Strain identifier

BacDive ID: 6571

Type strain: Yes

Species: Lactobacillus amylolyticus

Strain Designation: LA 5

Strain history: CIP <- 2002, CCUG <- 1998, DSMZ <- I. Bohak: strain LA 5

NCBI tax ID(s): 585524 (strain), 83683 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4433

BacDive-ID: 6571

DSM-Number: 11664

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive, rod-shaped

description: Lactobacillus amylolyticus LA 5 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from acidified beer wort.

NCBI tax id

NCBI tax idMatching level
83683species
585524strain

strain history

@refhistory
4433<- I. Bohak, LA 5
67770LMG 18796 <-- I. Bohak LA 5 <-- M. Leibhard.
116356CIP <- 2002, CCUG <- 1998, DSMZ <- I. Bohak: strain LA 5

doi: 10.13145/bacdive6571.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus amylolyticus
  • full scientific name: Lactobacillus amylolyticus Bohak et al. 1999

@ref: 4433

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus amylolyticus

full scientific name: Lactobacillus amylolyticus Bohak et al. 1999

strain designation: LA 5

type strain: yes

Morphology

cell morphology

  • @ref: 116356
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4433PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)yeshttps://mediadive.dsmz.de/medium/231Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40470MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
116356CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
4433positivegrowth45thermophilic
40470positivegrowth45thermophilic
54985positivegrowth37-45
67770positivegrowth37mesophilic
116356positivegrowth30-45
116356nogrowth10psychrophilic
116356nogrowth15psychrophilic
116356nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 54985
  • oxygen tolerance: anaerobe

murein

  • @ref: 4433
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116356nitrate-reduction17632
116356nitrite-reduction16301

antibiotic resistance

  • @ref: 116356
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 116356
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
116356oxidase-
116356alcohol dehydrogenase+1.1.1.1
116356catalase-1.11.1.6
116356lysine decarboxylase-4.1.1.18
116356ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116356----------++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
4433-----------+----------------+--+------------------
4433-----+-------------------+-----+------------------
4433-----------+++-----------+--+--+------------------
116356---------+/-+++--------------+--+------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4433acidified beer wortGermanyDEUEurope
54985Acidified beer wort
67770Brewery
116356Acidified beer wort

isolation source categories

Cat1Cat2
#Engineered#Food production
#Condition#Acidic

taxonmaps

  • @ref: 69479
  • File name: preview.99_5535.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_1120;97_2520;98_4130;99_5535&stattab=map
  • Last taxonomy: Lactobacillus
  • 16S sequence: LC383824
  • Sequence Identity:
  • Total samples: 2742
  • soil counts: 39
  • aquatic counts: 265
  • animal counts: 2338
  • plant counts: 100

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
44331Risk group (German classification)
1163561Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4433Lactobacillus amylolyticus 16S rRNA geneY173611558ena585524
67770Lactobacillus amylolyticus partial 16S rRNA gene, type strain CCUG 39901TFR6830951443ena83683
67770Lactobacillus amylolyticus JCM 12529 gene for 16S ribosomal RNA, partial sequenceLC3838241502ena83683

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus amylolyticus DSM 11664585524.3wgspatric585524
66792Lactobacillus amylolyticus DSM 11664585524.9wgspatric585524
66792Lactobacillus amylolyticus strain DSM 1166483683.14wgspatric83683
66792Lactobacillus amylolyticus DSM 116642671180773draftimg585524
66792Lactobacillus amylolyticus DSM 11664647000263draftimg585524
67770Lactobacillus amylolyticus DSM 11664GCA_000178475contigncbi585524
67770Lactobacillus amylolyticus DSM 11664GCA_001435665contigncbi585524
67770Lactobacillus amylolyticus DSM 11664GCA_004354545contigncbi83683

GC content

@refGC-contentmethod
443339thermal denaturation, midpoint method (Tm)
6777038.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes95.316no
anaerobicyes87.381yes
halophileyes84.622no
spore-formingno96.704no
glucose-utilyes91.465no
thermophileno97.96no
flagellatedno98.429no
aerobicno97.254yes
motileno96.72no
glucose-fermentyes87.753no

External links

@ref: 4433

culture collection no.: DSM 11664, CCUG 39901, CIP 107338, JCM 12529, LMG 18796

straininfo link

  • @ref: 76045
  • straininfo: 12437

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9779604Lactobacillus amylolyticus sp. nov., isolated from beer malt and beer wort.Bohak I, Back W, Richter L, Ehrmann M, Ludwig W, Schleifer KHSyst Appl Microbiol10.1016/S0723-2020(98)80045-31998Bacterial Typing Techniques, Beer/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Humans, Lactobacillus/*classification/genetics/*isolation & purification, Nucleic Acid Hybridization, Oligonucleotide Probes, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNAPathogenicity
Phylogeny31560297Lactobacillus xujianguonis sp. nov., isolated from faeces of Marmota himalayana.Meng J, Jin D, Yang J, Lai XH, Pu J, Zhu W, Huang Y, Liang H, Lu SInt J Syst Evol Microbiol10.1099/ijsem.0.0035982020Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Genes, Bacterial, Lactobacillus/*classification/isolation & purification, Marmota/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4433Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11664)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11664
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40470Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4763
54985Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39901)https://www.ccug.se/strain?id=39901
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76045Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12437.1StrainInfo: A central database for resolving microbial strain identifiers
116356Curators of the CIPCollection of Institut Pasteur (CIP 107338)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107338