, Zhu W, Huang Y, Liang H, Lu S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003598 | 2020 | Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Genes, Bacterial, Lactobacillus/*classification/isolation & purification, Marmota/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tibet | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4433 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11664) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11664 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
40470 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4763 | ||||
54985 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 39901) | https://www.ccug.se/strain?id=39901 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76045 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID12437.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116356 | Curators of the CIP | Collection of Institut Pasteur (CIP 107338) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107338 |