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Strain identifier

BacDive ID: 6567

Type strain: Yes

Species: Lentilactobacillus parakefiri

Strain history: S. Takizawa GCL 1731.

NCBI tax ID(s): 1423787 (strain), 152332 (species)

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General

@ref: 3924

BacDive-ID: 6567

DSM-Number: 10551

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Lentilactobacillus parakefiri CCUG 39468 is an anaerobe, mesophilic bacterium that was isolated from kefir grain.

NCBI tax id

NCBI tax idMatching level
1423787strain
152332species

strain history

doi: 10.13145/bacdive6567.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lentilactobacillus
  • species: Lentilactobacillus parakefiri
  • full scientific name: Lentilactobacillus parakefiri (Takizawa et al. 1994) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus parakefir
    20215Lactobacillus parakefiri

@ref: 3924

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lentilactobacillus

species: Lentilactobacillus parakefiri

full scientific name: Lentilactobacillus parakefiri (Takizawa et al. 1994) Zheng et al. 2020

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 96.794

colony morphology

  • @ref: 54806
  • incubation period: 3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3924R - CW MEDIUM (DSMZ Medium 775)yeshttps://mediadive.dsmz.de/medium/775Name: R - CW MEDIUM (DSMZ Medium 775) Composition: Yeast extract 5.0 g/l Na-acetate 5.0 g/l KH2PO4 5.0 g/l Trypticase 5.0 g/l Tryptone 5.0 g/l (NH4)2 citrate 2.0 g/l MgSO4 x 7 H2O 0.5 g/l MnSO4 x H2O 0.2 g/l Tween 80 Cheese whey
41060MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)

culture temp

@refgrowthtypetemperaturerange
3924positivegrowth30mesophilic
41060positivegrowth30mesophilic
54806positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3924anaerobe
54806anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.761

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 93.372

murein

  • @ref: 3924
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3924kefir grainDenmarkDNKEurope
54806Kefir grains from E.C.Hansen's lab.,Copenhagen,DKDenmarkDNKEurope
67770Kefir grains

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production

taxonmaps

  • @ref: 69479
  • File name: preview.99_3539.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_367;97_831;98_967;99_3539&stattab=map
  • Last taxonomy: Lentilactobacillus
  • 16S sequence: LC096211
  • Sequence Identity:
  • Total samples: 4525
  • soil counts: 442
  • aquatic counts: 432
  • animal counts: 3255
  • plant counts: 396

Safety information

risk assessment

  • @ref: 3924
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus parakefiri gene for 16S rRNA, partial sequence, strain: JCM 8573AB289232610ena152332
20218Lactobacillus parakefiri gene for 16S ribosomal RNA, partial sequence, strain: JCM 8573AB3708791497ena152332
20218Lactobacillus parakefiri gene for 16S rRNA, partial sequence, strain: YIT 10382AB4293731533ena152332
20218Lactobacillus parakefiri 16S ribosomal RNA gene, partial sequenceAY0267501396ena152332
20218Lactobacillus parakefiri gene for 16S rRNA, partial sequence, strain: NBRC 15890AB6809911500ena152332
67770Lactobacillus parakefiri gene for 16S ribosomal RNA, partial sequence, strain: JCM 8573LC0962111514ena152332

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus parakefiri DSM 105511423787.3wgspatric1423787
66792Lactobacillus parakefiri strain DSM 10551152332.7wgspatric152332
66792Lactobacillus parakefiri strain JCM 8573152332.3wgspatric152332
66792Lentilactobacillus parakefiri DSM 105512660238146draftimg1423787
66792Lactobacillus parakefiri JCM 85732916479636draftimg152332
66792Lentilactobacillus parakefiri DSM 105512901596593draftimg1423787
67770Lentilactobacillus parakefiri DSM 10551 DSM 10551GCA_001434215scaffoldncbi1423787
67770Lentilactobacillus parakefiri JCM 8573GCA_002157585scaffoldncbi152332
67770Lentilactobacillus parakefiri DSM 10551GCA_004354625scaffoldncbi152332
66792Enterococcus faecium 248EA1GCA_003320275scaffoldpatric1352
66792Stenotrophomonas maltophilia AS012683GCA_010605085scaffoldpatric40324
66792Klebsiella pneumoniae TUM12129GCA_019703025completepatric573

GC content

@refGC-contentmethod
392442thermal denaturation, midpoint method (Tm)
6777042.6genome sequence analysis

External links

@ref: 3924

culture collection no.: CCUG 39468, GCL 1731, ATCC 51648, DSM 10551, IFO 15890, JCM 8573, NBRC 15890, CGMCC 1.3400, CIP 104242, KCTC 5044, KCTC 5087, LMG 15133, NCIMB 13300

straininfo link

@refpassport
20218http://www.straininfo.net/strains/162077
20218http://www.straininfo.net/strains/54578
20218http://www.straininfo.net/strains/54579
20218http://www.straininfo.net/strains/335570

literature

  • Pubmed-ID: 28748134
  • title: Genomic characterization reconfirms the taxonomic status of Lactobacillus parakefiri.
  • authors: Tanizawa Y, Kobayashi H, Kaminuma E, Sakamoto M, Ohkuma M, Nakamura Y, Arita M, Tohno M
  • journal: Biosci Microbiota Food Health
  • DOI: 10.12938/bmfh.16-026
  • year: 2017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3924Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10551)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10551
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41060Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16129
54806Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39468)https://www.ccug.se/strain?id=39468
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)